36results about How to "Increased enzyme content" patented technology

The invention provides a preparation method of fruit ferment. The preparation method comprises the following steps: carrying out anaerobic fermentation by inoculating anaerobic bacterium strains, filtering the obtained fermentation products, saving the filtrate (A), keeping on inoculating aerobic bacterium strains to peels and dregs of fruits to carry out aerobic fermentation for 5 to 15 days, then adding liquor, keeping on fermentation for 5 to 10 days, then filtering the fermentation products, condensing the filtrates, then merging the condensed filtrates, adding molasses, inoculating beneficial bacteria to the mixed filtrate, and sealing the mixed filtrate for 10 to 20 days to obtain the environment-friendly ferment. The preparation method can effectively avoid the mildew of fruit peels during the fermentation process, thus the content of nitrite is reduced, the obtained ferment prepared from fruit peels is healthy and unique, at the same time, the ferment content is increased, the function of the ferment is improved, moreover, the wastes (fruit peels) are converted into valuable resources, and the utilization of wastes is promoted.

The invention discloses a process for preparing a tea enzyme liquid from fresh tea leaves and belongs to the field of preparation of tea source drinks. The fresh tea leaves are taken, cleaned, rolled and mixed with a yeast impregnating solution, 9% (V / V) rice vinegar and brown sugar are added, the mixture is uniformly stirred, fermented for more than one year in a shady and dry place, centrifuged and filtered, and the tea enzyme liquid is obtained. The fresh tea leaves are leached in water at the normal temperature, saccharomycetes, the rice vinegar and the brown sugar are combined for fermentation, and the tea enzyme liquid is prepared, doesn't lose nutritional ingredients and is healthier, safer and more efficient.

The invention discloses natural fruit and vegetable enzyme compound beverage and a preparation method. The natural fruit and vegetable enzyme compound beverage comprises a raw material and an auxiliary material, wherein the raw material comprises the following components in percentages by weight: 1-2% of apples, 1-2% of pears, 1-2% of pineapples, 1-2% of lemons, 1-2% of figs, 1-2% of hami melons, 1-2% of bananas, 1-2% of kiwi fruits, 1-2% of soybeans, 1-2% of cucumbers, 1-2% of carrots, 1-2% of lotus roots, 1-2% of pumpkins, 1-2% of potatoes, 1-2% of tomatoes, 1-2% of corns, 1-2% of mangosteens and 1-2% of ginger; and the auxiliary material comprises the following components in percentages by weight: 5-7% of brown sugar, 5-7% of honey, 6-9% of a bacterial enzyme and the balance of purified water. According to the natural fruit and vegetable enzyme compound beverage and the preparation method, relatively multiple nutrients are better involved; usage requirements of different demanders are met; and the operating requirements of the required enzyme of a user are met.

A method for improving the content of GABA (gamma-aminobutyric acid) in sprouted peanuts includes the following steps: selecting well grown peanuts, cleaning dust through clear water, washing the peanuts through distilled water and draining off water; soaking peanuts in a sodium hypochlorite solution for disinfection, cleaning the peanuts through distilled water, soaking the peanuts in water under a dark condition; performing intensive pulse light treatment to the soaked peanuts, cleaning the peanuts through distilled water, adding water the same equal quantity as the peanuts, and soaking the peanuts in water under a dark condition to enable the peanuts to sprout; spraying distilled water, until the sprout length is 10-25 mm obtaining sprouted peanuts, and performing enzyme deactivation; performing vacuum freeze drying and vacuum package, and storing the peanuts in a refrigeration storage at the temperature of 10 DEG C below. According to the invention, the intensive pulse light physical manner is adopted for treatment, the operation is simple, the enzyme activity is improved, protease and glutamic acid decarboxylase inside peanuts are activated to accelerate the proteolysis and enhance the growth vigor and stress resistance of peanut seeds, and the content of GABA in sprouted peanuts reaches 280 mg / 100g or above.

