SNP molecular marker related to bombyx mori nuclear polyhedrosis virus resistance and application thereof

A nuclear polyhedron and molecular marker technology, applied in the fields of molecular biology and molecular breeding, can solve problems such as the accuracy is easily affected by the environment, low detection efficiency, and heavy workload, so as to improve breeding efficiency and reduce false positive rate , easy-to-operate effect

Pending Publication Date: 2022-08-05
JIANGSU UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0005] Most of the molecular markers linked to BmNPV disease resistance-related genes in silkworms discovered at present are RAPD markers or SSR markers. However, the identification of these two molecular markers for resistance genes is complicated, the detection efficiency is low, and the relationship with resistance traits is incomplete. Linkage, not suitable for molecular marker-assisted silkworm breeding
[0006] Studies based on genetic laws have shown that the BmNPV disease resistance traits of silkworm are controlled by two or more pairs of genes, and there is an additive effect; existing research results also show that molecular markers based on single resistance gene linkage cannot effectively identify BmNPV in silkworms disease resistance traits
At present, the breeding of silkworm BmNPV disease-resistant varieties mainly relies on the traditional phenotypic identification method, which has a large workload, a long cycle and the accuracy is easily affected by the environment.

Method used

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  • SNP molecular marker related to bombyx mori nuclear polyhedrosis virus resistance and application thereof
  • SNP molecular marker related to bombyx mori nuclear polyhedrosis virus resistance and application thereof
  • SNP molecular marker related to bombyx mori nuclear polyhedrosis virus resistance and application thereof

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Embodiment 1

[0066] Example 1. Development of silkworm BmNPV disease resistance SNP molecular marker panel

[0067] The development process of silkworm BmNPV disease resistance SNP molecular marker set is as follows: figure 1 , the SNP molecular marker group including Chr3-746 marker and Chr27-5071 marker is obtained by screening and verification, and the specific steps are as follows:

[0068] (1) Construction of genetic mapping population

[0069] The BmNPV-resistant silkworm line 'NB' was used as the male parent, and the BmNPV-susceptible silkworm line '306' was used as the female parent to obtain the F1 generation. The F1 generation and the silkworm line '306' were laterally crossed to obtain the BC1 generation. There were 152 lateral lines in total. Generation of individuals, at 3 years of age by feeding 10 8 The NPV virus at the concentration of BmNPV / mL divides the BC1 generation population into the resistant group (survival after adding the virus) and the susceptible group (death...

Embodiment 2

[0130] Example 2. Application of silkworm BmNPV disease resistance SNP molecular marker set in assisting silkworm breeding of BmNPV disease resistant strains

[0131] (1) For the molecular marker-assisted selection process, see Figure 8 ,details as follows:

[0132] Using the wings of silk moth of 'NB' strain as material, the genomic DNA was extracted, and the genotypes of Chr3-746 marker and Chr27-5071 marker were detected by KASP method, and Chr3-746 marker was selected as AA genotype and Chr27-5071 marker as GG genotype. Silkworm moth is used as the male parent, and the production variety with excellent economic traits is selected as the female parent for hybridization, and then it is continuously backcrossed with the recurrent parent with excellent economic traits to the 8th generation, and then self-crossed for more than 2 generations to select the moth area with non-segregated resistance traits for succession. generation. In order to ensure the accurate transmission o...

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Abstract

The invention belongs to the technical field of molecular biology and molecular breeding, and particularly relates to an SNP molecular marker related to bombyx mori nuclear polyhedrosis virus (BmNPV) resistance and application of the SNP molecular marker. The SNP molecular marker comprises a combination of a Chr3-746 marker and a Chr27-5071 marker, and the combination of the Chr3-746 marker and the The Chr3-746 marker is located at the 14951064th site of a No.3 chromosome sequence of a silkworm p50T genome, and the base polymorphism is G / A; the Chr27-5071 marker is located at the 9462596th site of a chromosome sequence 27 of a silkworm p50T genome, and the base polymorphism of the Chr27-5071 marker is C / G. The SNP molecular marker can effectively distinguish BmNPV-resistant silkworms, reduce the false positive rate of detection, improve the accuracy rate, realize rapid and high-throughput screening, solve the technical problem of traditional breeding of silkworms and accelerate breeding of BmNPV-resistant varieties of silkworms.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and molecular breeding, and particularly relates to a SNP molecular marker related to the resistance of Bombyx mori nuclear polyhedrosis virus and its application. Background technique [0002] Bombyx mori is an important economic insect for producing silkworm cocoons. Blood pus disease caused by Bombyx mori nuclear polyhedrosis virus (BmNPV) is a major infectious disease in the world sericulture industry. cause more than 60% of the total loss. Existing studies have proved that there are major control genes for the resistance of silkworm to BmNPV disease; experimental studies on the resistance of silkworm BmNPV and its genetic laws show that there are differences in resistance among silkworm strains, and resistance is incompletely dominant to susceptibility, and resistance to BmNPV is not completely dominant. Sex is controlled by more than two pairs of genes. Breeding new varieties res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02A50/30
Inventor 吕鹏张茹松潘晔刘晓勇姚勤陈克平
Owner JIANGSU UNIV
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