The invention discloses a blood vessel dynamic mechanical response testing system, which comprises a base, a pulsating flow field driving device, a monitoring device and a transmission device, whereinthe output end of the pulsating flow field driving device is connected with the first input end of the transmission device, the monitoring device is arranged on the base, a tested sample is arrangedon the monitoring device, the two ends of the tested sample are connected with the first output end and the second input end of the conveying device respectively, the second output end of the conveying device is connected with the input end of the pulsating flow field driving device, the pulsating flow field driving device is used for pumping a periodic pulsating fluid into the tested sample through the transmission device, and the monitoring device is used for collecting the three-dimensional dynamic mechanical response data of the tested sample after the periodic pulsating fluid is pumped. According to the invention, with the system, the blank that dynamic mechanical testing cannot be conducted on blood vessels or vascular grafts in a three-dimensional state or a native state on the market at present is filled up, the structure is simple and compact, the space design is reasonable, operation is very easy and convenient, and great convenience is brought to use of a user.