69 results about "Drosophila buzzatii" patented technology

The invention discloses a botanical insecticide and a preparation method thereof. The effective components of the botanical insecticide comprise the following raw materials in parts by weight: 30-70 parts of camellia seed cakes, 10-30 parts of melia azedarach, 10-30 parts of fructus cnidii, 20-40 parts of radix stemonae and 20-40 parts of chilies. The botanical insecticide disclosed by the invention has a significant effect on controlling varied pests such as aphides, red spiders, core-eating insects and fruit flies on a fruit tree. The botanical insecticide disclosed by the invention has the advantages that the control effect is good, pests cannot have the resistance to the drugs easily, the cost is low, the insecticide is environment-friendly, the preparation method is simple, and the market prospect is great; and the botanical insecticide deserves wide popularization and application in agricultural production.

The invention relates to a slow release fruit fly attractant and application thereof, belonging to the field of pest control. The slow release fruit fly attractant comprises the following main components in percentage by weight: 1-20% of carbohydrate, 5-40% of protein source materials, 0.5-20% of plant allelochemicals, 0.01-10% of toxic components, 0.5-5% of emulsifying agent, 1-15% of moisture regulator and 0.1-5% of preservative. By adding water to the components to 100% and fully and uniformly stirring, a semisolid ointment slow release attractant is obtained to be applicable to the prevention and treatment of fruit flies. The slow release attractant of the invention exists in a semisolid ointment state, can be directly scrawled in a fruit fly attracting bottle, can be mixed with waterfor local spraying, and has the characteristics of very convenient operation, male-female attraction, favorable attraction effect, long duration and safety for the crop.

The invention relates to a method and a device for propagating fruit flies by utilizing a liquid culture medium. The method comprises the following steps that (1) the liquid culture medium is prepared; (2) a fruit fly cage mode is used for feeding adult fries; (3) larvae are fed, and flyblows are obtained: (3-1) a feeding tank for feeding fruit fly larvae is manufactured; (3-2) the liquid culture medium is added into the feeding tank; (3-3) the feeding tank is put into a fly cage; (3-4) fruit flyblows are obtained; (4) after the fruit fly larvae are fed to the aging state, old and mature larvae are eluted and filtered; (5) the larvae are placed in a bag, after the fruit fly larvae are pupated, fruit fly pupae are cut off together with the bag, and undamaged fruit fly pupae are obtained. The method and the device have the advantages that various insect states, particularly older larvae and fruit fly larvae, can be easily obtained, the surfaces of the older larvae and the pupae of the obtained fruit flies are clean, and the pupa body is complete and is not damaged.

A system and method of release and dispersion of genetically altered flies or other insects to control insect population. Disclosed is an apparatus designed to be carried by an aircraft which implements a method of insect population control. The apparatus features one or more removable fly chambers that contain fly containment cylinders. The fly chambers are seated upon one or more nesting chutes. A refrigeration system operates to physically retard the fruit flies. Once the flies have been successfully immobilized by the refrigerated air, they are released from the fly containment cylinders and fall to the bottom of the fly chamber. Gates on the bottom of the fly chamber are opened manually or remotely to allow the refrigerated flies to fall through the nesting chute into the spiral release controllers. The spiral release controllers dispense the genetically altered flies into the exit chute. The genetically altered flies travel through the exit chute, fall to the earth below and mate with the population thus serving to decrease population by capitalizing on a weakness in the reproductive cycle of the fruit fly or other insect.

A male annihilation insecticide formulation, having a viscosity appropriate for use in aerial or backpack spray applications and suitable for on-crop or off-crop uses, comprises an insect toxicant and a male-specific attractant, such as a parapheromone, for a target insect species, such as fruit fly, dispersed and entrapped in an emulsion comprising a biodegradable wax, an emulsifiers, and water.

The invention provides a compound for trapping and killing drosophila melanogaster. The compound is characterized by being prepared from the following raw materials according to ratio: 40-60wt% of Chinese herbal medicine asarum powder, 1-3wt% of brown sugar, 5-10wt% of millet wine, 5-10wt% of rice vinegar and 20-40wt% of water. The compound does not pollute the environment, has no toxic and side effect to human, livestock and fertilization insects, the pesticide effect is long and can be kept for about three months, the cost is low and the compound is cheap.

