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30 results about "Cell adhesion factors" patented technology
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Cell Adhesion Factors are small targets expressed by blood vessels and organs to attract immune cells (and 'adhere' them to the cell) and draw them into tissue. Suppressing these factors is immunosuppressive, but may alleviate artherosclerosis.
Nanobioelectronics
The present invention generally relates to nanobioelectronics and, in some cases, to circuits comprising nanoelectronic elements, such as nanotubes and / or nanowires, and biological components, such as neurons. In one aspect, cells, such as neurons, are positioned in electrical communication with one or more nanoscale wires. The nanoscale wires may be used to stimulate the cells, and / or determine an electrical condition of the cells. More than one nanoscale wire may be positioned in electrical communication with the cell, for example, in distinct regions of the cell. However, the nanoscale wires may be positioned such that they are relatively close together, for example, spaced apart by no more than about 200 nm. The nanoscale wires may be disposed on a substrate, for example, between electrodes, and the cells may be adhered to the substrate, for example, using cell adhesion factors such as polylysine. Also provided in other aspects of the invention are methods for making and using such devices, kits for using the same, and the like.
Owner:PRESIDENT & FELLOWS OF HARVARD COLLEGE
Artificial vascular grafts, their construction and use
The present relates to artificial vascular grafts, methods for manufacturing and uses for them. The grafts comprise an inner surface on which cells genetically altered to express or over-express one or more cell adhesion factors or one or more cell adhesion factors and one or more cell proliferation growth factors are seeded and cultured.
Owner:MULTI GENE VASCULAR SYST
Transglutaminase Crosslinked Collagen Biomaterial for Medical Implant Materials
InactiveUS20080305517A1Enhanced ability to support cell attachment cellEnhanced ability to support attachmentBone implantTissue cultureCross-linkMicroorganism
The present invention provides a method for producing an improved biomaterial comprising treating a collagen biomaterial with a transglutaminase under conditions which permit the formation of cross-links within the collagen. Preferably, the transglutaminase is a tissue transglutaminase, a plasma transglutaminase or a microbial transglutaminase. In a preferred embodiment, the collagen biomaterial further comprises a cell adhesion factor, such as fibronectin. The invention further provides biomaterials obtainable by the methods of the invention, and medical implants and wound dressings comprising the same.
Owner:ASTON UNIV
Cell culture support and production method and uses thereof
InactiveUS20100216242A1Increase freedomAnimal cellsCell culture supports/coatingSurface layerAdhesion process
The present teachings provide a practical cell culture support by which a cell culture with a high degree of freedom can be realized. More specifically, the cell culture support includes a polymer layer exhibiting thermoresponsiveness and a cell culture region obtained by plasma-treating a surface layer portion thereof with a reactive gas, whereby a cell culture support having thermoresponsiveness and cellular adhesiveness while avoiding or limiting the use of cell adhesion factors is provided.
Owner:TOYOTA CENT RES & DEV LAB INC
Brain-protective agent
A brain-protective agent containing an NF-κB decoy. In brain diseases, the brain can be particularly effectively protected against brain disorders (for example, cerebral vasospasm following a subrachnoidal hemorrhage and apoptosis of the nerve cells following a cerebrovasucular accident or serious head injury) caused by the undesired activation of cytokines or cell adhesion factors which are regulated by NF-κB by administering the brain-protective agent containing an NF-κB decoy, i.e., acompound antadonistic specifically to a nucleic acid to which NF-κB binds.
Owner:ANGES MG INC
Method for detaching cultured cells, cell detachment device used in said method for detaching cultured cells, and incubator
InactiveUS20120329123A1Bioreactor/fermenter combinationsBiological substance pretreatmentsShock wavePhotothermal conversion
Disclosed is a method for detaching cultured cells that can selectively detach cultured adhered cells. The scaffold to which the cells adhere is configured from at least a cell adhesion factor containing carbon nanotubes, and by means of radiating laser light in a spot shape on the scaffold in a region where cells are adhered, a shock wave is generated by the heat arising by means of the photothermal conversion of the carbon nanotubes, and by means of this shock wave, the cells are caused to be in a non-adhered state.
