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Rice brittle stem regulatory gene DBC2 and application thereof

A technology for regulating genes and rice, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., and can solve the problems that rice brittle stem characteristics cannot be applied and other problems

Active Publication Date: 2020-07-31
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are 8 rice brittle stem mutants reported, all of which have been gene mapped (Singh, K. et al, 1994), and 1 has been proved to be related to the insertion of DS transposable elements (Zhang Fangmei et al, 2002), but it is far from complete There is still a certain distance to uncover the mechanism of rice brittle stalks, and the application of some rice brittle stalk characteristics in production has not yet been applied

Method used

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  • Rice brittle stem regulatory gene DBC2 and application thereof
  • Rice brittle stem regulatory gene DBC2 and application thereof
  • Rice brittle stem regulatory gene DBC2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Obtaining and phenotypic identification of embodiment 1 mutant

[0079] The mutant dbc2 comes from Jinhui No. 10 (Jin10) ethyl methanesulfonate EMS mutagen library. After 8 consecutive generations of selfing, the mutant traits have been stably inherited.

[0080] Under natural cultivation conditions in the field, from the seedling stage, the plant height of dbc2 was significantly reduced compared with the wild type, especially at the mature stage, the plant height of dbc2 was significantly reduced by 26.98% compared with the wild type ( figure 1 A, B, G). Compared with the wild type, the number of internodes of dbc2 had no significant change, and the length of internodes was reduced to varying degrees compared with the wild type ( figure 1 C), the internode lengths from inverted 1 to inverted 6 were significantly reduced by 29.67%, 28.74%, 22.46%, 28.62%, 30.82% and 17.07% respectively ( figure 1 h). At the same time, it was found that compared with the wild type, th...

Embodiment 2

[0082] Example 2 Analysis of yield traits of dbc2

[0083] At the mature stage, the yield traits of the wild type and dbc2 were statistically analyzed, and the results showed that the grain length of dbc2 had no significant change, but the grain width, thickness and thousand-grain weight all increased significantly by 12.76%, 13.82% and 12.26% ( figure 1 F; figure 2 D, A). Husk weight and brown rice weight are two components of grain weight. In order to explore the direct cause of dbc2 grain thousand-grain weight increase, the inventor carried out statistical analysis respectively on the length, width, thickness, thousand-grain weight and glume thousand-grain weight of brown rice. The results showed that compared with the wild type, the length of dbc2 brown rice had no significant change, the thickness increased significantly by 7.90%, and the thousand-grain weight increased significantly by 8.21%, which was consistent with the change trend of the grain; the width was signif...

Embodiment 3

[0084] Example 3 dbc2 tensile measurement analysis

[0085] Under natural growth conditions in the field, the investigation found that the stalks of dbc2 were more likely to be broken than the wild type. The culm is one of the important aboveground organs of rice, in which the sclerenchyma and vascular bundles provide mechanical support for the rice plant. In order to further understand the difference between dbc2 and the wild type in the mechanical strength of the stalk, at the mature stage, the tensile force was measured between the inverted two nodes of the wild type and dbc2, and it was found that the mechanical strength of dbc2 was 38.21% of the wild type, and the degree of decline reached extremely significant level ( image 3 A); The elongation length of dbc2 was 53.71% of the wild type when breaking, and the change of elongation length reached a significant level when breaking ( image 3 B). Therefore, the mutation of DBC2 gene will lead to a significant decrease in...

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Abstract

The invention belongs to the technical field of rice breeding, and particularly relates to a rice brittle stem regulatory gene DBC2 and application thereof. The invention discloses the rice brittle stem regulatory gene DBC2, the nucleotide sequence of the gene is shown as SEQ ID No.1, and a protein encoded by the gene is glucoside hydrolase OsGH9A3 with an amino acid sequence shown as SEQ ID No.2.The rice brittle stem regulatory gene DBC2 disclosed by the invention regulates rice brittle stem traits and dwarfing traits by directly or indirectly influencing the expression quantity of celluloseand lignin synthesis genes and secondary cell wall growth related transcription factor genes, and the regulation of the expression of the brittle stem traits is also regulated by temperature. The rice brittle stem regulatory gene DBC2 is applied to rice improvement, rice with brittle and tender stems is expected to be modified, the brittle and tender stems can be used for feed production, and theproblem that existing rice stems are inconvenient to treat is solved.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, and in particular relates to a rice brittle stem regulation gene DBC2 and its application. Background technique [0002] Rice is the main food crop in China and plays an important role in my country's agricultural production. While rice provides food, it also produces a large amount of straw. Because rice straw has poor taste, is difficult to digest, and is not liked by livestock, it is difficult to use it in feed production. At present, the treatment of rice straw mainly adopts incineration or direct return to the field. The incineration of straw will produce a large amount of smoke and carbon dioxide, which will cause serious pollution to the atmospheric environment. Direct return of straw to the field not only increases the difficulty of farming, but also The probability of occurrence of diseases and insect pests has been increased. Therefore, how to deal with rice straw has become an i...

Claims

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Application Information

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IPC IPC(8): C12N15/56C12N9/24C12N15/82A01H5/00A01H6/46
CPCC12N9/2402C12N15/8261
Inventor 桑贤春王晓雯何光华熊毓贞史岭
Owner SOUTHWEST UNIVERSITY
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