A wheat bran medium for culturing fruit bat fly in laboratory comprises 10g of wheat bran, 10 g of brown sugar, 1.2 g of agar, 1.2g of yeast and 0.15g of benzoic acid. A preparation method of the medium comprises steps of: dissolving the components in 100 ml of tap water and carrying out an autoclaving; taking glass test tube (25*200 mm) treated with dry heat sterilization (120 DEG C for 2h); subpackaging 20ml of medium into each test tube; cooling to a room temperature until the medium being solid gel; and putting fruit bat fly in the medium, wherein a test tube plug is absorbent cotton treated with dry heat sterilization. The easily prepared medium has low costs, good production effect and strong mildewing resistance, and is convenient for genetics and development biology researches on fruit bat fly.

The invention discloses a method for cultivating organic blueberries in low-latitude and high-altitude areas. The method comprises the steps as follows: selecting fields with the slope gradient not higher than 10 degrees, the soil PH value being 4.0-6.0 and the organic matter content being not lower than 5%, and carrying out field soil preparation with the deep tillage depth being 20-25 cm in the year before field planting; selecting blueberry seedlings to perform field planting in winter or in rainy seasons of the next year, then covering a moist soil surface with a mulching material and performing filed planting on seedlings which are sufficiently watered; performing fruit tree pruning twice including pruning after fruit picking, and pruning and disbranching in winter; adopting drop irrigation or irrigation to maintain the maximum water-holding capacity of the soil to be 60-70%, and applying organic fertilizer in twice every year, wherein applying organic fertilizer after blueberry harvest and before spring-growth on the moist soil surface, and applying fertilizer heavily while ditching to realize water and fertilizer management; trapping the adults and larva of scarabs by adopting light and / or trapping and killing by using willow leaves impregnated with pesticide and performing deep ploughing to enable the larva of scarabs to be exposed to the sun; trapping and killing drosophila melanogasters with sweet lure or yellow sticky boards. The method for cultivating organic blueberries in low-latitude and high-altitude areas has the characteristics that the management is simple and convenient, damage caused by diseases is less, good harvest and high quality of the blueberries are achieved, and the blueberries obtained by adopting the method are purely natural and pollution-free.

The invention relates to an insecticide product killing injurious insects, in particular to fruit fly attractive toxicant and a preparing technology thereof. The fruit fly attractive toxicant comprises fruit and vegetable food, yeast, vinegar, sweetening agents, insecticide and water or comprises a drink, vinegar, sweetening agents and insecticide. The whole attractive toxicant preparing process is nonhazardous and wholly environmentally friendly, cost is low, almost all families can prepare the attractive toxicant, and the attractive toxicant has a very good effect for trapping and killing fruit flies.

The invention discloses a power transmission line icing thickness prediction method based on CEEMDAN-QFOA-LSTM, and relates to the field of combination of power transmission line state evaluation anddeep learning. The method comprises the following steps o: (1) carrying out data acquisition and preprocessing; (2) carrying out CEEMDAN decomposition on an icing thickness historical data sequence (12); (3) optimizing hyper-parameters of the LSTM by a quantum drosophila melanogaster algorithm; (4) carrying out LSTM model training (14); and (5) predicting the icing thickness of a power transmission line and analyzing a result (15). According to the method, the CEEMDAN decomposition algorithm is used, a sequence which is difficult to directly predict is converted into a plurality of predictablecomponent sequences; a neural network can more accurately grasp the law of the sequence according to multi-dimensional feature information obtained through decomposition; a QFOA optimization algorithm is used for obtaining the hyper-parameters, a complex manual parameter adjustment process is avoided, and a network model is trained more effectively; the used LSTM neural network does not have theproblem of gradient disappearance of a general network, so that optimal convergence of the model is ensured, and the problem of short-term and long-term time sequence prediction is effectively solved.

The invention discloses an arbutus tree pruning method facilitating insect net suspension. According to the invention, robust supporting branches are directly cultivated on the periphery of an arbutus tree; inner large branches are thinned; and an entire crown is subjected to dwarfing retraction cutting. Therefore, a natural support formed by arbutus tree large branches is used as a support frame for suspending the insect screen. The insect screen can be suspended on the arbutus plant, such that damages caused by fruit tree pests such as fruit flies can be prevented. The implementation method of the invention is simple, and cost is low. With the method, insect net suspension time is short, and efficiency is high. The harm of pests to arbutus fresh fruits can be substantially reduced, and fruit farmer funding can be substantially reduced. The method has significant economic benefit, and is suitable for popularization.

