The invention relates to a
rapid detection kit and a detection method for
bombyx mori nuclear polyhydrosis
virus, specifically relates to a quick and convenient detection kit for detecting the
bombyx mori nuclear polyhydrosis
virus by combining the loop-mediated isothermal amplification technology and the
nucleic acid detection lateral
chromatography test paper, and a detection method of the detection kit, and belongs to the technical field of viral
epidemic disease diagnosis. The detection kit is characterized by comprising a BmNPVLAMP pre-reaction liquid, wherein the desired primer and probe are designed according to the conserved region of the genomic sequence of the
bombyx mori nuclear polyhydrosis
virus published by
GenBank; the kit is capable of effectively and quickly detecting the
bombyx mori nuclear polyhydrosis virus; the steps of virus
DNA extraction and BmNPVLAMP
reaction system amplification are performed, and after the reaction ends, the result is judged by use of the quick
nucleic acid detection test paper to obtain that the BmNPV virus
DNA is efficiently and specifically amplified; the detection method is simple, convenient and quick, good in specificity, and high in sensitivity, and is suitable for farmers to use daily and advantageous for diagnosing and preventing the
bombyx mori nuclear polyhydrosis virus
disease.