The invention discloses a tobacco black shank resistance
single plant identification method. The method includes the steps that tobacco which is about to be inoculated and grows orderly is obtained through tissue culturing rapid propagation, application
inoculation processing is conducted, culture medium slab patches overgrown with black shank hyphae are attached to the rootstocks of
microtrauma tobacco, the treated tobacco is cultivated in a
humidity-controllable
phytotron, resistance distinguish is conducted according to a
research result generated 14 days after
inoculation is conducted, and the resistance is divided according to the morbidity a. The immune or highly-resistant morbidity a is equal to zero, the
disease-resistant morbidity a is larger than zero and smaller than or equal to 25%, the moderately-resistant morbidity a is larger than 25% and smaller than or equal to 45%, the moderately-susceptible morbidity a is larger than 45% and smaller than or equal to 65%, the susceptible morbidity a is larger than 65% and smaller than or equal to 80%, the highly-susceptible morbidity a is larger than 80% and smaller than or equal to 100%, and the immune or highly-resistant,
disease-resistant and moderately-resistant materials are resistance materials. The method is high in identification result reproducibility, good in stability, high in efficiency, capable of achieving large-scale resistance identification and especially suitable for screening
disease-resistant target single plants from a huge number of
mutagenesis populations or
mutant population, hybridization variation populations.