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Method for extracting enzyme from animal placentae

A technology for animal placenta and cell stock solution, which is applied in the fields of medicine and health care products, can solve the problem that the extraction method is not suitable for extraction, etc., and achieve the effects of sufficient separation, improved release degree, and increased enzyme content.

Active Publication Date: 2018-02-09
TIBET LIANGDI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the commonly used methods for extracting active ingredients in animal placenta on the market are methods for extracting animal element, which is not suitable for extracting enzymes in animal placenta.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for extracting enzymes in animal placenta, comprising the steps of:

[0042] 1) Using animal placenta as raw material, after cleaning, removing impurities, and cutting into 0.5-1.5cm 3 fragments, pulverized, ultrasonically broken twice, and homogenized with water to obtain a mixed solution;

[0043] 2) The mixed solution obtained in the step 1) is centrifuged to obtain the cell stock solution;

[0044] 3) The cell stock solution obtained in step 2) is adjusted to a pH value of 4-6.5, and after being adsorbed by a weakly acidic cation exchange resin, it is eluted with ammonium sulfate at a flow rate of 2-4 mL / min, and the molecular cut-off flow rate is 3000 The ~10000Da dialysis membrane is subjected to filtration and dialysis treatment, freeze-dried to obtain an enzyme product; the enzyme product includes: alkaline phosphatase, lactate dehydrogenase and superoxide dismutase.

[0045] Wherein, the conditions for the two times of ultrasonic wall breaking are: u...

Embodiment 2

[0048] The difference between the present embodiment and the embodiment 1 is only that the ultrasonic wall breaking conditions of the two times are: the ultrasonic power is 200w, the frequency is 25Hz, and the ultrasonic time is 15-20min.

[0049] The yield of the enzyme product in this embodiment is 3.10%, and the enzyme content in the enzyme product is 66%.

Embodiment 3

[0051] The difference between this embodiment and Embodiment 1 is that the ultrasonic wall breaking conditions of the two times are: the ultrasonic power is 350w, the frequency is 45Hz, and the ultrasonic time is 15-20min.

[0052] The yield of the enzyme product in this embodiment is 3.25%, and the enzyme content in the enzyme product is 64%.

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Abstract

The invention discloses a method for extracting enzyme from animal placentae. The method comprises steps as follows: 1) the animal placentae are taken as raw materials, the raw materials are pretreated, crushed, subjected to ultrasonic cell wall breaking twice and homogenized with water, and a mixed solution is obtained; 2) the mixed solution obtained in the step 1) is centrifuged, and a cell stock solution is obtained; 3) the cell stock solution obtained in the step 2) is adsorbed by ion exchange resin, a product is eluted, filtered, dialyzed and lyophilized, and an enzyme product is obtained. The enzyme product is high in yield and good in stability, and enzyme content of the enzyme product is high.

Description

technical field [0001] The invention relates to the fields of medicine and health products, in particular to a method for extracting enzymes in animal placenta. Background technique [0002] Animal placenta, also known as afterbirth, is a transitional organ for exchanging substances between mother and child formed by embryonic membrane and maternal endometrium during pregnancy in mammals. Animal placenta contains a variety of enzymes with application value. However, most of the methods commonly used in the market for extracting active ingredients in animal placenta are animal element extracts, and the extraction method is not suitable for extracting enzymes in animal placenta. Contents of the invention [0003] In view of this, the present application provides a method for extracting enzymes in animal placenta, the enzyme product has high yield and good stability, and the enzyme product has high enzyme content. [0004] In order to solve the above technical problems, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12N9/04C12N9/02
CPCC12N9/0006C12N9/0089C12N9/16C12Y115/01001C12Y301/03001
Inventor 段鑫向孝成
Owner TIBET LIANGDI BIOTECH CO LTD
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