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Trazodone artificial hapten, artificial antigen and preparation method and application thereof

An artificial hapten, artificial antigen technology, applied in chemical instruments and methods, animal/human proteins, peptides, etc., can solve the problems of expensive trazodone instruments, time-consuming detection, inability to meet the requirements of rapidity and accuracy, and achieve accurate Effects of immunoassays and immunoassays, high affinity, and high sensitivity

Pending Publication Date: 2022-07-22
HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to overcome the disadvantages of expensive instruments, time-consuming detection and operation by professional technicians in the detection of trazodone in the prior art, which cannot meet the fast and accurate requirements of modern detection, and provides a kind of trazodone Artificial hapten, artificial antigen and its preparation method and application to overcome the above-mentioned defects

Method used

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  • Trazodone artificial hapten, artificial antigen and preparation method and application thereof
  • Trazodone artificial hapten, artificial antigen and preparation method and application thereof
  • Trazodone artificial hapten, artificial antigen and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] This implements a preparation method of trazodone artificial antigen (reaction process such as figure 1 ), including the following steps:

[0079] (1) Preparation of artificial hapten I:

[0080] ①Weigh 1g (7.41mmol) of pyridinetriazolone into a 50ml three-necked round bottom flask, add 10ml of N,N-dimethylformamide, pass nitrogen into the left port, connect a condenser tube and a drying tube to the middle port, and plug the right port with a rubber At this time, the solution was a yellow suspension, which was slowly heated to 80°C in an oil bath, and the solution was a yellow transparent solution at this time. Then, 296 mg (7.41 mmol) of 60% sodium hydride was added, and a large amount of precipitation was produced at this time. The reactant was continuously stirred at 80 ° C for 1 hour, and then 1-chloro-3-iodopropane was slowly added from the right port with a syringe. 1.2ml (11.12mmol), then the reaction solution was heated to 100°C under reflux and stirred for 6 ...

Embodiment 2

[0100] This implements a preparation method of trazodone artificial antigen (reaction process such as figure 1 ), including the following steps:

[0101] (1) Preparation of artificial hapten I:

[0102] ①Weigh 1g (7.41mmol) of pyridinetriazolone in a 50ml three-necked round bottom flask, add 10ml N,N-dimethylformamide, pass nitrogen into the left port, connect a condenser tube and a drying tube to the middle port, and plug the right port with a rubber At this time, the solution was a yellow suspension, which was slowly heated to 80°C in an oil bath, and the solution was a yellow transparent solution at this time. Then, 296 mg (7.41 mmol) of 60% sodium hydride was added, and a large amount of precipitation was produced at this time. The reactant was continued to be stirred at 70 °C for 2 hours under reflux, and then 1-chloro-3-iodopropane was slowly added from the right port with a syringe. 0.8 ml (7.41 mmol), then the reaction solution was heated to 95°C under reflux and sti...

Embodiment 3

[0118] This implements a preparation method of trazodone artificial antigen (reaction process such as figure 1 ), including the following steps:

[0119] (1) Preparation of artificial hapten I:

[0120] ①Weigh 1g (7.41mmol) of pyridinetriazolone into a 50ml three-necked round bottom flask, add 10ml of N,N-dimethylformamide, pass nitrogen into the left port, connect a condenser tube and a drying tube to the middle port, and plug the right port with a rubber At this time, the solution was a yellow suspension, which was slowly heated to 80°C in an oil bath, and the solution was a yellow transparent solution at this time. Then, 296 mg (7.41 mmol) of 60% sodium hydride was added, and a large amount of precipitation was produced at this time. The reactant was continuously stirred at 85 °C for 0.5 hours under reflux, and then 1-chloro-3-iodopropane was slowly added from the right port with a syringe. (1~2 times) 1.6 ml (14.82 mmol), then the reaction solution was heated to 105°C un...

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Abstract

The invention discloses a trazodone artificial hapten, an artificial antigen as well as a preparation method and application of the artificial hapten and the artificial antigen. The molecular structural formula of the trazodone artificial hapten is as shown in formula (I), and the molecular structural formula of the trazodone artificial antigen is as shown in formula (II). The application is the application of the trazodone artificial antigen in preparation of an anti-trazodone antibody. The trazodone artificial hapten maximally retains the characteristic structure of trazodone, has an active group capable of being coupled with carrier protein, and can be used as an antigenic determinant; the further prepared trazodone artificial antigen can be immunized to obtain an anti-trazodone antibody with high affinity, high sensitivity and strong specificity, the titer of immune serum obtained by immunizing a New Zealand white rabbit is very high, and the trazodone artificial antigen can be used for rapid and accurate immunodetection and immunoassay of trazodone.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a trazodone artificial hapten, an artificial antigen and a preparation method and application thereof. Background technique [0002] Trazodone is an antidepressant. For the treatment of various types of depression and anxiety disorders with depressive symptoms and mood disorders after drug withdrawal. Common adverse reactions of taking trazodone are drowsiness, fatigue, dizziness, insomnia, nervousness and tremor; and blurred vision, dry mouth, and constipation. Rarely, orthostatic hypotension (reduced by concomitant medication with meals), tachycardia, nausea, vomiting, and abdominal discomfort. Very few patients present with musculoskeletal pain and dreaminess. [0003] Some adverse reactions have been reported in clinical studies that may be related to the use of trazodone hydrochloride: akathisia, allergic reactions, anemia, flatulence, abnormal ur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D471/04C07K14/765C07K16/06C07K16/44
CPCC07D471/04C07K14/765C07K16/44C07K16/06C07K2317/35C07K2317/92
Inventor 邵越水王镇阮天宇
Owner HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD
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