Owner:JAPAN SCI & TECH CORP
Method for promoting hair growth or hair regeneration by maintaining or increasing expression of cell-adhesion factor
InactiveUS20110112019A1Hair growth hair be promotedPromote regenerationOrganic active ingredientsPeptide/protein ingredientsAdhesion processDermal papillae
An object of the present invention is to promote hair growth or hair regeneration by promoting formation and / or regeneration of hair follicles. A method is provided for promoting formation and / or regeneration of hair follicles, comprising maintaining or increasing expression of one or a plurality of genes involved in cell adhesion in dermal papilla cells.
Owner:SHISEIDO CO LTD
Brain-protective agent
InactiveUS20040063614A1Efficient managementNervous disorderPeptide/protein ingredientsDiseaseApoptosis
A brain-protective agent containing an NF-kappaB decoy. In brain diseases, the brain can be particularly effectively protected against brain disorders (for example, cerebral vasospasm following a subrachnoidal hemorrhage and apoptosis of the nerve cells following a cerebrovasucular accident or serious head injury) caused by the undesired activation of cytokines or cell adhesion factors which are regulated by NF-kappaB by administering the brain-protective agent containing an NF-kappaB decoy, i.e., a compound antadonistic specifically to a nucleic acid to which NF-kappaB binds.
Owner:ANGES MG INC
Method for promoting hair growth or hair regeneration by maintaining or increasing expression of cell-adhesion factor
InactiveUS8334136B2Formation can be promotedPromote regenerationOrganic active ingredientsPeptide/protein ingredientsAdhesion processDermal papillae
An object of the present invention is to promote hair growth or hair regeneration by promoting formation and / or regeneration of hair follicles. A method is provided for promoting formation and / or regeneration of hair follicles, comprising maintaining or increasing expression of one or a plurality of genes involved in cell adhesion in dermal papilla cells.
Owner:SHISEIDO CO LTD
Hollow-fiber membrane and hollow-fiber module for cell culture
ActiveUS20180361325A1Safely and easily and efficiently culturingMembranesSemi-permeable membranesCulture cellHydrophobic polymer
An object of the present invention is to provide a hollow-fiber membrane which does not require a coating treatment with a cell adhesion factor or surface modification by an electron beam or the like and which is capable of adhering and culturing cells, and a method for culturing cells using the hollow-fiber membrane. A hollow-fiber membrane for cell culture which is to be used as a culture substrate for adhesive cells, in which the hollow-fiber membrane includes a hydrophobic polymer and a hydrophilic polymer, the content of the hydrophilic polymer in the whole hollow-fiber membrane is more than 0% by mass and less than 1% by mass, and the content of the hydrophilic polymer on a surface of the hollow-fiber membrane is more than 0% by mass and less than 10% by mass.
Owner:TOYO TOYOBO CO LTD
Endothelial cells engineered to express or overexpress fibulin-5 and a growth factor
The present invention relates to endothelial and smooth muscle cells genetically altered to express or over-express one or more cell adhesion factors. The invention further relates to cells genetically altered to express or over-express both cell proliferation growth factor(s) and cell adhesion factor(s). In addition, the present invention relates to nucleic acid constructs and nucleic acid construct systems that encode the cell adhesion and cell proliferation growth factors and that are used to transfect / transform the cells so that they can express the factors.
Owner:MULTI GENE VASCULAR SYST
Antibody chip kit for detecting cell adhesion molecules
ActiveCN106918707ALess specimenReduce sensitivityBiological material analysisBiological testingSpecific antibodyThroughput
The present invention relates to the technical field of biological detection, particularly to an antibody chip kit for detecting cell adhesion molecules. According to the antibody chip kit, a glass slide treated with a hydrophilic reagent containing alkyl glycoside as a solid-phase carrier, and the specific antibody mixture of a variety of cell adhesion factors is spotted onto the solid-phase carrier at a temperature of 20-26 DEG C under humidity of 35-45%. According to the present invention, with the kit, a variety of the cell adhesion factors commonly used in clinic can be detected, the defects of complex operation, single detection index, low sensitivity and the like in the prior art are overcome, and the kit has advantages of low cost, convenience, high sensitivity, accuracy, high throughput, less sample consumption, promotion and scalization in general laboratories, and the like.
