32results about How to "Preserve the characteristic structure" patented technology

The invention aims to provide a piroxicam hapten and a synthesis method and application thereof, belonging to the field of drug safety detection. The preparation method comprises the following steps:1) mixing saccharin and ethyl bromoacetate for reacting to prepare an intermediate product 1; 2) mixing and extracting the intermediate product 1 with DMSO and MeONa to obtain an intermediate product2; 3) mixing the intermediate product 2 and CH3I for reacting to obtain an intermediate product 3; 4) mixing the intermediate product 3 and 6-aminonicotinic acid methyl ester for reacting to obtain anintermediate product 4; 5) after adding of the intermediate product 4 into a reagent for miscibility reaction, purifying to obtain the final product, namely the piroxicam hapten. Immunological detection method has the advantages of high sensitivity, strong specificity, simple sample pretreatment, short detection time, strong stability, no need of special equipment and reagents, intuitive judgmentof results and the like, thus being particularly suitable for rapid detection of illegal addition of piroxicam chemical drugs in vast basic inspection personnel and large quantities of anti-rheumatism Chinese patent medicines.

The invention discloses an artificial zopiclone hapten, an artificial zopiclone antigen, a preparation method of the artificial zopiclone antigen and application of the artificial zopiclone antigen inpreparation of a zopiclone antibody. The molecular structural formula of the artificial zopiclone hapten is represented by formula (I), and the molecular structural formula of the artificial zopiclone antigen is represented by formula (II). The artificial zopiclone hapten maintains the characteristic structure of zopiclone to the greatest extent, contains active groups which can be coupled with carrier protein, and can be used as an antigenic determinant; the zopiclone antibody with high affinity, sensitivity and specificity can be prepared from the further obtained zopiclone artificial antigen in an immune manner, the valence of immune serum obtained from an immune white rabbit of New Zealand is 1:70000, and the zopiclone antibody can be used for rapid and accurate immunodetection and immunoassay of the zopiclone.

The invention discloses a propiconazole hapten as well as a preparation method and application thereof. According to the propiconazole hapten, a de novo synthesis method is adopted, a connecting arm containing four C atoms is connected to a benzene ring of propiconazole, and a carboxyl group capable of being coupled with carrier protein is introduced to the tail end of the connecting arm. The monoclonal antibody prepared by using the hapten is high in sensitivity and strong in specificity. The sensitivity (IC50) measured by an indirect competitive enzyme-linked immunoassay (ic-ELISA) method is 2.33 [mu] g / L, and the detection range (IC10-IC90) is 0.26-21.28 [mu] g / L. The monoclonal antibody and the propiconazole analogue have no cross reaction (CR is less than 0.1%). In addition, the monoclonal antibody is used for preparing the propiconazole colloidal gold rapid detection test strip which is high in detection sensitivity, strong in specificity, simple, convenient, rapid and low in cost. The test strip can be used for detecting propiconazole residues in agricultural products, and the detection limit of the test strip reaches 5 [mu] g / L.

The invention discloses an amantadine artificial hapten, artificial antigen, a preparation method and application thereof. The molecular structural formula of the amantadine artificial hapten is shown in formula (I), and the molecular structural formula of the amantadine artificial antigen is shown in formula (II). The application is the application of the amantadine artificial antigen in the preparation of anti-amantadine antibody. The amantadine artificial hapten of the present invention retains the characteristic structure of amantadine to the greatest extent, and has an active group that can be coupled with a carrier protein, which can be used as an antigenic determinant; the obtained amantadine artificial antigen is further prepared The anti-amantadine antibody with high affinity, high sensitivity and strong specificity can be obtained by immunization, and the titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:70000, which can be used for rapid and accurate immunological detection and detection of amantadine immunoassay.

The invention discloses preparation methods and application of semicarbazide SEM derived hapten and artificial antigen. An active arm introduced by the hapten integrally keeps a characteristic structure of an SEM derivative and has no influences on electronic cloud density; the hapten also has an active group capable of being coupled with a carrier protein; reaction conditions are moderate and operation steps are simple; the prepared artificial antigen can be specifically combined with an antibody, and has the characteristics of high valence, high sensitivity, high accuracy and the like; and residues of semicarbazide SEM in various products can be rapidly detected.

