31 results about "Sequence dependence" patented technology

Method of detecting a target polynucleotide based on the use of dual inversion hybridization probes having stem-and-loop structures, wherein the stem portion of the structure comprises a pair of interactive arms that are substantially prevented from interacting with target polynucleotides. The arms of the dual inversion hybridization probes interact in a conventional antiparallel fashion, but have backbone polarities opposite that of the target-complementary loop portion of the probe. Arm portions of the dual inversion probes do not substantially contribute to sequence-dependent stabilization of probe:target hybrids. Incorporating inversion linkages into the structures of these probes dramatically simplifies the process of designing stem-and-loop hybridization probes.

The present invention relates to methods for generating a labelled nucleic acid from an RNA comprising a 5′ protecting group, said method comprises the steps of obtaining a mixture of template strands of nucleic acids, said mixture comprising said RNA and further potentially other nucleic acids without a 5′ protecting group, annealing at least one oligonucleotide primer to the template strand of said RNA and potentially other nucleic acids, and template sequence dependent extending said primer, thereby obtaining a complementary nucleic acid strand annealed to its template strand, or providing the RNA in duplex with a complementary nucleic acid strand annealed to its template strand, andoptionally modifying the extension product of said nucleic acids without 5′ protecting group either on the 5′ end of the template strand or on the 3′ end of the complementary strand, or both, and labelling a complementary nucleic acid of a double stranded nucleic acid not modified, wherein therefore the labelled nucleic acid did have a 5′ protecting group on the RNA template strand, said 5′ protecting group being optionally removed after modification, and / or labelling a complementary nucleic acid of a double strand dependent on the presence of the 5′ protecting group on the complementary nucleic acids template RNA strand by double strand dependent ligation, thereby specifically generating a labelled nucleic acid complementary to an RNA at least originally comprising a 5′ protecting group.

A signal-off-quantum-dot-based sensor for detecting the presence of a target molecule comprising: an aptamer probe having a nucleotide sequence which specifically interacts with the target molecule by sequence-dependent interaction, wherein the aptamer probe is sandwiched between (a) an oligonucleotide which is immobilized on the surface of a quantum dot (QD), and (b) a fluorophore-labeled oligonucleotide, wherein when the sensor is excited by an energy source: (i) in the absence of specific interaction between the target molecule and the aptamer probe, a baseline signal is emitted, and (ii) in the presence of specific interaction between the target molecule and the aptamer probe, a detection signal is emitted, wherein the baseline signal is greater than the detection signal, whereby the presence of the target molecule is detected.

The invention discloses a method for detecting microRNA by low-sequence-dependent high-order constant temperature exponential amplification. The method designs specific templates T1 and T2 capable of detecting target microRNA, and incises the templates T1 and T2, DNA polymerase, and nick endo The enzyme and dNTP are mixed and reacted at 35-50°C, the amplification curve is detected, and whether the sample contains the target microRNA is determined according to the amplification curve and the standard curve. The method of the present invention can further calculate the specific content of the target microRNA in the sample. The method of the present invention has high amplification efficiency and high sensitivity, and shortens the analysis time; the method has little sequence dependence on the target microRNA and has strong versatility; the detection linear range is wide and the specificity is good; the amplification system is simple and can be developed into a kit and promote it to the market.

Provided are techniques for determining a recovery time for a resource in a heterogeneous computing environment comprising interdependent resources. A graph for the resource representing all sequence dependencies and all group relations are created. The recovery time may be a cumulative startup time or a cumulative shutdown time of the resource considering interdependencies of the resource to other resources. The recovery time for all support resources having sequence dependencies with the resource is calculated and each node representing the support resources are removed from the graph. Then the recovery time for all member resources left in the graph that have group relations with the resource is calculated per a group type of the resource. The recovery time for the resource is a sum of the recovery time of all support resources, the recovery time of all member resources, and a unit recovery time of the resource.

The invention discloses a probe and method for simultaneously detecting multiple microRNAs under constant temperature conditions based on DNA assembly; the probe of the invention includes 3 probe groups; the 3 probe groups and the target microRNA can self-assemble to form a stable " "I"-shaped structure; combined with the high specificity of restriction endonucleases and the cutting properties of single-stranded DNA, DNA modules with sequence-independent nuclease functions are realized, which are applied to the simultaneous fluorescence detection of multiple miRNAs under constant temperature conditions . Solve the complex problem of the conventional constant temperature amplification method system, and provide a new method for the simultaneous and sensitive detection of multiple miRNAs. This method only needs to add restriction endonucleases and probes with simple design, and can achieve constant temperature signal amplification and detection of nucleic acids without adding dNTPs, and has good accuracy, repeatability and stability, and is suitable for development as a kit and promote it to the market.