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Method for improving content of GABA (gamma-aminobutyric acid) in sprouted peanuts

A technology for germinating peanuts and aminobutyric acid, which is applied in the fields of seed and rhizome treatment, agriculture, application, etc., and can solve the problem that the increase of γ-aminobutyric acid content cannot be stably controlled.

Active Publication Date: 2015-07-29
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that when plants are subjected to adversity stresses such as hypoxia, hypoxia, cold shock, heat shock, salt stress, and mechanical stimulation, the content of γ-aminobutyric acid (GABA) in plants will increase to a certain extent, but γ-aminobutyric acid The increase in content cannot be stably controlled

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1

[0021] 1.1. Pre-treatment of peanuts

[0022] Remove impurities, moldy grains and peanuts with poor maturity in peanuts, select well-grown peanuts, wash away the dust with tap water, then wash with distilled water, drain; soak in 0.8% sodium hypochlorite solution for 10 minutes for disinfection Afterwards, wash with distilled water for 3 times, put it under the condition of 28°C and avoid light, soak in water for 8h;

[0023] 1.2. Peanut pulsed strong light treatment

[0024] Open the pulsed strong light test cabinet, and after running for 10 minutes, the instrument will start stably. Set the pulse parameters according to the experimental conditions. The pulse flash energy is 300J, the pulse distance is 11cm, the frequency is 1 time / s, and the time is 30s. Evenly distribute in the center of the sterile quartz plate directly under the lamp tube in the pulsed strong light experimental cabinet, close the cabinet door, and start the pulsed strong light treatme...

Embodiment 2

[0035] 1.1. Pre-treatment of peanuts

[0036] Remove impurities, moldy grains and peanuts with poor maturity in peanuts, select well-grown peanuts, wash away the dust with tap water, then wash with distilled water, drain; soak in 1.2% sodium hypochlorite solution for 15 minutes for disinfection Finally, wash it with distilled water for 5 times, place it in the dark at 32°C, and soak it in water for 12 hours;

[0037] 1.2. Peanut pulsed strong light treatment

[0038] Open the pulsed strong light test cabinet, and after running for 10 minutes, the instrument keeps starting steadily. Set the pulse parameters according to the experimental conditions. The pulse flash energy is 100J, the pulse distance is 8cm, the frequency is 4 times / s, and the time is 3min. Evenly distribute in the center of the sterile quartz plate directly under the lamp tube in the pulsed strong light experimental cabinet, close the cabinet door, and start the pulsed strong light treatment;

[0039] 1.3 Germ...

Embodiment 3

[0044] 1.1. Pre-treatment of peanuts

[0045] Remove impurities, moldy grains and poorly mature peanuts from peanuts, select well-grown peanuts, wash off the dust with tap water, then wash with distilled water, and drain; soak in 1% sodium hypochlorite solution for 12 minutes for disinfection Afterwards, wash with distilled water for 4 times, place in 30°C and avoid light, and soak in water for 10 minutes;

[0046] 1.2. Peanut pulsed strong light treatment

[0047]Open the pulsed strong light test cabinet, and after running for 10 minutes, the instrument keeps starting steadily. Set the pulse parameters according to the experimental conditions. The pulse flash energy is 500J, the pulse distance is 13cm, the frequency is 2 times / s, and the time is 1min. Evenly distribute in the center of the sterile quartz plate directly under the lamp tube in the pulsed strong light experimental cabinet, close the cabinet door, and start the pulsed strong light treatment;

[0048] 1.3 Germin...

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Abstract

A method for improving the content of GABA (gamma-aminobutyric acid) in sprouted peanuts includes the following steps: selecting well grown peanuts, cleaning dust through clear water, washing the peanuts through distilled water and draining off water; soaking peanuts in a sodium hypochlorite solution for disinfection, cleaning the peanuts through distilled water, soaking the peanuts in water under a dark condition; performing intensive pulse light treatment to the soaked peanuts, cleaning the peanuts through distilled water, adding water the same equal quantity as the peanuts, and soaking the peanuts in water under a dark condition to enable the peanuts to sprout; spraying distilled water, until the sprout length is 10-25 mm obtaining sprouted peanuts, and performing enzyme deactivation; performing vacuum freeze drying and vacuum package, and storing the peanuts in a refrigeration storage at the temperature of 10 DEG C below. According to the invention, the intensive pulse light physical manner is adopted for treatment, the operation is simple, the enzyme activity is improved, protease and glutamic acid decarboxylase inside peanuts are activated to accelerate the proteolysis and enhance the growth vigor and stress resistance of peanut seeds, and the content of GABA in sprouted peanuts reaches 280 mg / 100g or above.

Description

technical field [0001] The invention relates to a method for increasing the content of gamma-aminobutyric acid in germinated peanuts, in particular to a method for increasing the content of gamma-aminobutyric acid in germinated peanuts by using pulsed strong light. Background technique [0002] When plant seeds are exposed to adversities such as drought and flood, low temperature, hypoxia, heat shock, cold shock, and machinery, they often suffer from different degrees of damage, which leads to changes in plant physiological and biochemical processes, and then affects the production of nutrients. Under adversity conditions, the plant body will be harmed, such as the reduction of water absorption capacity of the plant body, the lack of water in the body, and the increase of growth inhibitory substances. [0003] The metabolic intensity in plant seeds is closely related to their stress resistance. Appropriate intensity and physical energy can accelerate the metabolic process an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
Inventor 马涛娄山王晓琳陈妍婕王勃
Owner BOHAI UNIV
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