30 results about "Biomolecular complex" patented technology

The invention provides methods for detecting an interaction between an analyte and a biomolecule. The method comprises separating at least one biomolecule according to its isoelectric point in the presence of a given analyte and detecting an interaction between the analyte and a biomolecule using fluorescence anisotropy. The method may further comprise collecting the analyte-biomolecule complex and analyzing the biomolecule.

The invention provides a method for identifying a key module and a key node by fusing multiple methods based on a topological structure of a biomolecular complex network such as a protein interaction network, a gene expression regulation network, a biological metabolism network, an epigenetic network, a phenotype network or a signal conduction network. The method comprises the following main steps of performing module division on the network based on the biomolecular network, and comprehensively and quantitatively identifying the key module and the key node from multiple angles by adopting multiple measurement methods based on the topological structure of the network for the network or each module.

In order to solve the problems that some biological products are poor in stability, cannot be stored at normal temperature, cannot be well stored easily and are not convenient to use in emergency circumstances such as a disaster, the invention relates to a porous frame material creatively applied to biomolecule storage, and the stability and other performances are improved. The porous frame material and biomolecules are used for forming a porous frame material / biomolecule compound, the biomolecule is wrapped, and therefore the protection effect is achieved. On the premise of keeping the biomolecule activity, the system can achieve efficient separation and recycling of porous materials and biomolecules. While a biological product protecting system is developed, various performances are comprehensively detected and evaluated, the technical purposes of synthesis, storage, release and the like are solved, the good technical effect is achieved, the biomolecules can be effectively protected,and the material is used for storing a biological agent and preparing novel preparations.

The present application pertains to improved methods of detecting biomolecules in a biological sample (or system), In particular, embodiments discussed herein allow for the detection of biomolecule complexes. The embodiments enable for the first time the elucidation of the significance of biomolecule complexes for certain disease states, which in turn enables the diagnosis of disease states based on the identity and complexing level of a biomolecule complex in a particular biological sample.

The invention provides methods for detecting an interaction between an analyte and a biomolecule. The method comprises separating at least one biomolecule according to its isoelectric point in the presence of a given analyte and detecting an interaction between the analyte and a biomolecule using fluorescence anisotropy. The method may further comprise collecting the analyte-biomolecule complex and analyzing the biomolecule.

A novel composite biological agent based on a porous frame material, comprising porous frame materials and biomolecules. The porous frame materials cover a biological product, wherein the porous frame materials are metal-organic frame material (MOFs), covalent organic frame materials (COFs), and hydrogen-bonding organic frame materials (HOFs), and the biomolecules are any one or a combination of antibodies, enzymes, peptides, vaccines, nucleotides, and virus species. The composite biological agent uses the porous frame materials and biomolecules to form a porous frame material / biomolecule complex, and the biomolecules are coated to achieve the protection effect. Under the premise of remaining biomolecule activity, the system can achieve efficient separation and recovery of the porous materials and the biomolecules, so that the technical problems of synthesis, storage, release, etc. are solved, a good technical effect is achieved, and the biomolecules are effectively protected. The system is applied to the storage and transportation of biological agents and preparation of novel agents.

The disclosure relates to devices and instruments for detecting and individually analyzing biomolecules, biomolecular complexes and biomolecules with ligands attached thereon.

The present invention relates to complexes comprising one or more markers and one or more biomolecules for use as a contrast agent in positron emission tomography based imaging. The complexes according to the present invention preferably accumulate in a target compartment, such as the kidney. The present invention further relates to methods for generating the complexes and methods for using the complexes such as for evaluation of different aspects of kidney functionality e.g. for calculation of total and / or regional glomerular filtration rate in the kidney without the need for sampling blood or urine.

The present invention provides a biomolecular composite which is a ghost cell which has been emptied of all or substantially all of the cytosolic contents of the cell including the nucleus and nuclear contents, preferably further having been fused with exogenous amphipathic molecules, wherein said composite has a hydrodynamic diameter of less than 6 μm. The biomolecular composite may contain a cargo molecule and be capable of targeting a cell or tissue. Also provided are methods of preparing ghost cells, methods of preparing biomolecular composites and methods for their use.

