The invention relates to a preparation method of a
liposome and the
liposome prepared by the preparation method. The preparation method of the
liposome comprises the following steps: (1) respectively preparing a lipoid molecule solution and a guest molecule solution; and (2) mixing the mixed lipoid molecule solution prepared in step (1) with a host molecule solution, so as to obtain the liposome. The liposome obtained by the preparation method is a single-compartment liposome of which the particle size is distributed in the range of 15-100nm, especially, in the range of 20-80nm. The invention also provides a preparation method of a
drug-carrying liposome as well as the
drug-carrying liposome obtained by the preparation method. The liposome or the
drug-carrying liposome prepared by the preparation method is small in particle size and narrow in distribution, so that the liposome drug can be more rapidly administered to the
lesion to improve
curative effect; moreover, the micro-particle-size liposome prepared by the preparation method is capable of reaching the inside of the
tumor tissue where the regular liposome cannot reach. Furthermore, the in-vivo
transport time of the liposome drug is shortened by the rapid administration of the liposome drug, so that the degradation loss of the liposome drug by the
organism enzyme system and the
immune system is further reduced so as to reduce the toxic and side effects.