The invention discloses equipment for detecting the sealing performance of medical protective clothing. The device specifically comprises a shell, a detection bin, protective clothing, a fixing hoop and a protective clothing fixing cylinder, wherein a door body is hinged to the middle position of one end of the shell, a control panel is arranged on the shell above the door body, a detection bin isarranged in the middle of the interior of the shell, a protective garment is fixed in the middle of the interior of the detection bin, a gas circulation cavity is formed in the side, close to the detection bin, between the shell and the detection bin, and a liquid circulation cavity is formed in the side, close to the shell, between the shell and the detection bin. The medical protective clothingsealing performance detection equipment is provided with the protective clothing fixing cylinder and a fixing hoop, cuffs, trouser legs and a collar of the protective clothing can be sealed, subsequent sealing performance detection is facilitated, and compared with traditional dummy simulated wearing, the fixing mode is more convenient, the tedious wearing and taking-off process is avoided, and detection efficiency is improved.