The present invention relates to a fruit fly attractant, which comprises the following components and their parts by weight: 60-120 parts by weight of fruit, 20-40 parts by weight of yeast, 2-50 parts by weight of sweetener, and 10-40 parts by weight of insecticide parts by weight, 5-15 parts by weight of water, 60-150 parts by weight of flour, and 5-10 parts by weight of preservatives; the fruit fly attractant prepared by the present invention is in powder form, which is convenient for transportation and use, and can be carried out in bags. It is sold, which reduces the cost of making trap bottles, and can be mixed with water or vinegar when used, of course, it can also be used alone, and the effect of attracting fruit flies is good.

The invention provides a method for mass-producing parasitic wasps of fruit flies by means of drosophila melanogaster. By fast and simply collecting pupae of the drosophila melanogaster, mass breedingand parasitism of the parasitic wasps of the fruit flies are simply completed, in a pupal stage, the parasitic wasps of the fruit flies are fast and simply separated from the unparasitized drosophilamelanogaster, and the number and a sex ratio of the parasitic wasps of the fruit flies are fast and simply regulated and controlled, so that full mating and a proper parasitism rate are ensured.

The invention discloses a fruit fly catching device with an attractant having a rapid attracting function, pertaining to the field of pest control. The fruit fly catching device comprises a cup body used for containing attractant having the rapid attracting function, a cup lid, a sun protection cover, a connecting structure and a catching container arranged between the cup body and the cup lid and used for trapping. The cup body comprises a main cup body and an end opening at the upper portion of the main cup body. A first short column and a second short column, used for fixing the catching container and the cup lid, are symmetrically arranged along the end opening. The cup lid comprises a channel arranged on the cup lid and used for ingress of fruit flies, a first fixing part arranged at the center of the upper portion, a first clamping structure and a second clamping structure. The first clamping structure and the second clamping structure are arranged on the periphery of the cup lid and used for clamping the first short column and the second short column. The attractant comprises: (1) fruit and vegetable food, drinks, yeast, edible vinegar, sweeteners, insecticides and water; or (2) drinks, edible vinegar, sweeteners and insecticides. The fruit fly catching device with the attractant having the rapid attracting function is easily assembled and is capable of rapidly trapping fruit flies and is low in manufacturing cost.

The invention relates to a convenient-to-operate feeding bottle for fruit-fly-pupa parasitic wasps. The convenient-to-operate feeding bottle used for facilitating the breeding of the fruit-fly-pupa parasitic wasps comprises three parallel single bottles, guide pipes, opening and closing units and wasp blocking unit, wherein the guide pipes are arranged at the left end and the right end of each single bottle, and every two adjacent single bottles are connected through the guide pipe; the opening and closing units are arranged on the guide pipes and used for opening and closing the guide pipes;the wasp blocking units are arranged at the openings of the single bottles and used for preventing the parasitic wasps from flying out of the single bottles and do not affect the air exchanging insideand outside the single bottles. The convenient-to-operate feeding bottle and the operation method thereof have the advantages that parasitic wasp breeding can be achieved, the parasitic wasps can betransferred among the single bottles without opening the openings of the single bottles, and operation convenience and simpleness are achieved.

The invention provides a DV-hop visible light indoor positioning method based on fruit fly correction. The method comprises the steps that firstly, a mathematical model of visible light indoor positioning is established, and unknown nodes of a visible light system are positioned based on a DV-hop protocol; the optimal anchor node in the DV-hop visible light positioning method is selected by introducing a drosophila melanogaster optimization search algorithm, so that the average distance per hop is closer to the real distance between the anchor node and an unknown node, and the effect of improving the positioning precision is achieved.

The embodiment of the invention discloses a method and a system for classifying data based on an improved fruit fly optimization algorithm. The method comprises the following steps: (1) preprocessingacquired historical data; (2) taking the preprocessed historical data as a training sample, and optimizing parameters (penalty coefficient and kernel width) of a prediction model (support vector machine) by using a new improved fruit fly optimization algorithm; (3) constructing a prediction model according to parameters obtained by algorithm optimization; and (4) classifying the to-be-tested databy adopting the newly constructed prediction model. By implementing the method, the problems of falling into a local optimal solution, insufficient convergence precision and the like of a drosophila melanogaster optimization algorithm can be solved, the problems in the specific field are classified and predicted, and the decision precision of a prediction model is improved.

The invention discloses the use of zanthoxylum leaf total flavonoids extract, which can improve the learning and memory of transgenic Alzheimer disease model of drosophila melanogaster, and has good effect in medicines for treating Alzheimer's disease.