The invention relates to the technical field of cleaners, and particularly, relates to an enzyme stock solution and an environmental-protection enzyme, and preparation methods thereof. The preparation method of the enzyme stock solution comprises the steps of taking 1-1.5 parts by weight of brown sugar, 2-4 parts by weight of straw and 8-11 parts by weight of water, adjusting the pH value of a brown sugar, water and straw mixed solution to 4-6, then carrying out anaerobic fermentation, and filtering to obtain the product. The specific preparation method of the environmental-protection enzyme with the obtained enzyme stock solution comprises the steps: carrying out mixed fermentation of the enzyme stock solution with Chinese honeylocust fruit, or carrying out mixed fermentation of the enzyme stock solution and Chinese honeylocust fruit fermentation liquid. The preparation method of the enzyme stock solution turns the straw into treasure, and the obtained enzyme stock solution also has a good cleaning effect. According to the preparation method of the environmental-protection enzyme, the Chinese honeylocust fruit or the Chinese honeylocust fruit fermentation liquid are added in the enzyme stock solution, the anaerobic fermentation is carried out, the content of the enzyme in the environmental-protection enzyme is effectively increased, the dirt-removing power of the environmental-protection enzyme is enhanced, and the obtained environmental-protection enzyme is guaranteed to have good dirt-removing effect.

The invention relates to the technical field of crop planting, and discloses a plant fermentation extract for improving rice planting soil and a preparation method of the plant fermentation extract. The plant fermentation extract for improving the rice planting soil is characterized by being prepared from cortex lycii radicis, tricholoma matsutake, pollen, radix sophorae flavescentis, dried lumbricus, hirudin, liquorice, cyrtomium fortunei, rhizoma phragmitis, pericarpium citri reticulatae, bamboo leaves, shredded coconut stuffing, hippophae rhamnoides, radix bupleuri, echinopsis tubiflora flower, salicornia europaea, fritillary bulb,radix glehniae, fructus aurantii, fructus evodiae, lentils, astragalus, amomum tsao-ko, selenium yeast, iron enriched yeast, rice germ, hemp seed and red ginseng, adopted as raw materials, are mixed according to a certain weight ratio, and the mixture is fermented, extracted and ground into powder. The raw materials are easy to obtain, the preparation process is simple, the soil structure is effectively improved, the number and activity of effective microorganisms in soil are increased, the soil enzyme content is increased, rice growth, yield increaseand quality improvement are facilitated. The method is suitable for wide application and popularization.

The invention relates to the technical field of agricultural planting, and particularly discloses a plant fermentation extract for treating rice root rot and a preparation method thereof. The plant fermentation extract for treating rice root rot is prepared from following raw materials through fermenting, extracting and pulverizing, in parts by weight, 0.3 to 0.5 part of hirudin,20 to 25 parts ofcyrtomium fortunei; 30-32 parts of cortex lycii radicis, 2-3 parts of rhizoma phragmitis, 10-15 parts of bamboo leaf extract, 5-10 parts of pericarpium citri reticulatae, 1-3 parts of red ginseng, 15-20 parts of liquorice, 5-7 parts of pollen, 20-25 parts of radix bupleuri, 2-3 parts of shredded coconut stuffing, 3-5 parts of sea buckthorn, 0.1-0.2 part of echinopsis tubiflora flower, 0.1-0.15 part of zinc-enriched yeast, and 0.2 to 0.25 part of selenium yeast. The raw materials are easy to obtain, the preparation process is simple, soil flora is effectively improved, the number and activity of effective microorganisms in soil are increased, the soil enzyme content is increased, rice growth is facilitated, the yield is increased, and the plant fermentation extractis suitable for wide application and popularization.

