40 results about "Secondary cell wall" patented technology

The present invention provides methods for modulating cellulose, hemicellulose and lignin composition and deposition in secondary cell wall layers of plants to improve plant traits that are commercially desirable (e.g., enhanced digestibility of forage crops by animals, increased post-harvest processing of wood and crops for energy production and pulping, increase mechanical strength of plants, and others). The invention also provides methods for identifying genes encoding transcription factors that regulate the formation of secondary cell walls, polynucleotide sequences that encode key components of secondary cell walls, and transgenic plants comprising these sequences.

The invention provides methods of engineering plants having lignin deposition or xylan deposition that is substantially localized to the vessels of xylem tissue in the plant. The invention also provides methods of engineering plants to increase production of a desired biosynthetic product, e.g., to have increased secondary cell wall deposition or increased wax / cutin accumulation. The engineered plants of the present invention can be used for bioenergy production, e.g., by improving the density and the digestibility of biomass derived from the plant and to improve water usage requirements.

A method of engineering a plant having reduced acetylation in a cell wall is disclosed. The method comprising expressing in the plant cell wall at least one isolated heterologous polynucleotide encoding an acetylxylan esterase (AXE) enzyme under the transcriptional control of a developmentally regulated promoter specifically active in the plant cell wall upon secondary cell wall deposit, thereby engineering the plant having reduced acetylation in the cell wall.

The invention relates to application of a populus trichocarpa PtrbHLH186 gene in regulation and control of secondary xylem development of trees, and belongs to the technical field of gene engineering. In order to solve the problems that the need for wood quantity and quality is increased day by day and the biological function of the PtrbHLH186 transcription factor is unknown, a PtrbHLH186 overexpressed populus trichocarpa transgenic plant is obtained through an agrobacterium-mediated populus trichocarpa genetic transformation system, and then a series of technical means are utilized for analysis and discovery that the PtrbHLH186 overexpressed populus trichocarpa transgenic plant is found to have the advantages that the PtrbHLH186 overexpressed populus trichocarpa transgenic plant; the overexpression of the PtrbHLH186 transcription factor affects the growth and development of plants and the formation of wood, which is mainly embodied in that the lignin synthase gene is regulated by the gene to affect the deposition of lignin in the secondary cell wall of a tree, so that the morphological structure and distribution of xylem cells are changed; the results show that the PtrbHLH186 transcription factor has a certain regulation effect on the development of the secondary xylem of the tree. The research content provided by the invention has important theoretical guiding significance for cultivating new varieties of high-quality woods and forests.

The invention provides a wood fiber microfilament dissociation method based on a soft nanometer paper base material. The wood fiber microfilament dissociation method comprises the following steps: cutting off and cutting short paper pulp fibers by a pulping machine, and controlling the length of the cut fibers between 500 micrometers and 1000 micrometers; squashing the cut paper pulp fibers by hammering force which is perpendicular to the axial direction of the fibers until the primary cell wall and secondary cell wall outer layers of the fibers are broken or peeled; then utilizing rubbing and shearing force perpendicular to the axial direction of the fibers to separate microfilament layers so as to obtain primarily nano-crystallized fiber microfilaments; finally carrying out high-shear flattening treatment on the primarily nano-crystallized fiber microfilaments to obtain the soft nanometer fibers with uniform size. The method has the advantages of high product yield, uniform size and capability of batch preparing.

The present invention provides polynucleotides and related polypeptides of the class of genes involved in maize secondary wall (ZmSCW) formation. The invention provides genomic sequence for the ZmSCW genes. ZmSCW are responsible for controlling plant growth, secondary cell wall development and yield in crop plants.

The invention relates to a related gene for regulating and controlling wood development and application thereof, and particularly provides a novel PtMAN6 polypeptide or a coding sequence thereof. An amino acid sequence of the PtMAN6 polypeptide is shown as SEQ ID NO.:2, and the coding sequence is shown as SEQ ID NO.:1.The invention further provides a PtMAN6 gene and application of protein coded by the PtMAN6 gene. The PtMAN6 gene can reduce synthesis of xylem secondary cell walls and / or reduce accumulation of crystalline fiber biomass; mannan hydrolase of mannan hemicellulose can be enriched and degraded in plants by excessively expressing the PtMAN6; meanwhile, due to the regulating and controlling effect of the PtMAN6, the lignin content in transgenic plants is decreased, crystalline cellulose is decreased, and therefore the cost in lignocelluloses ethanol producing and paper making industry is greatly reduced; a promoter of the PtMAN6 gene has the specific duct cell positioning function and can precisely regulate and control expression of the gene in xylem duct cells.

Methods and means are provided for the modification of the reactivity of plant secondary cell walls, particularly in cotton cell walls found in cotton fibers. This can be conveniently achieved by expressing a chimeric gene encoding a Saprolegnia monoica chitin synthase in cotton plants.

The invention provides a wood fiber microfilament dissociation method based on a soft nanometer paper base material. The wood fiber microfilament dissociation method comprises the following steps: cutting off and cutting short paper pulp fibers by a pulping machine, and controlling the length of the cut fibers between 500 micrometers and 1000 micrometers; squashing the cut paper pulp fibers by hammering force which is perpendicular to the axial direction of the fibers until the primary cell wall and secondary cell wall outer layers of the fibers are broken or peeled; then utilizing rubbing and shearing force perpendicular to the axial direction of the fibers to separate microfilament layers so as to obtain primarily nano-crystallized fiber microfilaments; finally carrying out high-shear flattening treatment on the primarily nano-crystallized fiber microfilaments to obtain the soft nanometer fibers with uniform size. The method has the advantages of high product yield, uniform size and capability of batch preparing.

A microcosmic identification method for the stem strength of cut peony flowers, comprising the following steps: (1) fixing the stems of cut flowers of peony: cutting the stems of cut flowers of peony and fixing them in pentylene glycol + paraformaldehyde solution; (2) adding (3) Observation of the thickness of the secondary cell wall thickened by the xylem; (4) Statistical analysis of the thickened cell layer and the thickness of the secondary cell wall of the xylem secondary wall of the peony cut flower stalk, through which Two indicators were used to identify the stem strength of peony cut flowers. The method adopted in the present invention can be directly observed and identified according to the microstructure, is convenient and simple, has no interference from human factors, and has low error; and is not limited by time, the sample can be preserved and observed for a long time, and repeated measurements can be made, and the method has high feasibility.

The invention belongs to the fields of molecular biology and genetic engineering, and particularly relates to a Gts (glycosyltransferases) key gene PeIRX10 taking part in xylan synthesis. The gene has the nucleotide sequence shown as SEQ ID No:1. The invention provides a powerful tool for knowing the formation of the secondary cell wall and illuminating the molecular basis of the fast growth of phyllostachys edulis tissues. The effects that the xylan synthesis defects in arabidopsis mutants can be overcome by using the PeIRX10, and the defects of the secondary cell wall in the arabidopsis mutants can be overcome in a complementary way are achieved.

Methods and means are provided for the modification of the reactivity of plant secondary cell walls, particularly in cotton cell walls found in cotton fibers. This can be conveniently achieved by expressing a chimeric gene encoding a Saprolegnia monoica chitin synthase in cotton plants.