40 results about "Telomeric dna" patented technology

The invention discloses an optical method for detecting the four-chain-motif conformation of DNA, comprising the following steps: 1) the cationic water-soluble conjugated polymer solution with equivalent charge amount is added to DNA solution; 2) and then colorimetric and / or fluorescence detection are / is carried out to the DNA solution, and finally the C-rich sequence conformation of the DNA to be detected, such as human telomere DNA, can be judged according to the color change of the solution, fluorescence spectra or UV-visible absorption spectrum of the solution. The invention has simple method and high selectivity and sensitivity, and has important significance in biological detection, early diagnosis and treatment of diseases.

The invention belongs to the fields of medicaments and chemical industry, and discloses a peptide derivative of benzfuran quinoline and a preparation method thereof and an application thereof as an antitumor medicament. The structural formula of the peptide derivative of benzfuran quinoline is shown in the specifications, wherein R is a peptide chain, i.e., R, GG, GR, GK, GH, GF, HR, KR, FR, GGR, GFR, GFRK, GFHR or GGGR; R represents arginine; G represents glycine; K represents lysine; H represents histidine; and F represents phenyl alanine. The invention further discloses a preparation method of the peptide derivative of benzfuran quinoline and an application of the peptide derivative as an anticancer medicament. As proved by an experiment, the peptide derivative of benzfuran quinoline related to the invention has a very strong inhibiting effect on the expression of telomere DNAs (Deoxyribose Nucleic Acids) and proto-oncogene DNAs such as c-myc and the like, a remarkable inhibiting effect on various cancer cell lines, low toxicity on normal cells and a wide application space on the preparation of an anticancer medicament.

The invention belongs to the fields of drugs and chemical industry, and discloses a quinazoline derivative and a preparation method thereof and an application of the quinazoline derivative used as an anticancer drug. The structural formula of the quinazoline derivative is shown in the specifications, wherein R1 is NH(CH2)mNR5 or NH(CH2)m-Ar; R2 is NHCO(CH2)n NR5, NHCO(CH2)n-Ar, NHCO(CH2)nNH(CH2)nNR5 or NHCO(CH2)nNH(CH2)n-Ar; R3 is F, Cl, Br, I, H, CH3, SO2CH3 or OCH3; R4 is H, NHCO(CH2)nNR5, NHCO(CH2)n-Ar, NHCO(CH2)nNH(CH2)nNR5 or NHCO(CH2)nNH(CH2)n-Ar; -Ar represents various aromatic rings comprising various aromatic heterocyclic rings; m is equal to 2, 3 or 4; n is equal to 1, 2, 3, 4 or 5; and R5 represents alkyl of C1-6, cycloalkyl of C3-6, piperidyl, morpholinyl, piperazinyl or quinoxalinyl. The invention simultaneously discloses the preparation method of the quinazoline derivative and the application of the quinazoline derivative used as the anticancer drug. The experiment proves that the quinazoline derivative of the invention has strong inhibiting effect on telomere DNA expression, has obvious inhibiting effect on various kinds of cancer cell strains, has low toxicity to normal cells, and has wide application prospects in preparation of anticancer drugs.

The invention belongs to the fields of medicament and chemical industry, and discloses a carbazole derivative, a preparation method of the carbazole derivative, and an application of the carbazole derivative serving as an anticancer drug. The structural formula of the carbazole derivative is shown in the specification, wherein R1 is piperidyl, morpholinyl, piperazinyl or Bielin group; n is 1, 2 or 3; and R2 is pyrazolyl or pyridyl. At the same time, the invention discloses a preparation method of the carbazole derivative and an application of the carbazole derivative serving as the anticancer drug. The carbazole derivative has strong inhibiting action on the expression of telomere DNA (deoxyribonucleic acid) and DNA of c-myc and other proto-oncogene, has significant inhibiting action on multiple cancer cell strains, has low toxicity to normal cells, and has broad application space in preparation of anticancer drugs.

The invention discloses a Chinese herbal medicine compound preparation for treating liver cancer and a production method thereof. The Chinese herbal medicine compound preparation is mainly prepared from the following main Chinese herbal medicine raw materials in parts by weight: 1-15 parts of herba acalypha australis, 5-30 parts of herba sarcandrae, 5-15 parts of tape grass and 5-15 parts of radix scrophulariae. The production method of the Chinese herbal medicine compound preparation is characterized in that the medicines are prepared into an oral solution, a capsule, a tablet, a granule and the like through modern pharmaceutical production techniques such as water extraction, alcohol precipitation, concentration and powder preparation. The traditional Chinese medicine preparation has the effects of dispelling wind, removing meridian obstruction, promoting blood circulation to remove blood stasis and the like, also plays an anticancer effect, and can be used for slowing down and gradually shortening DNA (Deoxyribonucleic Acid) replication of telomeres of cancer cells by inhibiting the telomerase activity of the cancer cells so that the growth of the cancer cells is slowed down and finally the cancer cells head towards apoptosis, so the Chinese herbal medicine compound preparation is suitable for treating the liver cancer, the stomach cancer and the like.

The present invention provides a fast method for determining the length of chromosome telomere by flow cytometry, adding an operation of avoiding light and ice bath before the conventional denaturation hybridization step, so that the telomere probe has enough time to enter the inside of the cell, and After the DNA is deformed, it can quickly and effectively hybridize with the telomeric DNA, improving the efficiency and success rate of hybridization; using 1% paraformaldehyde as the fixative, the fixation effect is better, more stable, and more convenient to use; in the hybridization buffer Adding 70% deionized formaldehyde in the probe can protect the stability of cell structure and DNA during long-term probe hybridization, so as to improve the success rate of hybridization. Through the above improvements, the method is faster and more accurate than the traditional method for measuring the length of chromosomal telomeres, and is suitable for long-term and large-scale research work.