The invention discloses a full-plant fruit / vegetable enzyme and a preparation method thereof. The preparation method mainly comprises the following steps: uniformly mixing hundreds of fresh fruits andvegetables and white sugar as raw materials in a certain mass ratio, implementing four inoculation processes, fermenting for more than 540 days, and gradually fermenting fruit and vegetable layers, thereby obtaining the full-plant fruit / vegetable enzyme. The enzyme prepared by using the method disclosed by the invention is free of additive or moisture, rich in trace element such as vitamins, amino acids and minerals essential for human bodies, easy to absorb, capable of improving digestion, promoting metabolism and improving human immunity, and applicable to both the young and the old.

The invention relates to the technical field of corn planting, and particularly discloses a plant fermentation extract for improving corn planting and a preparation method thereof. The plant fermentation extract is characterized in that the plant fermentation extract is prepared from, by weight, 3-4 parts of matsutake, 1-2 parts of radix ginseng, 10-15 parts of Sophora flavescens, 10-15 parts of danshen roots, 5-10 parts of corn stigma, 10-15 parts of seaweed, 3-5 parts of dried tangerine peel, 2-5 parts of eucommia ulmoides, 1-2 parts of cordyceps sinensis, 15-17 parts of radix bupleuri, 0.03-0.05 part of zinc-enriched yeast and 1-2 parts of royal jelly through fermentation, extraction and powder preparation. Raw materials are easy to obtain, the preparation process is simple, and the plant fermentation extract can effectively improve the soil structure, increase the number and activity of effective microorganisms in soil, improve the soil enzyme content, facilitate corn growth, yieldincrease and quality improvement, and is suitable for wide application and popularization.

The invention discloses helianthus tuberosus ferment as well as a preparation method, preparation and application thereof. The helianthus tuberosus ferment consists of the following raw materials in parts by weight: 60-80 parts of fresh helianthus tuberosus, and 20-40 parts of sugar. The fresh helianthus tuberosus is used as the raw materials, so that on one hand, the prepared helianthus tuberosus ferment has special color and smell and is good in mouth feel, and the fragrances lasts long; and on the other hand, the helianthus tuberosus ferment is rich in special functional components of inulin, polyphenol flavonoid and the like in the helianthus tuberosus, and nutrient components of various natural vitamins, minerals, trace elements, amino acids and the like. According to the preparation method of the helianthus tuberosus ferment, normal-temperature normal-pressure fermentation is adopted, and fermentation microorganisms come from preferable microorganisms in natural raw materials of the helianthus tuberosus, helianthus tuberosus tragon and the like, so that the helianthus tuberosus ferment is green and safe, notable in efficacy and strong in pertinence, has notable efficacy of improving flora of intestinal tracts of human bodies, resisting metabolic diseases of diabetes, high blood lipid and the like, eliminating free radicals, resisting oxidation, enhancing immunity, resisting tumors and the like, effectively strengthens metabolism, and achieves the purpose of strengthening bodies when being used for complementing ferment of the human bodies.

The invention discloses a secondary cordyceps militaris enzyme fermentation method and relates to the technical field of enzyme preparation. According to the method disclosed by the invention, yeast extract, glucose, coconut milk, condensed milk and potatoes are added in the secondary fermentation process and are substances loved by saccharomycetes and lactic acid bacteria, and the yeast extract, glucose, coconut milk, condensed milk and potatoes are actively selected by the saccharomycetes and lactic acid bacteria in the fermentation process, so that the problem that enzymes in fermentation liquor or enzymes in other raw materials can be solved. The obtained product has high enzyme content.

The invention relates to the technical field of crop planting, and particularly discloses a plant fermentation extract for improving potatoes and a preparation method of the plant fermentation extract. The plant fermentation extract for improving potato planting is characterized by being prepared by combining tricholoma matsutake, red ginseng, pollen, dendrobium, radix sophorae flavescentis, sea buckthorn, cyrtomium fortunei, dark plum, dried earthworm, salicornia europaea, dried lacquer, medicated leaven, cordyceps sinensis, bamboo leaves, oriental wormwood, hirudin, dried tangerine or orangepeel, liquorice, dandelion, selenium yeast and zinc-enriched yeast according to a certain weight ratio, and conducting fermentation, extraction and powdering. Raw materials are easily available, thepreparation process is simple, the soil structure is effectively improved, the number and activity of effective microorganisms in soil are increased, the soil enzyme content is increased, potato growth, yield increase and quality improvement are facilitated, and the plant fermentation extract is suitable for wide application and popularization.