Owner:RAYBIOTECH INC GUANGZHOU
Antibody chip capable simultaneously detecting various cell adhesion factors
The invention specifically discloses an antibody chip that can detect multiple cell adhesion factors simultaneously, including antibody chip, cell adhesion factor standard substance, cell adhesion factor standard substance mixture, biotin-labeled cell adhesion factor detection antibody, Biotin-labeled cell adhesion factor detection antibody cocktail; fluorescein-labeled streptavidin. The invention realizes that ten cell adhesion factors can be detected at one time, and the sensitivity of the detected cell adhesion factors can reach the ELISA detection sensitivity of a single factor. The antibody chip kit of the invention overcomes the defects of the prior art such as cumbersome operation, single detection index, low sensitivity, etc., and has the advantages of low cost, convenience, sensitivity, accuracy, high throughput, less sample consumption, and can be popularized and scaled in ordinary laboratories. advantages, etc.
Owner:梧州市兴能农业科技有限公司
Nucleic acid constructs encoding cell growth and adhesion factors and cells expressing them
The present invention relates to endothelial and smooth muscle cells genetically altered to express or over-express one or more cell adhesion factors. The invention further relates to cells genetically altered to express or over-express both cell proliferation growth factor(s) and cell adhesion factor(s). In addition, the present invention relates to nucleic acid constructs and nucleic acid construct systems that encode the cell adhesion and cell proliferation growth factors that are used to transfect / transform the cells so that they can express the factors.
Owner:MULTI GENE VASCULAR SYST
Method for detaching cultured cells, cell detachment device used in said method for detaching cultured cells, and incubator
InactiveUS8753855B2Bioreactor/fermenter combinationsBiological substance pretreatmentsShock waveCarbon nanotube
Disclosed is a method for detaching cultured cells that can selectively detach cultured adhered cells. The scaffold to which the cells adhere is configured from at least a cell adhesion factor containing carbon nanotubes, and by means of radiating laser light in a spot shape on the scaffold in a region where cells are adhered, a shock wave is generated by the heat arising by means of the photothermal conversion of the carbon nanotubes, and by means of this shock wave, the cells are caused to be in a non-adhered state.
Owner:JAPAN SCI & TECH CORP
Polymer suitable for use in cell culture
ActiveUS10266809B2Easy to collectSufficient bindingArtificial cell constructsPeptidesCarboxylic acidAlkyne
Owner:STICHTING RADBOUD UNIVERSITAIR MEDISCH CENT
Method for photochemical cross-linking cell adhesion peptides on chitosan hydroxyl
The invention relates to a method for photochemical cross-linking cell adhesion peptides on chitosan hydroxyl, namely a group selectively grafts the cell adhesion peptides on chitosan molecules. In particular, bifunctional photosensitive crosslinker sulfo-SANPAH is utilized to modify hydrophilic polypeptide GRGDY containing cell adhesion factors RGD on the chitosan hydroxyl to increase the water solubility and the targeting of a material, while amidogen is retained and tedious amino protection, a deprotection process and chemical toxicity caused by the same are avoided. The method adopts UV-activated photochemical cross-linking and has the advantages of mild reaction conditions and fast reaction rate, and the feature group of the crosslinker is beneficial to judging the conducting of grafting reaction. The targeted adhesion chitosan material synthesized by the method is suitable for preparing DNA and small RNA (siRNA, microRNA, shRNA, and the like.) targeted delivery systems, cell adhesion cultures, microencapsulated tissue cells for transplant, scaffolds for tissue engineering, and the like applied in the biomedical field under physiological conditions.
