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A kind of method of extracting rutin in Sophora japonica

A technology of sophora japonica and rutin is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., to achieve the effects of shortening extraction time, improving purity, and improving extraction efficiency.

Active Publication Date: 2016-07-27
内蒙古昶辉生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are reports on the use of surfactants to assist the extraction of rutin, but more than one surfactant is used

Method used

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  • A kind of method of extracting rutin in Sophora japonica
  • A kind of method of extracting rutin in Sophora japonica
  • A kind of method of extracting rutin in Sophora japonica

Examples

Experimental program
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Effect test

Embodiment 1

[0018] 50g of dried Sophora japonica was treated by dry steaming at 70°C for 30min, then rinsed with hot water at 70°C to remove the gelatin, then dried in the air and crushed into 20 meshes. Add 3% borax and 1% Na to Sophora japonica granules 2 SO 3 and 20 times of 60-70% ethanol, use sodium bicarbonate to adjust the pH to 9-10, then add 0.35% Tween20 and 0.15% OP-15, and conduct ultrasonic extraction at 30kHz and 40°C for 20min in the dark. After filtering, the filtrate was kept in the dark at 40°C for later use; the filter residue was extracted once again under the same conditions as above, and the obtained filtrate was combined with the previous filtrate; 5% activated carbon was added to the combined filtrate, and stirred for 10 minutes at 40°C in the dark, and the The filtrate after filtering to remove charcoal was acidified to pH = 3-4, allowed to stand for 20 minutes, and filtered with suction to obtain crude rutin. Place the crude rutin in an oven, dry it at 40°C unt...

Embodiment 2

[0020] 80g of dried Sophora japonica was treated by dry steaming method at 70°C for 30min, then rinsed with hot water at 70°C to remove the gelatin, then dried in the air and crushed into 40 meshes. Add Sophora japonica granules with 4% borax and 1% Na 2 SO 3 and 25 times of 60-70% ethanol, using sodium bicarbonate to adjust the pH to 9-10, then adding 0.40% Tween20 and 0.10% OP-15, and ultrasonically extracting at 30kHz and 40°C for 20min in the dark, After filtering, the filtrate was kept in the dark at 40°C for later use; the filter residue was extracted once again under the same conditions as above, and the obtained filtrate was combined with the previous filtrate; 5% activated carbon was added to the combined filtrate, and stirred for 10 minutes at 40°C in the dark, and the The filtrate after filtering to remove charcoal was acidified to pH = 3-4, allowed to stand for 20 minutes, and filtered with suction to obtain crude rutin. Place the crude rutin in an oven, dry it a...

Embodiment 3

[0022] 120g of dried Sophora japonica was treated by dry steaming method at 70°C for 30min, then rinsed with hot water at 70°C to remove the gelatin, then dried in the air and crushed into 60 meshes. Add 3% borax and 1% Na to Sophora japonica granules 2 SO 3 and 30 times of 60-70% ethanol, using sodium bicarbonate to adjust the pH to 9-10, then adding 0.45% Tween20 and 0.05% OP-15, and ultrasonically extracting at 30kHz and 40°C for 20min in the dark, After filtering, the filtrate was kept in the dark at 40°C for later use; the filter residue was extracted once again under the same conditions as above, and the obtained filtrate was combined with the previous filtrate; 5% activated carbon was added to the combined filtrate, and stirred for 10 minutes at 40°C in the dark, and the The filtrate after filtering to remove charcoal was acidified to pH = 3-4, allowed to stand for 20 minutes, and filtered with suction to obtain crude rutin. Place the crude rutin in an oven, dry it at...

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Abstract

The invention discloses a new method for extracting rutin from Sophora japonica, comprising the following steps: drying and pulverizing the dried Sophora japonica at 70°C by dry steaming; adding surfactant Tween with a reasonable ratio 20 and OP-15, etc., use 60% to 70% ethanol to carry out ultrasonic-assisted extraction under certain conditions, let the extract stand for crystallization, and suction filter to obtain crude rutin, refine the crude rutin with n-butanol, let stand Crystallize and dry at 130°C to obtain a rutin product with a purity of over 98%, with a yield of over 22%. Based on the above, the method of the present invention is easy to operate, has high extraction efficiency, and is suitable for actual production.

Description

technical field [0001] The application relates to a method for extracting rutin from Sophora japonica. Background technique [0002] Sophora japonica is the dried flowers and flower buds of Sophora japonica, a leguminous plant. It has been used as medicine for more than 2,000 years. It is listed as top grade in "Shen Nong's Materia Medica". The rutin contained in Sophora japonica is about 8% to 28% (varies according to the place of origin and harvesting time of Sophora japonica). Rutin, also known as rutin and purple quercitrin, belongs to flavonoid glycosides. It has the effects of cooling blood and stopping bleeding, clearing liver and purging fire, reducing capillary permeability and fragility, promoting cell proliferation and preventing blood cell aggregation. Anti-inflammatory, anti-allergic, diuretic, antitussive, hypolipidemic and other effects. Rutin has been used clinically as an adjuvant treatment for hypertension, to prevent other bleeding disorders caused by ru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 梁玉强马亚琼白易张成亮赵永强
Owner 内蒙古昶辉生物科技股份有限公司
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