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Method for introducing living cells outside protoplasm microtrauma

A protoplasmic and minimally invasive technology, applied in biochemical equipment and methods, plant cells, botany equipment and methods, etc., can solve the problems of complex operation, inability to process in large quantities, and low efficiency

Inactive Publication Date: 2019-03-15
怀化市共生农业系统工程研究所(普通合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The existing plant hybridization technology has the advantages of high cost, low efficiency, complicated operation, slow effect, and inability to process ma

Method used

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Embodiment Construction

[0014] Below in conjunction with specific embodiment: the present invention is further elaborated and illustrated:

[0015] Obtain exogenous protoplast tissue fluid by pounding juice and pressure filtration of environmental dehydrated biological tissue, or concentrate liquid obtained by low-temperature and low-pressure concentration, or raw juice (unconcentrated) cell slurry to surround and soak biological tissue and seeds, At the same time, by means of concentration, biochemistry, and unfavorable environment, the target material will cause local trauma. The trauma index takes seeds as the material to be introduced, and some seeds will die abnormally. This is the basis for the effect of trauma introduction. The detection of abnormal death is based on the germination rate and the low germination rate under normal conditions. In extreme cases, it may cause 99.9% seed death. When processing seeds in batches, we use about 50% seed death as a reasonable value. In the extreme case, ...

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Abstract

The invention discloses a method for introducing living cells outside protoplasm microtrauma. The method for introducing the living cells outside the protoplasm microtrauma comprises the following steps: using an obtained plant body as a target material; creating a trauma on the target material; obtaining an exogenous protoplasm tissue fluid; and then soaking the target material in the exogenous protoplasm tissue fluid; taking the soaked survival target material and planting the soaked survival target material; and screening a material in which exogenous protoplasm and the target material aresuccessfully hybridized as the target material. The method for introducing the living cells outside the protoplasm microtrauma, disclosed by the invention, has the advantages that a huge genetic relationship distance can be crossed by introducing the exogenous protoplasm, and a purpose of distant asexual crossbreeding is realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for externally introducing living cells through protoplasmic micro-trauma. Background technique [0002] The existing plant hybridization technology has high cost, low efficiency, complicated operation, slow effect, inability to process materials in large quantities, is limited by the specificity of various introduction channels, and has phenomena such as incompatibility of distant hybridization. Contents of the invention [0003] In order to overcome the above problems, the present invention provides a method for externally introducing living cells into living cells through micro-injury of protoplasm. In the invention, the introduction of exogenous protoplasm can span a huge relationship distance and realize the purpose of distant asexual hybrid breeding. [0004] The technical scheme that the present invention solves its technical problem adopts is: [0005...

Claims

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Application Information

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IPC IPC(8): C12N15/05
CPCC12N5/04
Inventor 胡爱生胡心宇
Owner 怀化市共生农业系统工程研究所(普通合伙)
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