The invention provides an application of 1, 2, 4-trimethoxybenzene or alpha-terpilenol as shown in a formula 1 or a formula 2 in the specification in a learning and memory ability medicine for treating the Alzheimer's disease, pavlovian olfactory memory detection carried out by an A beta transgenic fruit fly model prepared by an elav-Gal4 promoter shows that after 1, 2, 4-trimethoxybenzene or alpha-terpilenol is given to AD model fruit flies, the electric shock avoiding capability of the AD model fruit flies according to odor information is also remarkably improved, the 1, 2, 4-trimethoxybenzene or alpha-terpilenol can improve the learning and cognition ability of AD model fruit flies, and it is revealed that 1, 2, 4-trimethoxybenzene or alpha-terpilenol can be used for preparing the medicine for treating AD.

The invention discloses a fruit fly trapping and killing device. The fruit fly trapping and killing device comprises a plurality of trapping and killing bottles and a supporting frame, and the multiple trapping bottles are hung at the top end of the supporting frame; each trapping and killing bottle comprises a bottom support, a lower bottle body, an upper bottle body and a bottle cap which are sequentially arranged from bottom to top; first necking cylinders extending upwards into inner cavities of the lower bottle bodies are arranged in the centers of the bottoms of the bottom supports; a flaring part is arranged at the top end of each first necking cylinder; a plurality of second necking cylinders is arranged at the top end of each flaring part; a supporting table is arranged in each upper bottle body; a trapping and killing bowl is arranged on each supporting table; and trapping and killing agents are arranged in the trapping and killing bowls. The first necking cylinders extendingupwards into the inner cavities of the lower bottle bodies is arranged in the centers of the bottoms of the bottom supports, the trapping and killing agents attract fruit flies to enter the lower bottle bodies along the first necking cylinders, trapping of the fruit flies is achieved, the lower bottle bodies, the upper bottle bodies and the bottle caps are sequentially fixed in a threaded mode and can be separated in pairs, discharging of the fruit flies and replacement of the trapping and killing agents are facilitated, the cost is low, environmental friendliness is achieved, and the trapping and killing effect is good.

The invention discloses an automatic-liquid-supplementing fruit fly trap which comprises a trapping agent container comprising a main container and a supplemented liquid container. A window for fruitflies to fly in is arranged on the upper portion of the main container, an automatic liquid supplementing device is arranged at the bottom of the main container and comprises a shell fixed at the bottom of the main container, the shell comprises an upper cavity and a lower cavity, a liquid incoming joint is arranged on the side face of the lower cavity, the upper cavity and the lower cavity are communicated through a vertical tube hole, a liquid discharging hole is formed in the upper cavity, a buoyancy block capable of moving up and down is arranged in the upper cavity, a pull rod matched with the vertical tube hole is connected on the buoyancy block, and an end cap is arranged at the lower end of the pull rod; a liquid discharging tube connected with the liquid incoming joint is arrangedon the lower portion of the supplemented liquid container. Through a combined structure of the main container, the supplemented liquid container and the automatic liquid supplementing device, a function of the fruit fly trap in automatically supplementing a trapping agent is realized, and the technical problem that existing fruit fly traps need manual supplementing of the trapping agent is solved.

The invention discloses a biological insecticide screening mode, which comprises the following steps: integrating acceptor EcR with ecdysone GAP with gene expressing box Promoter-EcR-AOX1 TT and super spiral protein USP gene with gene expressing box TEF1 Promoter-USP-CYC1 TT on the colorant layer of Pasteur pichia; integrating DNA fragment with fruit fly 5XEcRE-HSP27 Promoter-GFP on the colorant layer yeast; getting the product. To co-culture screening drug with this product, we can get stopping or reinforcing GFP expression biological insecticide.

The invention discloses a measuring device and method for frog predation and avoidance behavior energy distribution under natural enemy pressures. The device comprises a frog activity area; a naturalenemy activity area is placed at one corner of the frog activity area; the natural enemy activity area comprises a natural enemy breeding box; the natural enemy breeding box is placed at the corner ofthe frog activity area; a natural enemy sound player is mounted on the side wall of the natural enemy breeding box; a replaceable partition plate is installed on the side wall, facing the center in the frog activity area, of the natural enemy breeding box; a fruit fly breeding box is mounted below the frog activity area; fruit flies are conveyed to the frog activity area and the position above the natural enemy breeding box through a first fruit fly channel and a second fruit fly channel from the side wall of the fruit fly breeding box; and a crawling ladder for frogs to climb to the positionabove the natural enemy breeding box is mounted in the frog activity area. According to the measuring device, by replacing different partition plates and additionally arranging the natural enemy sound player, measuring of frog predation and avoidance behavior energy distribution under one or more combined natural enemy pressures is achieved, the structure is simple, operation is convenient, and the testing accuracy is high.