Owner:ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
Method for studying migration, proliferation and differentiation of brain endogenous neural stem cells
InactiveCN103940985ADisease diagnosisBiological testingGlial cell line-derived neurotrophic factorNeural cell
The invention relates to a method for studying migration, proliferation and differentiation of brain endogenous neural stem cells. The method comprises the following steps: (1) performing double-labeled immunofluorescent staining on an animal brain section by 5-bromndeoxyuridine and doublecortin, 5-bromndeoxyuridine and neuronal nuclei antigen as well as 5-bromndeoxyuridine and glial cell line-derived neurotrophic factor, and single-labeled immunofluorescent staining by sialyl neural cell adhesion molecules; and (2) carrying out statistic analysis on data and drawing by GraphPad Prism6.0 software, and performing one-way variance analysis on single-factor multi-group data of the 5-bromndeoxyuridine positive cells, wherein the animal brain section is subjected to the following treatment: stereotactically injecting a 6-hydroxydopamine solution into a corpus striatum of the right side of the brain, injecting a PSA (prostate-specific antigen) specificity lyase stock solution into the lateral ventricle, and then injecting a 5-bromndeoxyuridine solution in the abdominal cavity. An effective method for studying migration influence of the brain endogenous neural stem cells is provided.
Owner:CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Pharmaceutical Composition for Preventing or Treating Osteoporosis Which Comprises Neuropeptide Y as Active Ingredient
ActiveUS20160243198A1Reduce expressionGood effectPeptide/protein ingredientsSkeletal disorderOsteoblastDense Core Vesicles
The present invention relates to a pharmaceutical composition for preventing or treating osteoporosis which comprises neuropeptide Y as an active ingredient. The neuropeptide Y according to the present invention reduces the expression of a cell adhesion factor in osteoblasts in which a Y1 receptor is present, and consequently an effect of releasing a haematopoietic stem cell from bone marrow into the blood is excellent. When a haematopoietic stem cell is released into the blood, the number of osteoclasts which induce an osteoporotic lesion by differentiation from the haematopoietic stem cell decreases, and thus progression of a bone erosion caused by osteoclasts can be prevented. Accordingly, the neuropeptide Y which is an active ingredient of the composition of the present invention is useful as a therapeutic agent for osteoporosis.
Owner:KYUNGPOOK NAT UNIV IND ACADEMIC COOP FOUND
Method for targeted inhibition of infiltration and transfer of glioma cells and application of method
InactiveCN102643785AOvercoming non-selectivityImproved prognosisGenetic material ingredientsAntibody ingredientsCell adhesionIn vivo
The invention discloses a method for targeted inhibition of infiltration and transfer of glioma cells, which includes targeted inhibition of JAM2 (junctional adhesion molecules 2) of the glioma cells. Experiments show that cell proliferative activity of the glioma cells can be inhibited in vivo by 30% by inhibition on the JAM2 of the glioma cells, cell migration activity can be inhibited by more than 80%. The method has superb clinical application prospect, and by the method, non-specificity of existing drug therapy on glioma cell cases, the defects in surgical therapy, radiotherapy, chemotherapy and endocrine therapy are overcome, and the range of targeted therapy is widened.
Owner:ZHEJIANG FOCUSGEN BIOENG
Application of osteopontin as target molecule in regulation of intestinal flora colonization
ActiveCN111366733AInhibition of colonizationIncrease the number ofMicrobiological testing/measurementBiological material analysisMicrobiologyProbiotic bacteria
The invention discloses an application of osteopontin as a target molecule in regulation of intestinal flora colonization. Specifically, the method comprises the following steps that OPN gene expression is inhibited or combination of OPN and a cell receptor is blocked so as to increase the number of probiotics in intestinal tracts; blocking combination of the OPN and the cell receptor or inhibition of a Notch signal path can improve the adhesion effect of bacteria and intestinal epithelial cells. The method is advantaged in that the method proves that the osteopontin influences the adhesion effect between cells and the bacteria by inhibiting the expression of cell adhesion factors, and thereby colonization of flora in intestinal tracts is inhibited.
Owner:ZHEJIANG UNIV
Adsorbent for blood coagulation factor or cell adhesion factor and method for purifying the factor
An objective of the invention is to provide an adsorbent allowing purification of a blood coagulation factor or a cell adhesion factor under mild conditions, according to simple procedures, and at a low cost and safely while having a high affinity and a high resistance to deterioration, and a method for purifying the blood coagulation factor or the cell adhesion factor; a solution is to apply a polypeptide having peptide fragments represented by formula (1) as the adsorbent for the blood coagulation factor or the cell adhesion factor, and to purify the blood coagulation factor or the cell adhesion factor using the adsorbent:-(Pro-Hyp-Gly)n-Wherein, in formula (1), n is an integer from 2 to 9,000.
