36results about How to "Increased enzyme content" patented technology

The invention relates to a method for efficiently synthesizing L-theanine by using gamma-glutamyl transpeptidase produced by Escherichia coli. The method comprises the following steps of: a) filtering a raw fermentation liquor of Escherichia coli by using an ultrafiltration membrane with the aperture of 0.001-0.01um; b) adding an ethylamine solution into an enzyme and bacterium trapped fluid to regulate the pH to be 10-11, and standing for 2 to 3 hours to obtain an enzyme concentrated solution; and c) adding the enzyme concentrated solution obtained in the step b) into an aqueous solution containing L-glutamine to obtain an enzyme reaction system, adding an ethylamine solution into the enzyme reaction system to control the pH of the enzyme reaction system to be 9-10.5, and controlling the enzymatic conversion temperature to be 30-35DEG C. The method is easy to implement and low in time and energy consumption, avoids the introduction of impurities and the deactivation of gamma-glutamyl transpeptidase, avoids the corrosion of a reactor due to the introduction of hydrochloric acid, improves the yield and purity of the L-theanine and reduces cost.

The invention discloses an envelope production process for granzyme for a temperature-resistant feed. The process comprises the following steps of: mixing raw enzyme and a carrier, namely mixing the raw enzyme and the carrier to obtain paste, wherein the carrier consists of calcium carbonate, ethyl cellulose, corn starch and water, and the granzyme is prepared from the following raw materials in percentage by weight: 20 percent of calcium carbonate, 10 to 20 percent of ethyl cellulose, 20 to 30 percent of corn starch, 20 to 40 percent of raw enzyme and the balance of water; granulating; performing shot blasting; coating; and drying. By the process, the thermal stability, particularly the humidity resistance of the enzyme can be improved. Enteric polyacrylic resin latex is used as a coating material, so the use of an organic solvent can be avoided in the coating process, the production is safe, and three wastes are not generated.

The invention discloses a method for extracting enzymes in animal placenta, which comprises the following steps: 1) using animal placenta as a raw material, undergoing pretreatment, crushing, ultrasonic wall breaking twice, adding water and homogenizing to obtain a mixed solution; 2) the The mixed solution obtained in step 1) is centrifuged to obtain a cell stock solution; 3) the cell stock solution obtained in step 2) is adsorbed by an ion exchange resin, eluted, filtered and dialyzed, and freeze-dried to obtain an enzyme product; the enzyme The product yield is high and the stability is good, and the enzyme content in the enzyme product is high.

A method for increasing the content of γ-aminobutyric acid in germinated peanuts. Select well-grown peanuts, wash away the dust with clear water, then wash with distilled water, and drain; soak and disinfect with sodium hypochlorite solution, wash with distilled water, and place in a safe place. Under light conditions, soak in water; after the soaked peanuts are pulsed with strong light, wash them with distilled water, add water of the same quality as the peanuts, soak them in dark conditions to make them germinate; spray distilled water, and wait for the peanuts to germinate The sprouts grow to 10 mm to 25 mm to obtain germinated peanuts, and the enzymes are inactivated; they are vacuum freeze-dried and vacuum-packed, and stored in a cold storage below 10°C. Using pulsed strong light physical treatment, easy to operate, can increase the activity of enzymes, activate protease and glutamic acid decarboxylase in peanuts, accelerate protein decomposition, enhance the growth and stress resistance of peanut seeds, and the GABA content of germinated peanuts can reach More than 280mg / 100g.

The present invention relates to a production process for ginkgo green tea, including the following steps: gathering ginkgo leaves between Qingming and the beginning of summer; cleaning the leaves with water and drying in the sun; spraying an edible alkali powder solution; sprinkling green onion powder and garlic powder, stirring uniformly, feeding into a dryer for drying; sprinkling malt powder, stirring uniformly, and feeding into the dryer again for drying; and sprinkling honeysuckle pollens and murraya paniculata pollens, stirring, cooling for vacuum packaging to obtain the ginkgo green tea. According to the method, a multi-stage drying process is adopted to prepared the ginkgo green tea; and by adding the green onion powder, the garlic powder, the honeysuckle pollens and the murraya paniculata pollens in ginkgo leaves, the flavor, color, leaf shape, and taste of the gingko green tea are ensured, and the gingko green tea has bactericidal, cold-preventing, chest-opening and qi smoothing, stomach-tonifying and other effects. The process is simple and convenient, and the cost is low.

The invention discloses a process for preparing a tea enzyme liquid from fresh tea leaves and belongs to the field of preparation of tea source drinks. The fresh tea leaves are taken, cleaned, rolled and mixed with a yeast impregnating solution, 9% (V / V) rice vinegar and brown sugar are added, the mixture is uniformly stirred, fermented for more than one year in a shady and dry place, centrifuged and filtered, and the tea enzyme liquid is obtained. The fresh tea leaves are leached in water at the normal temperature, saccharomycetes, the rice vinegar and the brown sugar are combined for fermentation, and the tea enzyme liquid is prepared, doesn't lose nutritional ingredients and is healthier, safer and more efficient.

The invention relates to the technical field of cleaners, and particularly, relates to an enzyme stock solution and an environmental-protection enzyme, and preparation methods thereof. The preparation method of the enzyme stock solution comprises the steps of taking 1-1.5 parts by weight of brown sugar, 2-4 parts by weight of straw and 8-11 parts by weight of water, adjusting the pH value of a brown sugar, water and straw mixed solution to 4-6, then carrying out anaerobic fermentation, and filtering to obtain the product. The specific preparation method of the environmental-protection enzyme with the obtained enzyme stock solution comprises the steps: carrying out mixed fermentation of the enzyme stock solution with Chinese honeylocust fruit, or carrying out mixed fermentation of the enzyme stock solution and Chinese honeylocust fruit fermentation liquid. The preparation method of the enzyme stock solution turns the straw into treasure, and the obtained enzyme stock solution also has a good cleaning effect. According to the preparation method of the environmental-protection enzyme, the Chinese honeylocust fruit or the Chinese honeylocust fruit fermentation liquid are added in the enzyme stock solution, the anaerobic fermentation is carried out, the content of the enzyme in the environmental-protection enzyme is effectively increased, the dirt-removing power of the environmental-protection enzyme is enhanced, and the obtained environmental-protection enzyme is guaranteed to have good dirt-removing effect.