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Chinese scholartree detached leaf somatic embryo induction rapid-breeding method

A technology of isolated leaves and somatic embryos, applied in the field of plant biology, can solve the problems of less budding and low regeneration rate of Sophora japonica, and achieve the effect of saving time.

Active Publication Date: 2016-06-15
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the above problems, and to provide a rapid propagation method for the induction of somatic embryos from isolated leaves of Sophora japonica, so as to solve the problems of low regeneration rate and few buds of Sophora japonica.

Method used

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  • Chinese scholartree detached leaf somatic embryo induction rapid-breeding method
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  • Chinese scholartree detached leaf somatic embryo induction rapid-breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Select a single plant with strong growth and excellent performance, take its young shoots that are free from diseases and insect pests, remove excess stems and leaves, wash carefully with detergent, and rinse with running water for 1 hour. After cleaning, cut into appropriate sizes and prepare for disinfection .

[0049] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 10 minutes, rinse with sterile water for 5 times, and dry the water with sterile filter paper , and then cut about 1 cm of the stem segment with an axillary bud or terminal bud, and insert it vertically into the bud initiation medium (MS+5.0g / L agar+30g / L sucrose, pH 5.8). Three stem segments were inoculated in each bottle to induce the germination of terminal buds and axillary buds. After 15 days of cultivation, the axillary buds or terminal buds of the stems begin to germin...

Embodiment 2

[0061] Select a single plant with strong growth and excellent performance, take its young shoots that are free from diseases and insect pests, remove excess stems and leaves, wash carefully with detergent, and rinse with running water for 1 hour. After cleaning, cut into appropriate sizes and prepare for disinfection .

[0062] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 40 seconds, rinse with sterile water for 4 times, then sterilize with 0.1% mercuric chloride for 12 minutes, rinse with sterile water for 6 times, and dry the water with sterile filter paper , and then cut about 1 cm of the stem segment with an axillary bud or terminal bud, and insert it vertically into the bud initiation medium (MS+5.0g / L agar+30g / L sucrose, pH 5.8). Inoculate 3 stem segments per bottle to induce bud germination. After 18 days of cultivation, the axillary buds or terminal buds of the stems began to germinate, and the stem tips elongated obviously, and t...

Embodiment 3

[0073] Select a single plant with strong growth and excellent performance, take its young shoots that are free from diseases and insect pests, remove excess stems and leaves, wash carefully with detergent, and rinse with running water for 1 hour. After cleaning, cut into appropriate sizes and prepare for disinfection .

[0074] Put the test material on the ultra-clean workbench, disinfect with 70% alcohol for 60 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 15 minutes, rinse with sterile water for 6 times, and dry the water with sterile filter paper , and then cut about 1 cm of the stem segment with an axillary bud or terminal bud, and insert it vertically into the bud initiation medium (MS+5.0g / L agar+30g / L sucrose, pH 5.8). Inoculate 3 stem segments per bottle to induce bud germination. After 20 days of cultivation, the axillary buds or terminal buds of the stems begin to germinate, and the stem tips elongate obviously, and th...

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Abstract

The invention discloses a Chinese scholartree detached leaf somatic embryo induction rapid-breeding method. The method comprises selecting Chinese scholartree tender twigs, disinfecting the tender twigs, taking stems with axillary buds or terminal buds, inoculating a bud initial medium with the axillary buds or terminal buds, inducing germination of the axillary buds or terminal buds, removing base leaves of the produced young sprouts, carrying out cutting to obtain stem sections with axillary buds or terminal buds, inoculating a test-tube plantlet multiplication medium with the stem sections with axillary buds or terminal buds to obtain sterile test-tube plantlets, taking leaves of the sterile test-tube plantlets, inoculating a somatic embryo inducing medium with the leaves of the sterile test-tube plantlets, carrying out culture to obtain callus, inoculating an adventitious bud induction medium with the callus, carrying out culture to obtain multiple shoots, cutting the multiple shoots to obtain callus blocks, inoculating a test-tube plantlet multiplication medium or an adventitious bud induction medium with the callus blocks, carrying out culture, transferring the cultured multiple shoots into a shoot strengthening medium, carrying out shoot strengthening culture and carrying out seedling hardening and transplanting. The method has a high regeneration rate, a large budding rate, a plant transplanting survival rate of 95% or more, produces strong seedlings and effectively solves the problem of degeneration of excellent seedling germplasm.

Description

technical field [0001] The invention relates to a rapid propagation method for induction of somatic embryos from isolated leaves of Sophora japonica, belonging to the field of plant biotechnology. Background technique [0002] National Sophora japonica (Sophora japonicaLinn), also known as Sophora japonica Linn, Chinese Sophora japonica, belongs to Papilionaceae, Sophora genus. Chinese pagoda tree is a deciduous tree with beautiful tree vigor, long flowering period, extremely resistant to pruning, cold resistance, drought resistance, and air pollution resistance. It is also a symbol of auspiciousness, happiness and beauty. It is an ideal tree species for street and garden greening. The material is excellent, the flowers, fruits, root bark, and bark can be used as medicine, and the seeds can be squeezed to make soap. It has high economic value and has a very broad application prospect. At present, the national pagoda tree is the only rootstock tree species for grafting ornam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李双云杨国良李丽庞彩红刘盛芳王守国张炳孝夏阳
Owner SHANDONG FOREST SCI RES INST
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