Owner:JNC CORP
Adsorbent for blood coagulation factor or cell adhesion factor and method for purifying the factor
An objective of the invention is to provide an adsorbent allowing purification of a blood coagulation factor or a cell adhesion factor under mild conditions, according to simple procedures, and at a low cost and safely while having a high affinity and a high resistance to deterioration, and a method for purifying the blood coagulation factor or the cell adhesion factor; a solution is to apply a polypeptide having peptide fragments represented by formula (1) as the adsorbent for the blood coagulation factor or the cell adhesion factor, and to purify the blood coagulation factor or the cell adhesion factor using the adsorbent:-(Pro-Hyp-Gly)n-Wherein, in formula (1), n is an integer from 2 to 9,000.
Owner:JNC CORP
Method for photochemical cross-linking cell adhesion peptides on chitosan hydroxyl
The invention relates to a method for photochemical cross-linking cell adhesion peptides on chitosan hydroxyl, namely a group selectively grafts the cell adhesion peptides on chitosan molecules. In particular, bifunctional photosensitive crosslinker sulfo-SANPAH is utilized to modify hydrophilic polypeptide GRGDY containing cell adhesion factors RGD on the chitosan hydroxyl to increase the water solubility and the targeting of a material, while amidogen is retained and tedious amino protection, a deprotection process and chemical toxicity caused by the same are avoided. The method adopts UV-activated photochemical cross-linking and has the advantages of mild reaction conditions and fast reaction rate, and the feature group of the crosslinker is beneficial to judging the conducting of grafting reaction. The targeted adhesion chitosan material synthesized by the method is suitable for preparing DNA and small RNA (siRNA, microRNA, shRNA, and the like.) targeted delivery systems, cell adhesion cultures, microencapsulated tissue cells for transplant, scaffolds for tissue engineering, and the like applied in the biomedical field under physiological conditions.
Owner:ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
Adsorbent, solid phase carrier and purification method
ActiveCN103130872AEliminate worriesAvoid modificationFactor VIIConnective tissue peptidesHigh resistancePurification methods
An adsorbent, a solid phase carrier and a purification method are provided. An objective of the invention is to provide an adsorbent allowing purification of a blood coagulation factor or a cell adhesion factor under mild conditions, according to simple procedures, and at a low cost and safely while having a high affinity and a high resistance to deterioration, and a method for purifying the blood coagulation factor or the cell adhesion factor; a solution is to apply a polypeptide having peptide fragments represented by formula (1) as the adsorbent for the blood coagulation factor or the cell adhesion factor, and to purify the blood coagulation factor or the cell adhesion factor using the adsorbent: -(Pro-Hyp-Gly)n- Wherein, in formula (1), n is an integer from 2 to 9,000.
Owner:JNC CORP
Polymer suitable for use in cell culture
ActiveUS20160137977A1Easy to collectSufficient bindingPeptidesArtificial cell constructs3D cell cultureBiotin
A process for making an oligo(alkylene glycol) functionalized co-polyisocyanopeptide, wherein the process includes the steps of: i) copolymerizing a first comonomer of an oligo(alkylene glycol) functionalized isocyanopeptide grafted with a linking group and a second comonomer of a non-grafted oligo(alkylene glycol) functionalized isocyanopeptide, wherein the molar ratio between the first comonomer and the second comonomer is 1:500 and 1:30 and ii) adding a reactant of a spacer unit and a cell adhesion factor to the copolymer obtained by step i), wherein the spacer unit is represented by general formula A-L-B, wherein the linking group and group A are chosen to react and form a first coupling and the cell adhesion factor and group B are chosen to react and form a second coupling, wherein the first coupling and the second coupling are independently selected from the group consisting of alkyne-azide coupling, dibenzocyclooctyne-azide coupling, oxanorbornmadiene-based-azide couplings, vinylsulphone-thiol coupling, maleimide-thiol coupling, methyl methacrylate-thiol coupling, ether coupling, thioether coupling, biotin-strepavidin coupling, amine-carboxylic acid resulting in amides linkages, alcohol-carboxylic acid coupling resulting in esters linkages and NHS-Ester (N-Hydroxysuccinimide ester)-amine coupling and wherein group L is a linear chain segment having 10-60 bonds between atoms selected from C, N, O and S in the main chain.