The invention discloses an envelope production process for granzyme for a temperature-resistant feed. The process comprises the following steps of: mixing raw enzyme and a carrier, namely mixing the raw enzyme and the carrier to obtain paste, wherein the carrier consists of calcium carbonate, ethyl cellulose, corn starch and water, and the granzyme is prepared from the following raw materials in percentage by weight: 20 percent of calcium carbonate, 10 to 20 percent of ethyl cellulose, 20 to 30 percent of corn starch, 20 to 40 percent of raw enzyme and the balance of water; granulating; performing shot blasting; coating; and drying. By the process, the thermal stability, particularly the humidity resistance of the enzyme can be improved. Enteric polyacrylic resin latex is used as a coating material, so the use of an organic solvent can be avoided in the coating process, the production is safe, and three wastes are not generated.

The invention relates to the technical field of agricultural planting, and particularly discloses a plant fermentation extract for improving soybean planting soil and a preparation method of the plantfermentation extract. The plant fermentation extract for improving the soybean planting soil is characterized by being prepared by taking cape jasmine, tricholoma matsutake, dried lumbricus, hirudin,cordyceps sinensis, pollen, radix sophorae flavescentis, liquorice, hippophae rhamnoides, Chinese wolfberry, pericarpium citri reticulatae, salicornia europaea, cyrtomium fortunei, radix stemonae, akebiaquinata, iron-enriched yeast, selenium yeast, oyster, magnesium yeast and soybean germ as raw materials. Those raw materials are mixed according to certain weight proportion. The plant fermentation extract is made through fermention, extraction, and flour milling process. Raw materials are easy to obtain, the preparation process is simple, the soil structure is effectively improved, the numberand activity of effective microorganisms in soil are increased, the soil enzyme content is increased, soybean growth, yield increase and quality improvement are facilitated, and the method is suitable for wide application and popularization.

The invention relates to a method for efficiently synthesizing L-theanine by using gamma-glutamyl transpeptidase produced by Escherichia coli. The method comprises the following steps of: a) filtering a raw fermentation liquor of Escherichia coli by using an ultrafiltration membrane with the aperture of 0.001-0.01um; b) adding an ethylamine solution into an enzyme and bacterium trapped fluid to regulate the pH to be 10-11, and standing for 2 to 3 hours to obtain an enzyme concentrated solution; and c) adding the enzyme concentrated solution obtained in the step b) into an aqueous solution containing L-glutamine to obtain an enzyme reaction system, adding an ethylamine solution into the enzyme reaction system to control the pH of the enzyme reaction system to be 9-10.5, and controlling the enzymatic conversion temperature to be 30-35DEG C. The method is easy to implement and low in time and energy consumption, avoids the introduction of impurities and the deactivation of gamma-glutamyl transpeptidase, avoids the corrosion of a reactor due to the introduction of hydrochloric acid, improves the yield and purity of the L-theanine and reduces cost.

The invention discloses a composite forage capable of improving broiler chicken immunity. The composite forage capable of improving broiler chicken immunity comprises, by weight, 90 to 110 parts of amain material, 10 to 18 parts of brewer's grain, 12 to 25 parts of peanut meal, 10 to 19 parts of fly maggot protein powder, 12 to 22 parts of fermented sesame meal, 1 to 2 parts of wood dust, 0.2 to1.1 parts of corn cob powder, 1 to 2 parts of aloe powder, 0.4 to 1 part of royal jelly, 0.2 to 0.5 part of composite amino acid, 0.05 to 0.3 part of phytase, and 0.01 to 0.08 part of trace element. Modified polyfructosan is prepared through following steps: carbon nanotube is added into hydrochloric acid, heating backflow, filtering, washing, and drying are carried out, polyfructosan and urea areadded for uniform mixing, heating, thermal insulation, filtering, and washing are carried out, an ethanol aqueous solution and lanthanum oxide are added, heating is carried out, an obtained product is allowed to stand, and stirring, filtering, roasting, and crushing are carried out so as to obtain the modified polyfructosan.

