The invention relates to a multi-layer stacking and transferring method for graphene. The method includes the operation steps that S01, the bottom face and the surface of a transferring target substrate are coated with an elastic transparent coating; S02, thermo-compression bonding is conducted on copper foil where graphene grows and the coated elastic transparent coating in a rolling mode; S03, the obtained compressed structure continues to be soaked in an electrolyte solution through rolling pressing, and electricity is applied for bubbling at the same time so that graphene and copper coil can be separated, attached and transferred to the target substrate; S04, washing and blow-drying are conducted on the surface of transferred graphene; S05, the step S02, the step S03 and the step S04 are repeatedly executed, graphene is stacked and transferred on the surface of transferred graphene till graphene is stacked to the required level number. Rapid and complete transferring of graphene is achieved by coating the graphene transferring target substrate with the elastic coating for modification and through the electrochemistry bubbling stripping method, multiple layers of stacked graphene are transferred according to practical requirements, and the electrical property uniformity and stability of graphene are improved.