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A simple and rapid propagation method of Drosophila spotted wing pupae parasitic hornet horn beetle

A technology of Drosophila spotted wing and parasitic wasp, which is applied in the field of simple and rapid indoor propagation of the parasitic wasp Ceratia cerevisiae in the pupal stage of Drosophila spotted wing, can solve the problem of being unable to meet the needs of large-scale reproduction, the process is cumbersome and complicated, and the black-bellied Problems such as fruit fly contamination

Inactive Publication Date: 2021-09-07
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0004] The Chinese patent with the application number CN201610213523.3 discloses a method for indoor breeding of the horny hornet, which is roughly the same as the general breeding method of the parasitic wasp in step (3) of this method, and is only suitable for keeping the parasitic samara in the laboratory Stable population of fly parasitoids; because the process of breeding Drosophila melanogaster is cumbersome and complicated, it is easy to be polluted by Drosophila melanogaster, and the number of artificially reproduced offspring is small, so the use of Drosophila melanogaster as a host cannot meet the needs of a large number of parasitoids
The Chinese patent application number CN201710749534.8 discloses a production method for the artificial breeding of pupal parasitoids that strengthens the biological control effect of fruit flies. , life expectancy and other aspects have been enhanced, but in the process of operation, manpower is needed to pick fruit fly pupae, which naturally cannot meet the needs of mass reproduction

Method used

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  • A simple and rapid propagation method of Drosophila spotted wing pupae parasitic hornet horn beetle
  • A simple and rapid propagation method of Drosophila spotted wing pupae parasitic hornet horn beetle
  • A simple and rapid propagation method of Drosophila spotted wing pupae parasitic hornet horn beetle

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Embodiment 1

[0033] Acquisition of the species of the horned horned horned wasp:

[0034] The parasitic wasp utilized in the method—Herpes pilaris is from trapping in the field. Design of trapping device: This device is refitted from a 500ml mineral water bottle, and the body of the bottle is drilled with a small hole with a diameter of 0.5cm. For the convenience of hanging the trapping bottle on the fruit tree, the cap is drilled and tied with an iron wire. Drosophila feed containing Drosophila spotted wing eggs, larvae and pupae in each developmental stage. During the period when the berry fruit flies harm the orchard, hang the trapping device under the bayberry tree (about 1m above the ground on the shady side), observe the trapping situation every day, and bring the trapped adult parasitoid bees and the host in the device back to the insect culture room breed.

[0035] After trapping, the hosts in different periods in the device were transferred to the insect culture room with temper...

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Abstract

The patent of this invention relates to the field of biological control of pests, and provides a simple and rapid breeding method for the Drosophila melanogaster pupa parasitoid Wasp trichocystis. The main content of the method is: preparation of fruit fly insect culture tanks and parasitoid wasp insect culture tanks; breeding of spotted wing Drosophila and Drosophila melanogaster; general breeding of Trichoderma striata; preparation of Drosophila melanogaster pupae and parasitoid wasps Parasitism; application of parasitic wasps reared in large numbers. This method is time-saving, labor-saving, has a simple device, and can breed a large number of D. trichophyton in a short time, so as to meet the requirements for large-scale population experiments of this parasitic wasp in the laboratory or to be used for fruit fly control in the field.

Description

technical field [0001] The invention belongs to the field of biological control of pests, and in particular relates to a simple and rapid indoor breeding method for the parasitic wasp Ceratia chinensis in the pupal stage of Drosophila spotted wing. Background technique [0002] Spotted Drosophila[ Drosophila suzukii (Matsumura, 1931)] also known as Cherry Drosophila, Drosophila Suzuki, the English name is Spotted wing drosophila (SWD), belongs to Diptera (Diptera) ring split suborder (Cyclorrhapha) Drosophilidae (Drosophilidae) Drosophila genus (Drosophila) fruit fly subgenus (Sophophora) Drosophila melanogaster species group. Unlike most fruit flies that feed on rotting fruit, spotted wing Drosophila is different because the female has a hard, serrated ovipositor that can lay eggs in ripe or soon-to-ripe soft-skinned fruits. At present, it is known that the spotted-winged Drosophila can harm more than 60 kinds of fruits in 18 families, including blueberries, raspberries, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01G13/00
Inventor 仪传冬林嘉刘旭祥宋学森季清娥陈家骅
Owner FUJIAN AGRI & FORESTRY UNIV
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