The invention discloses an artificial zopiclone hapten, an artificial zopiclone antigen, a preparation method of the artificial zopiclone antigen and application of the artificial zopiclone antigen inpreparation of a zopiclone antibody. The molecular structural formula of the artificial zopiclone hapten is represented by formula (I), and the molecular structural formula of the artificial zopiclone antigen is represented by formula (II). The artificial zopiclone hapten maintains the characteristic structure of zopiclone to the greatest extent, contains active groups which can be coupled with carrier protein, and can be used as an antigenic determinant; the zopiclone antibody with high affinity, sensitivity and specificity can be prepared from the further obtained zopiclone artificial antigen in an immune manner, the valence of immune serum obtained from an immune white rabbit of New Zealand is 1:70000, and the zopiclone antibody can be used for rapid and accurate immunodetection and immunoassay of the zopiclone.

The invention discloses a clarithromycin hapten, an artificial antigen and an antibody, and a preparation method and application thereof. The invention aims to provide the clarithromycin hapten which can maintain the feature structure of clarithromycin to the greatest degree and has an active group capable of coupling with carrier protein; the artificial antigen and antibody are prepared from the clarithromycin hapten to be used for detecting macrolide compounds; according to the technical scheme, the structure of the clarithromycin hapten is as shown in the following formula; the clarithromycin artificial antigen is prepared by coupling the clarithromycin hapten with the carrier protein; the invention belongs to the field of a biotechnology; and (the formula is as shown in the description).

The invention discloses fluazinam hapten, artificial antigen and antibody, and preparation methods and applications thereof. The fluazinam hapten provided by the invention not only retains the characteristic structure of fluazinam, but also makes the immune The originality is obviously enhanced, and it has a carboxyl group that can be coupled with the carrier protein; the fluazinam immunization antigen obtained by coupling the fluazinam hapten with the carrier protein is used to immunize animals, which is more conducive to stimulating the animal's immune response to produce specificity A stronger and more sensitive antibody, the sensitivity of the antibody to fluazinam can reach 0.1 μg / L, and the cross-reaction rate with other pesticides is low, which provides a basis for the subsequent establishment of various immunoassay methods for fluazinam.

The invention discloses a pregabalin artificial hapten, an artificial antigen as well as a preparation method and application of the pregabalin artificial hapten and the artificial antigen. The molecular structural formula of the pregabalin artificial hapten is as shown in formula (I), and the molecular structural formula of the pregabalin artificial antigen is as shown in formula (II). The application refers to the application of the pregabalin artificial antigen in preparation of an anti-pregabalin antibody. The pregabalin artificial hapten maximally retains the characteristic structure of pregabalin, has an active group capable of being coupled with carrier protein, and can be used as an antigenic determinant; the further prepared pregabalin artificial antigen can be immunized to obtain an anti-pregabalin antibody with high affinity, high sensitivity and strong specificity, the titer of immune serum obtained by immunizing a New Zealand white rabbit reaches up to 1: 90000, and the pregabalin artificial antigen can be used for rapid and accurate immunodetection and immunoassay of pregabalin.

The invention discloses a prochloraz hapten, an artificial antigen and an antibody as well as a preparation method and application thereof. The prochloraz hapten provided by the invention not only retains the characteristic structure of the prochloraz hapten to the greatest extent, and makes the immunization of the prochloraz hapten The originality is obviously enhanced, and it has a carboxyl group that can be coupled with the carrier protein; the prochloraz immunization antigen obtained by coupling the prochloraz hapten with the carrier protein is used to immunize animals, which is more conducive to stimulating the animal's immune response to produce specificity Stronger and more sensitive antibody, the sensitivity of prochloraz antibody can reach 0.1μg / L, and the cross-reaction rate with other pesticides is low, which provides a basis for the subsequent establishment of various immunoassay methods for prochloraz.

