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Fluoketamine artificial hapten, artificial antigen and preparation method and application thereof

A technology of artificial hapten and artificial antigen, which is applied in the field of artificial antigen and its preparation, fluoroamine artificial hapten, can solve the problems of expensive equipment and time-consuming detection, and achieve high affinity, strong specificity, accurate immune detection and immune The effect of the analysis

Pending Publication Date: 2022-02-08
HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to overcome the shortcomings of expensive instruments, time-consuming detection and the need for professional technicians to operate in the detection methods of fluoroamine in the prior art, and provides a fluoroamine artificial hapten, artificial antigen and preparation thereof Methods and applications to overcome the aforementioned deficiencies

Method used

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  • Fluoketamine artificial hapten, artificial antigen and preparation method and application thereof
  • Fluoketamine artificial hapten, artificial antigen and preparation method and application thereof
  • Fluoketamine artificial hapten, artificial antigen and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The implementation of a preparation method of fluoroamine artificial antigen (reaction process as follows figure 1 ), including the following steps:

[0067] Preparation of artificial haptens:

[0068] ① Weigh 200mg (0.777mmol) of fluoroquinone hydrochloride and dissolve it in 20ml of purified water, add ammonia water drop by drop to adjust the pH of the solution to 9. At this time, the solution is a white turbid liquid, extract it with 20ml*3 dichloromethane, and collect the organic phase, dried with anhydrous magnesium sulfate, filtered, and evaporated to dryness under reduced pressure to obtain 165mg (0.747mmol) of colorless oil A;

[0069] The colorless oil A was detected by TLC, the chromatographic solution was ethyl acetate:petroleum ether=1:1 (v / v), the product R f =0.3.

[0070] ② Dissolve 165mg (0.747mmol) of the colorless oily substance A in the previous step in 5ml of pyridine, add a stir bar, then add 170mg (1.491mmol) glutaric anhydride, stir and reflux ...

Embodiment 2

[0084] The implementation of a preparation method of fluoroamine artificial antigen (reaction process as follows Figure 5 ), including the following steps:

[0085] (1) Preparation of artificial hapten of fluoroamine:

[0086] ①Same as Example 1.

[0087] ②Dissolve 183mg (0.826mmol) of the colorless oily substance A in the previous step in 5ml of pyridine, add a stirrer, then add 82.6mg (0.826mmol) of succinic anhydride, stir and reflux in an oil bath at 90°C for 24 hours;

[0088] After the reaction was completed, the solvent was evaporated to dryness under reduced pressure, and azeotroped with 10 ml of absolute ethanol. Add 30ml of dichloromethane to dissolve the residue, then wash the organic phase with 20ml*3 purified water, collect the organic phase, dry with anhydrous magnesium sulfate, filter, and evaporate to dryness under reduced pressure to obtain a brown-black oily substance, which is thin-layer chromatographed (chromatographic liquid is Dichloromethane: 95% eth...

Embodiment 3

[0093] The implementation of a preparation method of fluoroamine artificial antigen (reaction process as follows Figure 6 ), including the following steps:

[0094] (1) Preparation of artificial hapten of fluoroamine:

[0095] ①Same as Example 1.

[0096] ②Dissolve 173mg (0.782mmol) of the colorless oily substance A in the previous step in 5ml of pyridine, add a stirrer, then add 300mg (2.346mmol) of adipic anhydride, stir and reflux in an oil bath at 110°C for 15 hours;

[0097] After the reaction was completed, the solvent was evaporated to dryness under reduced pressure, and azeotroped with 10 ml of absolute ethanol. Add 30ml of dichloromethane to dissolve the residue, then wash the organic phase with 20ml*3 purified water, collect the organic phase, dry with anhydrous magnesium sulfate, filter, and evaporate to dryness under reduced pressure to obtain a brown-black oily substance, which is thin-layer chromatographed (chromatographic liquid is Dichloromethane: 95% ethan...

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Abstract

The invention belongs to the technical field of biochemical engineering, and particularly relates to a fluoketamine artificial hapten, an artificial antigen and a preparation method and application thereof. The fluoketamine artificial hapten maximally retains the characteristic structure of the fluoketamine, and has an active group capable of being coupled with carrier protein after chemical modification so that the fluoketamine artificial antigen can be obtained; the further prepared fluoketamine artificial antigen can be used for immunizing to obtain an anti-fluoketamine antibody with high affinity, high sensitivity and strong specificity; the antigen and the antibody can be used for an immunochromatography technology, have the advantages of high sensitivity, strong specificity, easiness in operation, economy, rapidness and the like, and can be used for preliminary screening of the abuse of the fluoketamine.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a fluoroamine artificial hapten, an artificial antigen and a preparation method and application thereof. Background technique [0002] The chemical name of F-Ketamine is 2-o-fluorophenyl-2-methylaminocyclohexanone, which belongs to the phencyclidine class of substances like ketamine, and its molecular structure is also highly similar to ketamine. Preliminary pharmacological experiments show that flutamine has similar anesthetic activity to ketamine and is a new anesthetic active substance. Its clinical features include stiffness, light sedation, amnesia, significant analgesia, entering dreams, and hallucinations. [0003] In recent years, domestic sources of ketamine have become less and less, and fluoramine, as its substitute, has been abused by drug addicts. Suspected drugs containing this substance have been found in various parts of the country. In 2020, re...

Claims

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Application Information

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IPC IPC(8): C07C233/32C07C231/02C07C221/00C07C225/20C07K14/765C07K1/107C07K16/44C07K16/06G01N33/94
CPCC07C233/32C07C231/02C07C221/00C07K14/765C07K16/44C07K16/06G01N33/94C07C2601/14C07C225/20
Inventor 王镇邵越水
Owner HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD
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