Hg2+ and Ag+ are two environmental pollutants which have serious toxicity and are widely distributed, the Hg2+ and the Ag+ can seriously threaten the environment and human beings even under low concentration, and serious and permanent damage is caused. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting Hg2+ and Ag+ is of great importance to the fields of environmental monitoring, food safety and the like. The invention discloses a nanocapsule-nucleic acid biomolecule compound capable of simultaneously detecting Hg2+ and Ag+ and based on an exonuclease cyclic amplification technology and a preparation method of the nanocapsule-nucleic acid biomolecule compound. The Hg2+ and the Ag+ can be simultaneously detected by adopting the nanocapsule-nucleic acid biomolecule compound provided by the invention, and on the premise of ensuring high specificity, the detection sensitivity is obviously improved; and meanwhile, a wider linear detection range for Hg2+ and Ag+ is further obtained.

The present invention relates to a photothermal preparation based on biliverdin, including: biliverdin, biliverdin gel, biliverdin-metal chelate, biliverdin-biomolecular complex, biliverdin-biomolecule-metal functional assembly. The prepared photothermal preparation has the following advantages: 1) near-infrared absorption and good tissue penetration depth; 2) high photothermal conversion efficiency; 3) biocompatibility and clear metabolic mechanism. The preparation of this series of biliverdin-based photothermal preparations is of great significance for the development of multifunctional phototherapeutic drugs, and has great application potential in tumors, skin diseases (such as pigmentary diseases), and vascular diseases.

The invention relates to compositions of DOTA derivative compounds, lanthanoid-DOTA derivative molecular complex, and lanthanoid-complex encapsulated solid lipid particles or capsules, and methods of making and using the compositions. The solid lipid particles or capsules contain micelle cores stabilized by a hyperbranched polymer shell based from a crosslinked DOTA derivative compound or crosslinked lanthanoid-DOTA derivative complex. These solid lipid particles or capsules can be used in various applications, such as contrast agents or drug delivery vehicles.

Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and / or at least one molecular weight standard.

Hg2+ and Ag+ are two environmental pollutants which have serious toxicity and are widely distributed, the Hg2+ and the Ag+ can seriously threaten the environment and human beings even under low concentration, and serious and permanent damage is caused. The standard of drinking water is strictly limited by the World Health Organization. Therefore, establishment of an efficient and sensitive analysis method capable of simultaneously detecting Hg2+ and Ag+ is of great importance to the fields of environmental monitoring, food safety and the like. The invention provides a method for simultaneously detecting Hg2+ and Ag+ based on single excitation, the method utilizes a single excitation technology and adopts a nanocapsule-nucleic acid biomolecule compound based on an exonuclease cyclic amplification technology, not only can realize simultaneous detection of Hg2+ and Ag+, but also obviously improves the detection sensitivity on the premise of ensuring high specificity, and meanwhile, obtains a wider linear detection range on Hg2+ and Ag+.

The subject matter of the invention is a method for conjugation of free thiol group(s) containing biomolecules, leading to the biomolecular complex formation, comprising a reaction to connect biomolecules using a gold-donor agent in which a -S-Au-S- bond is formed, characterised in that a gold-donor agent is halogen(triarylphosphine)gold (I). The subject matter of the invention is also the use ofhalogen(triarylphosphine)gold (I) molecules as the gold-donor agent in the method of biomolecular complex formation.

The invention discloses a method of conjugating a substrate comprising exchanging a counterion bound to a biomolecule with a lipophilic counterion to form a biomolecular complex, dispersing the biomolecular complex in a non-aqueous solvent, and The biomolecular complex is coupled to the substrate in the presence of an aqueous solvent. The substrate or solid support is a polymer particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counterion is tetrabutylammonium ion, and the non-aqueous solvent is N-methylpyrrolidone (NMP). Oligonucleotide-coated particles are used in nucleic acid isolation or nucleic acid sequencing reactions.