The invention relates to the technical field of enzyme immobilization, in particular to an immobilized enzymatic-conversion large-flowered skullcap root and application thereof to acetaminophen damagerepairing. The preparation method comprises the following steps: mixing an enzyme solution with a pretreated carrier material and then carrying out absorption treatment; carrying out cross-linking treatment on the processed mixture and across-linking agent; and carrying out washing to obtain an immobilized enzyme. The carrier material is selected from macroporous resin. The enzyme contains pectinase. The immobilized enzyme provided by the invention has high enzyme content and stability and can be repeatedly recycled; and the production cost of enzymatic reaction can be lowered. The immobilized enzyme can be applied to biotransformation; the baicalin can be treated under mild reaction conditions to obtain a single product baicalein without any byproduct; and the product is easy to separate. The conversion product obtained by utilizing the immobilized enzyme has more remarkable protection and repair effects on the damage of acetaminophen to immune cells.

The invention discloses a sturgeon fry bait ecologic cultivation method. The sturgeon fry bait ecologic cultivation method comprises steps of constructing a bait pool, performing disinfection on the bait pool, adopting exclusive guano to perform water fertilization five days after disinfection, wherein an application amount of the exclusive guano is 8-12kg / 667m3, draining water in the bait pool every march, uniformly distributing water earthworm larvae on the bottom of the bait pool, putting water to cover the water earthworm larvae on the bottom of the bait pool, wherein the water depth of the bait pool is 3-6cm, the water depth in the bait pool during the day is 3-4cm and the water depth of the bait pool during the night is 5-6cm, performing water quality regulation, with regulation frequency being once in every 14-16 days, and capturing. The sturgeon fry bait ecologic cultivation method can promote the sturgeon fries to feed, improves growing speed of the sturgeon fries, improves immunity of the water earthworm, reduces pathogens carried by the living bodies of the sturgeon fries and reduces likelihood of having diseases.

The invention discloses a method for extracting enzyme from animal placentae. The method comprises steps as follows: 1) the animal placentae are taken as raw materials, the raw materials are pretreated, crushed, subjected to ultrasonic cell wall breaking twice and homogenized with water, and a mixed solution is obtained; 2) the mixed solution obtained in the step 1) is centrifuged, and a cell stock solution is obtained; 3) the cell stock solution obtained in the step 2) is adsorbed by ion exchange resin, a product is eluted, filtered, dialyzed and lyophilized, and an enzyme product is obtained. The enzyme product is high in yield and good in stability, and enzyme content of the enzyme product is high.

The invention discloses a microbial fertilizer containing biological enzyme amino acid, and belongs to the technical field of biological fertilizers. The microbial fertilizer containing biological enzyme amino acid is prepared from the following raw materials in parts by weight: 10-40 parts of animal waste, 1-15 parts of furfural residue, 10-40 parts of vegetable protein, 10-40 parts of biological enzyme-containing material, 0.1-5 parts of animal crushed bone, 0.1-3 parts of medium trace elements, 1-5 parts of enzyme bacteria, 0.3-3 parts of enzymolysis bacteria, 1-6 parts of microbial agent and 15-60 parts of water. The invention also provides a preparation method of the biological enzyme-containing amino acid microbial fertilizer. The microbial fertilizer disclosed by the invention has the effects of balanced nutrition, strong fertilizer efficiency and capabilities of improving crop quality, repairing soil and reducing production cost.

