The invention provides a biochemical compound soil heavy metal pollution fixed remediation agent and application, and belongs to the technical field of soil heavy metal pollution control and remediation. The preparing raw materials include sulfydryl bentonite, sulfydryl chitosan, zeolite, iron powder, photosynthetic bacteria, actinomycetes, saccharomycetes and lactic acid bacteria by the weight ratio of 35-65%: 33-52%: 1-8%: 0.2-1%: 0.2-1%: 0.2-1%:0.2-1%:0.2-1%. The fixed remediation agent is effective for unitary or combined heavy metal pollution soil, reduces the effective state and mobility of heavy metal in the soil and therefore ensures safety of soil and plant production, wherein pollution metal includes cadmium, chromium, copper, zinc, mercury, arsenic and lead. The used materials are wide in source, low in cost, remarkable in repair effect and free of secondary pollution, and the fixed remediation agent is environment-friendly soil heavy metal passivation and remediation agent.