The invention discloses a method for detecting the feed intake of fruit flies, which comprises the following steps: (1) cultivating the fruit flies to be tested using a first solid medium; (2) after the fruit flies complete the first cycle of cultivation , using the second solid medium to continue the second cycle of cultivation; (3) after completing the second cycle of cultivation, the fruit fly excrement is measured; wherein, the first solid medium contains brilliant blue dye, and the second solid medium Contains no dyes. The invention can accurately and continuously measure the feed intake of the fruit flies at multiple time points for a long time without harming the fruit flies.

The patent of this invention relates to the field of biological control of pests, and provides a simple and rapid breeding method for the Drosophila melanogaster pupa parasitoid Wasp trichocystis. The main content of the method is: preparation of fruit fly insect culture tanks and parasitoid wasp insect culture tanks; breeding of spotted wing Drosophila and Drosophila melanogaster; general breeding of Trichoderma striata; preparation of Drosophila melanogaster pupae and parasitoid wasps Parasitism; application of parasitic wasps reared in large numbers. This method is time-saving, labor-saving, has a simple device, and can breed a large number of D. trichophyton in a short time, so as to meet the requirements for large-scale population experiments of this parasitic wasp in the laboratory or to be used for fruit fly control in the field.

The invention relates to the field of autonomous navigation of unmanned boats, and in particular, to a layered obstacle avoidance method for unmanned boats based on improved fruit fly optimization and dynamic window methods. The invention includes: step S1: establishing a geographical environment model for maritime navigation of unmanned boats based on electronic charts; step S2: using an improved fruit fly optimization algorithm to complete global optimal path planning; step S3: establishing an environmental model of dynamic obstacles; Step S4: Use the improved dynamic window method to avoid moving obstacle ships. The present invention provides an obstacle avoidance method that switches between dynamic and static obstacle avoidance modes based on judgment conditions, which can effectively avoid obstacles during navigation, avoid the algorithm from falling into the local optimal solution, and adopt fuzzy control methods to determine the optimal trajectory. The weight parameters are dynamically controlled to improve the accuracy and efficiency of trajectory prediction.

The invention relates to the technical field of biological mediums, in particular to a medium for prolonging the life of fruit flies and preparation and use methods thereof. The preparation method includes the following steps: (1) obtaining a Phellinus igniarius fruit body; (2) preparing a Phellinus igniarius water extract; (3) conducting freeze drying; (4) formulating a mother liquor; (5) conducting diluting; and (6) formulating a medium. The medium containing the Phellinus igniarius water extract is added, and by observing the life of fruit flies, the medium can significantly prolong the life of fruit flies. Compared with a control group, under same feeding conditions including temperature, humidity and light conditions, through calculation on the semi-lethal dose, the added Phellinus igniarius water extract can significantly increase the semi-lethal days by 7d compared with a control medium, thereby significantly prolonging the lifespan of fruit flies.

The invention belongs to the field of biopesticides, and particularly relates to bacillus thuringiensis JXBT-0296 with insecticidal activity on ganoderma lucidum geometridae and application of the bacillus thuringiensis JXBT-0296. The separated and screened bacillus thuringiensis JXBT-0296 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO:M2020071, and the strain is separated from a soil sample collected from Lushan jungle in Jiangxi Province. Bioassay shows that a fermentation product of the strain has strong insecticidal activity on ganoderma lucidum geometridae, and also has repellent activity on ganoderma lucidum drosophila melanogaster; the strain also has an insecticidal effect on ganoderma lucidum noctuid, hapsitera barbata christoph,chilo suppressalis, plutella xylostella or cabbage caterpillar, and is particularly suitable for being developed into a biocontrol fungicide for preventing and treating ganoderma lucidum pests.

The invention relates to the technical field of fruit fly models, in particular to a method for screening infertility related genes by utilizing a fruit fly model and application of the method. The method disclosed by the invention comprises the following steps: S1, mating UAS-X-RNAi transgenic line fruit flies with tub-GAL4 / CyO fruit flies, selecting F1-generation newly-emerged UAS-X-RNAi / tub-GAL4 male / female fruit flies as an experimental group, and selecting UAS-X-RNAi male / female fruit flies as a control group; s2, the F1 generation of newly emerged UAS-X-RNAi / tub-GAL4 male / female drosophila melanogaster and the UAS-X-RNAi male / female drosophila melanogaster obtained in the step S1 are mated with wild type female virgin drosophila melanogaster / male drosophila melanogaster; s3, observing and counting the number and hatching rate of offspring eggs and adults of the experimental group and the control group in the step S2; the X gene is a gene to be screened.