The invention discloses a substrate clamp for vertical double-sided film coating. The substrate clamp for vertical double-sided film coating comprises a lower tray, an upper tray, a substrate, clamping devices and a main body bracket, wherein the clamping device is locked on the main body bracket, the substrate is clamped between the lower tray and the upper tray, and the substrate is clamped between the upper tray and the lower tray; the lower tray and the upper tray are clamped and attached through the clamping devices, hollowed-out sheet positions are arranged on the lower tray and the upper tray, the edges of the hollowed-out sheet positions extend outwards to be provided with edge stop blocks, the four corners of the hollowed-out sheet positions are provided with four-corner stop blocks, and trapezoidal bosses are arranged on the edges of a frame on the periphery of the upper tray; and trapezoidal grooves matched with the trapezoidal bosses are formed in the lower tray, and the substrate is limited in a narrow space in the middle by the edge stop blocks and the four-corner stop blocks. In this way, the phenomena of wafer falling and wafer inclining are not liable to happen; double-sided film coating with the maximum area is facilitated; a film coating shielding area is reduced, and a substrate film coating invalid area is reduced; and the clamping design of springs and baffles facilitates the realization of automatic mass production operation.