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Dendrobium officinale culture medium and preparation method thereof

A technology of dendrobium candidum and culture medium, which is applied in botany equipment and methods, biochemical equipment and methods, tissue culture, etc., can solve the problems of unfavorable market sales of dendrobium candidum, reduced seedling quality, unsatisfactory effect, etc., and achieve effective Conducive to rooting and strong seedlings, quality improvement, easy to obtain effects

Active Publication Date: 2016-05-18
ZHANGZHOU XINZHENYU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many kinds of media for the tissue culture of Dendrobium candidum in the market, and the content of the ingredients is not the same, but the effect of these media on the rooting stage of the strong seedlings of Dendrobium candidum is not ideal, the stems are not strong enough, the root system is not enough, etc., making Seedling quality is greatly reduced, which is not conducive to the market sales of Dendrobium officinale
And its preparation required medicine is numerous, and preparation is comparatively troublesome

Method used

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  • Dendrobium officinale culture medium and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) According to the configuration of 10 liters of the dendrobium candidum culture medium, weigh Huabao No.1 35000mg, citric acid 1000mg, inositol 200mg, vitamin B1200mg, potassium dihydrogen phosphate 1000mg, peptone 20000mg, potato 700000mg, banana 700000mg, sugar 300000mg , Agar 65000mg, activated carbon 10000mg;

[0034] 2) Add Huabao No. 1, citric acid, inositol, vitamin B1, potassium dihydrogen phosphate and peptone to 2 liters of deionized water at room temperature, and stir until dissolved;

[0035] 3) Potatoes are cooked in 2 liters of deionized water and then crushed, and added to the solution obtained in step 2) together with the solvent;

[0036] 4) After the banana is crushed, add it to the solution obtained in step 3);

[0037] 5) Add white sugar and agar to 3 liters of deionized water at room temperature, stir the white sugar to dissolve, stir the agar to mix, and add to the solution obtained in step 4);

[0038] 6) Add activated carbon to the solution obtained in ...

Embodiment 2

[0044] 1) According to the configuration of 10 liters of the dendrobium candidum culture medium, weigh out Huabao No.1 30000mg, citric acid 500mg, inositol 150mg, vitamin B1150mg, potassium dihydrogen phosphate 500mg, peptone 15000mg, potato 500000mg, banana 500000mg, white sugar 300000mg , Agar 60000mg, activated carbon 15000mg;

[0045] 2) Add Huabao No. 1, citric acid, inositol, vitamin B1, potassium dihydrogen phosphate and peptone to 2 liters of deionized water at room temperature, and stir until dissolved;

[0046] 3) Potatoes are cooked in 2 liters of deionized water and then crushed, and added to the solution obtained in step 2) together with the solvent;

[0047] 4) After the banana is crushed, add it to the solution obtained in step 3);

[0048] 5) Add white sugar and agar to 3 liters of deionized water at room temperature, stir the white sugar to dissolve, stir the agar to mix, and add to the solution obtained in step 4);

[0049] 6) Add activated carbon to the solution obta...

Embodiment 3

[0053] 1) According to the configuration of 10 liters of the dendrobium candidum culture medium, weigh Huabao No. 1 40000mg, citric acid 2000mg, inositol 250mg, vitamin B1250mg, potassium dihydrogen phosphate 2000mg, peptone 25000mg, potato 1000000mg, banana 1000000mg, white sugar 200000mg , Agar 70,000mg, activated carbon 20,000mg;

[0054] 2) Add Huabao No. 1, citric acid, inositol, vitamin B1, potassium dihydrogen phosphate and peptone to 2 liters of deionized water at room temperature, and stir until dissolved;

[0055] 3) Potatoes are cooked in 2 liters of deionized water and then crushed, and added to the solution obtained in step 2) together with the solvent;

[0056] 4) After the banana is crushed, add it to the solution obtained in step 3);

[0057] 5) Add white sugar and agar to 3 liters of deionized water at room temperature, stir the white sugar to dissolve, stir the agar to mix, and add to the solution obtained in step 4);

[0058] 6) Add activated carbon to the solution o...

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Abstract

The invention discloses a dendrobium candidum culture medium and a preparation method thereof. The culture medium comprises Huabao No.1 with the concentration in a culture medium solution is 3-4g / L; organic elements such as citric acid, inositol and vitamin B1, wherein the concentration of organic element in the culture medium solution is 15-200mg / L; a compound such as potassium dihydrogen phosphate, with the concentration in the culture medium solution being 50-300mg / L; and additives such as peptone, potatoes and bananas, wherein the concentration of each material in the culture medium solution is 0.5-100g / L. The culture medium disclosed by the invention is simple in composition, contains multiple nutrients, and simple and convenient to prepare, can well promote strong seedling and rooting growth of dendrobium candidum so that the stems grow thick and strong, the root systems are well developed, and further the quality of seedlings out of bottles are effectively improved.

Description

Technical field [0001] The invention relates to a Dendrobium officinale culture medium and a preparation method thereof. Background technique [0002] Dendrobium is a perennial herbaceous plant of the genus Dendrobium of the orchid family, and is the largest genus in the orchid family. Dendrobium is naturally a very vigorous plant. It mostly grows on inaccessible cliffs and grows on trees or in the crevices of rocks. It enjoys a warm, humid and half-yin and half-yang climate. There are more than 1,600 species of Dendrobium in the world, and there are about 76 species in my country, most of which are distributed in Southwest, South China, Taiwan and other places. Dendrobium officinale is one of many kinds of dendrobium, also known as yellow grass and black knotweed. It is the best product in dendrobium. It is named for its iron-green surface and has unique medicinal value. It is commonly called "life-saving fairy grass". Because it contains a large number of Dendrobium polysacch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
Inventor 庄国钟林伯煌
Owner ZHANGZHOU XINZHENYU BIOTECH
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