The invention provides a traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from two or a combination of more of the following raw materials: rhizoma dioscoreae, arillus longan, folium mori, exocarpium citri rubrum, rhizoma polygonati odorati, bulbus fritillariae cirrhosae, bighead atractylodes rhizome, semen lablab album and lotus leaf. The invention also provides a pharmaceutical preparation containing the traditional Chinese medicine composition, and application of the traditional Chinese medicine composition in preparation of the pharmaceutical preparation for preventing or relieving lung injury caused by inhalable particles. The traditional Chinese medicine composition provided by the invention increases the contents of superoxide dismutase and glutathione peroxidase by reducing the accumulation of active oxygen, malondialdehyde, interleukin 6, interleukin-8 and a tumor necrosis factor, thereby improving the anti-oxidation and anti-inflammatory abilities of a human body, enhancing the body immunity and further achieving the purpose of resisting the damage, caused by the inhalable particles, to the human body.

The present invention relates to a production process for ginkgo green tea, including the following steps: gathering ginkgo leaves between Qingming and the beginning of summer; cleaning the leaves with water and drying in the sun; spraying an edible alkali powder solution; sprinkling green onion powder and garlic powder, stirring uniformly, feeding into a dryer for drying; sprinkling malt powder, stirring uniformly, and feeding into the dryer again for drying; and sprinkling honeysuckle pollens and murraya paniculata pollens, stirring, cooling for vacuum packaging to obtain the ginkgo green tea. According to the method, a multi-stage drying process is adopted to prepared the ginkgo green tea; and by adding the green onion powder, the garlic powder, the honeysuckle pollens and the murraya paniculata pollens in ginkgo leaves, the flavor, color, leaf shape, and taste of the gingko green tea are ensured, and the gingko green tea has bactericidal, cold-preventing, chest-opening and qi smoothing, stomach-tonifying and other effects. The process is simple and convenient, and the cost is low.

The invention discloses a method for extracting enzymes in animal placenta, which comprises the following steps: 1) using animal placenta as a raw material, undergoing pretreatment, crushing, ultrasonic wall breaking twice, adding water and homogenizing to obtain a mixed solution; 2) the The mixed solution obtained in step 1) is centrifuged to obtain a cell stock solution; 3) the cell stock solution obtained in step 2) is adsorbed by an ion exchange resin, eluted, filtered and dialyzed, and freeze-dried to obtain an enzyme product; the enzyme The product yield is high and the stability is good, and the enzyme content in the enzyme product is high.

A process for preparing calf rennase from the rennet stomach of calf as material includes such steps as low-temp. inducing, extracting with aqueous solution of NaCl and alcohol, and purifying with ion exchange column and molecular-sieve gel to obtain the partly purified rennase. It has the most adaptive temp. of 50 deg.C, the most adaptive pH value of 5.8, and stable enzyme activity in pH 3.0-6.0 range. Its advantages are low cost, easily available raw materials and high recovery rate of enzyme content and activity.

The invention discloses an industrial preparation method of a dual-functional radish enzyme that is provided with the activity of both chitinase and muramidase and comprises the following steps: firstly raw material is washed and cut, low-temperature induction is implemented to produce the enzyme, homogenate is agitated with a homogenate liquid, a concentrated solution of the dual-functional radish enzyme is obtained through steps of centrifugation, filtering and hyperfiltration and the like, and a protecting agent and a stabilizing agent are respectively added into the concentrated solution,thus obtaining the liquid dual-functional radish enzyme or the dry dual-functional radish enzyme powder. The industrial preparation method of the dual-functional radish enzyme has the following advantages: 1. low-temperature lighting induction technique is adopted, thus raising the raw enzyme content by 2 to 3 times; 2. specific activity stabilizing agent and protecting agent of the dual-functional radish enzyme are developed, thus keeping the activity of the dual-functional radish enzyme basically unchanged in two years at room temperature and reaching the activity of over 97 percent of the raw enzyme; 3. the raw material has wide source and low price, is not affected by factors including season and weather and the like, and can be planted in all seasons; 4. the preparation method has simple technique and low price.