The invention discloses a difenoconazole hapten, an artificial antigen and an antibody as well as a preparation method and application thereof. The difenoconazole hapten provided by the invention not only retains the characteristic structure of The immunogenicity of the difenoconazole hapten is obviously enhanced, and it has a carboxyl group that can be coupled with the carrier protein; the difenoconazole artificial antigen obtained by coupling the difenoconazole hapten with the carrier protein is used to deimmunize Animals are more conducive to stimulating the immune response of animals to produce antibodies with higher specificity and higher sensitivity. The sensitivity of difenoconazole antibodies can reach 0.05 μg / L after detection, and the cross-reaction rate with other triazole pesticides is low. It provides a basis for the subsequent establishment of various immunoassay methods for difenoconazole.

The invention discloses an ethyl sulfate artificial antigen, a preparation method and an application thereof. The invention uses 2-aminoethanol hydrogen sulfate as a raw material to prepare an ethyl sulfate artificial antigen, and introduces 2-(4-(methyl bromide) into the amino group. base) phenyl) acetic acid linking arm to prepare an ethyl sulfate hapten, which reacts with N-hydroxysuccinimide and N,N-cyclohexylcarbodiimide to obtain an active ester, which is combined with The protein coupling reaction was carried out to obtain ethyl sulfate artificial antigen. The present invention only needs one step to obtain the hapten, the process is simple, the source of raw materials is not limited, and it also retains the characteristic structure of ETS, which has good specificity; the linker introduced by the present invention can maximize the potential of the antigen Immunogenicity, enhance the immune effect, stimulate animals to produce antibodies, and can effectively make up for the shortcomings of ETS, which is difficult to stimulate the body to produce antibodies because of its too simple structure.

The invention provides an imidacloprid hapten, a complete antigen and synthesis and application of the imidacloprid hapten and the complete antigen. The invention relates to the imidacloprid hapten and the synthesis method thereof; the synthesis method of the imidacloprid hapten is simple and efficient; and compared with the prior art, the synthesis method can save the synthesis cost of the imidacloprid hapten and improve the synthesis efficiency of the imidacloprid hapten. The invention also relates to the imidacloprid complete antigen and two synthesis methods thereof. The imidacloprid complete antigen basically retains the characteristic structure of imidacloprid.

The present invention discloses sorbic acid hapten, artificial antigen, antibody and its synthesis method and application. In the present invention, two kinds of sorbic acid haptens with different structures are provided, wherein the sorbic acid hapten with the structure shown in formula () is used as The hapten for sorbic acid immunization, the hapten for sorbic acid immunization is highly similar to the target analyte sorbic acid, retains the characteristic structure of sorbic acid to the greatest extent, and can better expose the epitope of the hapten, making sorbic acid The immunogenicity of the hapten is obviously enhanced, and it has an aldehyde group that can be coupled with the carrier protein; the sorbic acid immunization antigen obtained after the hapten is coupled with the carrier protein is used to immunize animals, which is more conducive to stimulating animals. The immune response produces antibodies with stronger specificity and higher sensitivity, and the IC of sorbic acid antibody is detected by indirect competitive ELISA 50 It is 11mg / L, which provides the basis for the subsequent establishment of various immunoassay methods for sorbic acid.

The invention discloses a baclofen artificial hapten, an artificial antigen and a preparation method and application thereof. The molecular structural formula of the baclofen artificial hapten is as formula (I), and the molecular structural formula of the baclofen artificial antigen is as formula (II). The baclofen artificial hapten of the present invention retains the characteristic structure of baclofen to the greatest extent, and has an active group that can be coupled with a carrier protein, and can be used as an antigenic determinant; the baclofen artificial antigen obtained by further preparation Anti-baclofen antibodies with high affinity, high sensitivity and specificity can be obtained by immunization. The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:80,000, which can be used for rapid and accurate immunological detection and detection of baclofen. Immunoassay.

The invention discloses a clarithromycin derivative, and a preparation method and application thereof. The invention aims to provide the clarithromycin clarithromycin derivative which can maintain the feature structure of clarithromycin to the greatest degree and has an active group capable of coupling with carrier protein; an artificial antigen and an antibody are prepared from the clarithromycin derivative to be used for detecting macrolide compounds; according to the technical scheme, the structure of the clarithromycin derivative is as shown in the following formula; the clarithromycin artificial antigen is prepared by coupling the clarithromycin derivative with the carrier protein; the invention belongs to the field of a biotechnology; and (the formula is as shown in the description).