The invention belongs to the field of medical
biotechnology, and relates to two series major vault
protein of
glucagon-like
peptide-2 (GLP-2) of people and a preparation method of the two series major vault
protein. A second place of an
amino acid fragment of the
glucagon-like
peptide-2 of people is mutated, two segments are connected in series through connecting
peptide, a
dimmer structure of the GLP-2 is simulated, encoding
gene of the two series major vault
protein of the GLP-2 is obtained by a synthetic method, the encoding
gene is cloned to a pET22b(+)
pronucleus so as to express a vector,
Escherichia coli BL21 is transformed, the encoding
gene is expressed in the
Escherichia coli efficiently, the purified reconstructed purpose protein is obtained through salt
fractionation of
ammonium sulfate in fractional and
chromatography of anion exchange columns. The reconstructed purpose protein can stimulate a Caco-2
cell, multiplication of the Caco-2
cell can be promoted notably, the two series major vault protein of GLP-2 of people has natural biological activities, and therefore a foundation is established for further study and extensive use of the GLP-2 of people.