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Hydrogen sulfide colorimetric sensor based on G4-Cu<2+> mimic enzyme system

A colorimetric sensor, g4-cu2 technology, applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc.

Active Publication Date: 2019-10-01
SHANGQIU NORMAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to Cu 2 + with S 2- There is a large binding constant between them, and the H 2 There have been many sensors for S detection in recent years, but based on G4-Cu 2+ Mimetic enzyme complex for H 2 The colorimetric sensor for S detection has not been reported

Method used

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  • Hydrogen sulfide colorimetric sensor based on G4-Cu&lt;2+&gt; mimic enzyme system
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  • Hydrogen sulfide colorimetric sensor based on G4-Cu&lt;2+&gt; mimic enzyme system

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Embodiment 1

[0028] (1) Preparation of solution

[0029] Weigh morpholineethanesulfonic acid (MES) and dissolve it in water, prepare 10mM 3,3,5,5-tetramethylbenzidine (TMB) buffer solution, adjust the required pH by pH meter; DNA (sequence 1 ) samples were annealed in 10mM MES buffer (pH7.0) at 95°C for 5min, then cooled slowly to room temperature, and frozen for later use.

[0030] (2) Circular Dichroism Determination

[0031] Five kinds of 10mM MES buffer mixture (pH4.0) containing different substances: (1) Blank; (2) DNA alone, namely 0uMCu 2+ ; (3) 2.2uM Cu 2+ ; (4) 4.4uM Cu 2+ ; (5) 8.8uM Cu 2+ , the circular dichroism spectrum was measured in the range of 220nm to 320nm, the scanning speed of the spectrum was 100nm / min, the response time was 0.5s, and the spectrum obtained by scanning was the average value of three scans. Buffer solution background has been subtracted from CD data.

[0032] (3) Colorimetric detection

[0033] Add CuCl to the above buffer solution 2 and DNA (s...

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Abstract

The invention belongs to the technical field of biological detection sensors, and discloses a hydrogen sulfide colorimetric sensor based on a G4-Cu<2+> mimic enzyme system. In MES (Morpholineethanesulfonic acid) buffer solution, independent Cu<2+> does not have an obvious catalytic function on H2O2 oxidation TMB (tetramethylbenzidine); after human telomere DNA (deoxyribonucleic acid) is added, human telomere can form a G-quadruplex, and meanwhile, the human telomere can be combined with Cu<2+> to form a G4-Cu<2+> mimic enzyme compound, and catalytic capacity is greatly improved; after H2S is met, Cu<2+> and H2S have good specificity, and CuS precipitates are generated so as to lower the enzyme catalytic capacity of G4-Cu<2+>; and along with the increasing of H2S concentration, TMB color development is gradually changed into colourless from blue. By use of the sensor, the color change of the system of the sensor can be used for qualitatively and quantitatively detecting H2S. Due to theadding of the G-quadruplex, the detection sensitivity of a sensing system for H2S is improved, the detection limit of 7.5nM is realized, and the sensing system has good selectivity for H2S.

Description

technical field [0001] The invention belongs to the technical field of biological detection sensors, and relates to a sensor based on G4-Cu 2+ A hydrogen sulfide colorimetric sensor imitating an enzyme system. Background technique [0002] Hydrogen sulfide (H 2 S) is the third gas signal molecule after the discovery of nitric oxide (NO) and carbon monoxide (CO). It is a colorless, poisonous gas with a smell of rotten eggs. H 2 S is produced in the human body through enzymatic and non-enzymatic processes, and can act as a cytoprotective substance and a gas transmitter in many tissues, and can regulate blood vessel tension and cranial nerves. Many studies have found that H 2 Abnormal levels of S may cause some diseases, such as: ischemic stroke, Alzheimer's disease, Parkinson's disease, Down's syndrome, traumatic brain injury and so on. Therefore, real-time monitoring of H in vivo 2 The change of S content is beneficial to the early diagnosis and treatment of related dise...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 耿凤华王永祥马雨徐茂田王丹丹
Owner SHANGQIU NORMAL UNIVERSITY
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