A method for increasing the content of γ-aminobutyric acid in germinated peanuts. Select well-grown peanuts, wash away the dust with clear water, then wash with distilled water, and drain; soak and disinfect with sodium hypochlorite solution, wash with distilled water, and place in a safe place. Under light conditions, soak in water; after the soaked peanuts are pulsed with strong light, wash them with distilled water, add water of the same quality as the peanuts, soak them in dark conditions to make them germinate; spray distilled water, and wait for the peanuts to germinate The sprouts grow to 10 mm to 25 mm to obtain germinated peanuts, and the enzymes are inactivated; they are vacuum freeze-dried and vacuum-packed, and stored in a cold storage below 10°C. Using pulsed strong light physical treatment, easy to operate, can increase the activity of enzymes, activate protease and glutamic acid decarboxylase in peanuts, accelerate protein decomposition, enhance the growth and stress resistance of peanut seeds, and the GABA content of germinated peanuts can reach More than 280mg / 100g.

The present invention relates to a production process for ginkgo green tea, including the following steps: gathering ginkgo leaves between Qingming and the beginning of summer; cleaning the leaves with water and drying in the sun; spraying an edible alkali powder solution; sprinkling green onion powder and garlic powder, stirring uniformly, feeding into a dryer for drying; sprinkling malt powder, stirring uniformly, and feeding into the dryer again for drying; and sprinkling honeysuckle pollens and murraya paniculata pollens, stirring, cooling for vacuum packaging to obtain the ginkgo green tea. According to the method, a multi-stage drying process is adopted to prepared the ginkgo green tea; and by adding the green onion powder, the garlic powder, the honeysuckle pollens and the murraya paniculata pollens in ginkgo leaves, the flavor, color, leaf shape, and taste of the gingko green tea are ensured, and the gingko green tea has bactericidal, cold-preventing, chest-opening and qi smoothing, stomach-tonifying and other effects. The process is simple and convenient, and the cost is low.

The invention discloses a process for preparing a tea enzyme liquid from fresh tea leaves and belongs to the field of preparation of tea source drinks. The fresh tea leaves are taken, cleaned, rolled and mixed with a yeast impregnating solution, 9% (V / V) rice vinegar and brown sugar are added, the mixture is uniformly stirred, fermented for more than one year in a shady and dry place, centrifuged and filtered, and the tea enzyme liquid is obtained. The fresh tea leaves are leached in water at the normal temperature, saccharomycetes, the rice vinegar and the brown sugar are combined for fermentation, and the tea enzyme liquid is prepared, doesn't lose nutritional ingredients and is healthier, safer and more efficient.

The invention relates to the technical field of cleaners, and particularly, relates to an enzyme stock solution and an environmental-protection enzyme, and preparation methods thereof. The preparation method of the enzyme stock solution comprises the steps of taking 1-1.5 parts by weight of brown sugar, 2-4 parts by weight of straw and 8-11 parts by weight of water, adjusting the pH value of a brown sugar, water and straw mixed solution to 4-6, then carrying out anaerobic fermentation, and filtering to obtain the product. The specific preparation method of the environmental-protection enzyme with the obtained enzyme stock solution comprises the steps: carrying out mixed fermentation of the enzyme stock solution with Chinese honeylocust fruit, or carrying out mixed fermentation of the enzyme stock solution and Chinese honeylocust fruit fermentation liquid. The preparation method of the enzyme stock solution turns the straw into treasure, and the obtained enzyme stock solution also has a good cleaning effect. According to the preparation method of the environmental-protection enzyme, the Chinese honeylocust fruit or the Chinese honeylocust fruit fermentation liquid are added in the enzyme stock solution, the anaerobic fermentation is carried out, the content of the enzyme in the environmental-protection enzyme is effectively increased, the dirt-removing power of the environmental-protection enzyme is enhanced, and the obtained environmental-protection enzyme is guaranteed to have good dirt-removing effect.