The invention discloses anti-hair loss shampoo. The anti-hair loss shampoo is prepared from the following raw materials: 3-7 parts of polygonati rhizoma, 4-6 parts of zingiberis rhizoma recens, 4-6 parts of platycladi cacumen, 8-12 parts of gleditsiae sinensis fructus, 9-11 parts of semen sapindi, 1-5 parts of polygoni multiflori radix, 2-4 parts of angelicae sinensis radix, 1 part of folium mori,2-4 parts of herba leonuri and 1-5 parts of menthae haplocalycis herba. The shampoo has the effects of caring hair and scalp, regulating water-oil balance of the hair and removing dandruff and relieving itching, and can keep the scalp healthy and the hair beautiful after being used for a long time; and the shampoo takes Chinese herbal medicines as raw materials, the Chinese herbal medicines are absorbed by scalp, hair follicle and hair to achieve the effects of cleaning, diminishing inflammation, sterilizing of skin surface, dispersing blood stasis, promoting blood circulation, tonifying qi and blood, nourishing liver and kidney and dredging meridians, the effects of overall comprehensive conditioning and local both exterior and interior treatment are achieved, and the effects of enablingscalp, hair follicle and hair to be healthy and enabling hair to grow luxuriant, black and glossy are achieved.