Owner:STICHTING RADBOUD UNIVERSITAIR MEDISCH CENT
Application of osteopontin as a target molecule in regulating intestinal flora colonization
ActiveCN111366733BInhibition of colonizationIncrease the number ofMicrobiological testing/measurementBiological material analysisBone bridgeCellular receptor
The invention discloses the application of osteopontin as a target molecule in regulating intestinal flora colonization; specifically: inhibiting the expression of OPN gene or blocking the combination of OPN and cell receptors, thereby increasing the number of probiotics in the intestinal tract; blocking OPN Binding to cellular receptors or inhibiting Notch signaling can enhance bacterial adhesion to intestinal epithelial cells. The present invention confirms that osteopontin affects the adhesion between cells and bacteria by inhibiting the expression of cell adhesion factors, thereby inhibiting the colonization of bacteria in the intestinal tract.
Owner:ZHEJIANG UNIV
Hollow-fiber membrane and hollow-fiber module for cell culture
ActiveUS10898863B2Safely and easily and efficiently culturingSemi-permeable membranesMembranesHollow fibreCulture cell
An object of the present invention is to provide a hollow-fiber membrane which does not require a coating treatment with a cell adhesion factor or surface modification by an electron beam or the like and which is capable of adhering and culturing cells, and a method for culturing cells using the hollow-fiber membrane. A hollow-fiber membrane for cell culture which is to be used as a culture substrate for adhesive cells, in which the hollow-fiber membrane includes a hydrophobic polymer and a hydrophilic polymer, the content of the hydrophilic polymer in the whole hollow-fiber membrane is more than 0% by mass and less than 1% by mass, and the content of the hydrophilic polymer on a surface of the hollow-fiber membrane is more than 0% by mass and less than 10% by mass.
Owner:TOYOBO CO LTD
Pharmaceutical composition for preventing or treating osteoporosis which comprises neuropeptide Y as active ingredient
ActiveUS9724394B2Reduce expressionGood effectPeptide/protein ingredientsSkeletal disorderOsteoblastDense Core Vesicles
The present invention relates to a pharmaceutical composition for preventing or treating osteoporosis which comprises neuropeptide Y as an active ingredient. The neuropeptide Y according to the present invention reduces the expression of a cell adhesion factor in osteoblasts in which a Y1 receptor is present, and consequently an effect of releasing a haematopoietic stem cell from bone marrow into the blood is excellent. When a haematopoietic stem cell is released into the blood, the number of osteoclasts which induce an osteoporotic lesion by differentiation from the haematopoietic stem cell decreases, and thus progression of a bone erosion caused by osteoclasts can be prevented. Accordingly, the neuropeptide Y which is an active ingredient of the composition of the present invention is useful as a therapeutic agent for osteoporosis.
Owner:KYUNGPOOK NAT UNIV IND ACADEMIC COOP FOUND
An antibody chip kit for detecting cell adhesion molecules
ActiveCN106918707BLess specimenReduce sensitivityBiological material analysisBiological testingSpecific antibodyThroughput
The present invention relates to the technical field of biological detection, particularly to an antibody chip kit for detecting cell adhesion molecules. According to the antibody chip kit, a glass slide treated with a hydrophilic reagent containing alkyl glycoside as a solid-phase carrier, and the specific antibody mixture of a variety of cell adhesion factors is spotted onto the solid-phase carrier at a temperature of 20-26 DEG C under humidity of 35-45%. According to the present invention, with the kit, a variety of the cell adhesion factors commonly used in clinic can be detected, the defects of complex operation, single detection index, low sensitivity and the like in the prior art are overcome, and the kit has advantages of low cost, convenience, high sensitivity, accuracy, high throughput, less sample consumption, promotion and scalization in general laboratories, and the like.
Owner:RAYBIOTECH INC GUANGZHOU
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