73 results about "Somatic portion" patented technology

There is provided a nuclear reprogramming factor for a somatic cell, which comprises a gene product of each of the following three kinds of genes: an Oct family gene, a Klf family gene, and a Myc family gene, as a means for inducing reprogramming of a differentiated cell to conveniently and highly reproducibly establish an induced pluripotent stem cell having pluripotency and growth ability similar to those of ES cells without using embryo or ES cell.

A method for generating a transgenic soybean plant comprising in its genome a heterologous nucleic acid sequence of interest, comprises introducing into a soybean somatic embryo a polynucleotide encoding a functional dihydrodipicolinate synthase (DHPS) polypeptide, and a polynucleotide encoding a heterologous polypeptide of interest, operably linked to expression control sequences, wherein DHPS expressed from the introduced DHPS-encoding polynucleotide is effective to render the embryo resistant to S-2-aminoethylcysteine (2-AEC), and contacting the embryo with 2-AEC, under conditions effective for an embryo which expresses the DHPS to grow selectably and mature into a soybean plant that expresses a desired trait, and preferably includes no antibiotic resistance marker sequence.

The present invention generally relates to a method for developing, generating and selecting tumor-free embryonic stem (ES)-like pluripotent cells using electroporation delivery of an inducible tumor suppressor mir-302 agent into mammalian cells. More particularly, the present invention relates to a method and composition for generating a Tet-On / Off recombinant transgene capable of expressing a manually re-designed mir-302 microRNA (miRNA) / shRNA agent under the control of doxycyclin (Dox) in human somatic / cancer cells and thus inducing certain specific gene silencing effects on the differentiation-associated genes and oncogenes of the cells, resulting in reprogramming the cells into an ES-like pluripotent state.

The invention discloses a Chinese scholartree detached leaf somatic embryo induction rapid-breeding method. The method comprises selecting Chinese scholartree tender twigs, disinfecting the tender twigs, taking stems with axillary buds or terminal buds, inoculating a bud initial medium with the axillary buds or terminal buds, inducing germination of the axillary buds or terminal buds, removing base leaves of the produced young sprouts, carrying out cutting to obtain stem sections with axillary buds or terminal buds, inoculating a test-tube plantlet multiplication medium with the stem sections with axillary buds or terminal buds to obtain sterile test-tube plantlets, taking leaves of the sterile test-tube plantlets, inoculating a somatic embryo inducing medium with the leaves of the sterile test-tube plantlets, carrying out culture to obtain callus, inoculating an adventitious bud induction medium with the callus, carrying out culture to obtain multiple shoots, cutting the multiple shoots to obtain callus blocks, inoculating a test-tube plantlet multiplication medium or an adventitious bud induction medium with the callus blocks, carrying out culture, transferring the cultured multiple shoots into a shoot strengthening medium, carrying out shoot strengthening culture and carrying out seedling hardening and transplanting. The method has a high regeneration rate, a large budding rate, a plant transplanting survival rate of 95% or more, produces strong seedlings and effectively solves the problem of degeneration of excellent seedling germplasm.

The invention relates to a method for tissue culture and rapid reproduction of wild P.japonicus, comprising (1)selection and sterilization of explants: selecting stems and leaves of wild P.japonicus, surfacely sterilizing same, as explants for tissue culture; (2)callus culture; (3)callus proliferation to form embryogenic callus; (4)somatic embryos differentiation: somatic embryos differentiate from the surface of embryogenic callus; (5)somatic embryo proliferation to differentiate somatic embryos further; (6)plants regeneration culture to form full plants with roots, stems and leaves; (7)strong seedlings culture; (8)plants transplantation. The invention is provided with high frequency of regeneration plants, fast speed of proloferation, and no shortcoming of easy variation of offspring reproduced by normal seeds.

The present invention provides a composition for treating ischemic diseases or neurogenic inflammation. PSA-NCAM-positive neural progenitor cells used in the present invention promote angiogenesis in injected tissue and inhibit an inflammatory response. The PSA-NCAM-positive neural progenitor cells can be simply isolated by using an anti-PSA-NCAM-antibody, and exhibit excellent angiogenic and anti-inflammatory activities compared with mesenchymal stem cells, and thus can be useful as a composition for effectively treating ischemic diseases caused by a vascular injury and nerve damage diseases caused by inflammation. In addition, a secretome of the neural progenitor cells of the present invention reduces the ischemic injury site and allows a neurological function to recover, and thus can be used as an agent for treating ischemic diseases and degenerative nervous system disorders such as nerve damage diseases caused by inflammation.

Cell culture conditions for the isolation, maintenance, and indefinite expansion of human glia are established favoring the growth of neural precursor cells. Cultured cells proliferate indefinitely, express catalytic telomerase, and retain a non-immortalized phenotype. Compositions allow for the indefinite expansion of non-immortalized neural tissue for bioassay applications and restorative neuroscience.

The invention relates to a kit and a method for detecting an anthurium andraeanum SERK gene expression status, and belongs to the field of plant molecular biology. According to the invention, a just-expended young leaf of an anthurium andraeanum potted variety Alabama mature plant is adopted as an explant, and anthurium andraeanum embryogenic callus induction is carried out; non-embryogenic callus and embryogenic callus obtained in two induction stages are subjected to cytological observation; a full-length sequence of cDNA gene of anthurium andraeanum somatic embryo SERK gene is obtained by cloning; primers with relatively high specificity are designed; and researches and analysis upon changes of expression amount of SERK gene transcriptional level of anthurium andraeanum somatic embryo induction and development stages are carried out by using a real-time fluorescent quantitative PCR technology, such that a low-cost and high-specificity kit used for detecting anthurium andraeanum SERK gene expression status is obtained, and a method thereof is provided.