40results about How to "Meet the needs of experimental research" patented technology

The invention discloses a bayberry orchard Drosophila elanogaster artificial breeding method, and a method for breeding parasitic wasp with the same, belonging to artificial breeding technology of natural enemy insects. The method successively propagates Pachycrepoideus vindemmiae capable of controlling bayberry orchard Drosophila elanogaster through the steps of Drosophila elanogaster breeding to obtain pupae, egg laying of Pachycrepoideus vindemmiae on the pupae, development and eclosion of parasitic wasp, and adult wasp breeding. The method is simple and convenient, and is especially suitable for large-scale indoor breeding of Pachycrepoideus vindemmiae to give large amount of wasps for test or release in bayberry orchard. Non-professional breeding personnel can grasp operation skill in short time. The breeding method has low land occupation and cost.

The invention belongs to the technical field of impeller machinery, and relates to a plane cascade experimental piece structure, in particular to a cantilever clearance-variable plane cascade experimental device. According to the device, a movable blade fixing plate and a movable blade fixing end plate are installed on a cascade side plate left grid plate, so that a novel clearance-variable planecascade experiment grid plate structure is formed, a single-side cantilever blade can be fixed, moreover, the position between the blade fixing plate and the left grid plate can be adjusted through common adjustment of a fixing bolt and a jacking bolt, then the change of the size of a clearance can be carried out, the clearance adjustment of a cantilever blade in the plane cascade is realized, theexperiment research requirements of the blade top clearance change and the blade top modification of a gas compressor can be completely met, and the important significance is achieved for the performance research of the gas compressor.

The invention discloses a testing fire and smoke grain high-speed sampling method and device, which is provided with a fuel gas interface on a drop burning platform connected with the fuel gas supplying pipeline. The fuel gas interface on the burning platform can be connected with nozzles of different models and an electricity heating zone is arranged at the surrounding zone around the fuel gas interface; or a liquid burning instrument is arranged on the burning platform, which achieves liquid burning fire of different scale through changing the side of a liquid burning container to conduct the corresponding burning study; at the surrounding zone of the heating zone, a ring-shaped filtering net is arranged to realize the stable controlling to burning; at one side of the heating zone or flame zone, a collecting piece is extended into the flame zone, and the collecting piece extends into the heating zone or the different heights or zones above the flame zone. By recording the position parameter of the collecting piece, the invention is beneficial for conducting regulated and repeatable burning experiment study.

The invention relates to a method for improving the parasitic rate of parasitic wasps to whitefly MED cryptic species, the method comprising the following steps: (1) cultivation of host plants; (2) breeding of host whitefly nymphs; (3) parasitic wasps (4) Cultivation, preservation and training of bank plants before release; (5) Field release of bank plants. The invention provides a method for improving the parasitic efficiency of the bemisia tabaci after the parasitoid bees are released from bank plants outdoors, establishes a stable field population, and satisfies the needs of efficiently controlling the bemisia tabaci of field crops.

The invention provides dynamic simulation device and method thereof of a super and extra high voltage controllable magnetic control shunt reactor, wherein the dynamic simulation device is capable of accurately simulating magnetic control shunt reactors with different capacities in 500kV, 750kV and 1,000kV power transmission systems. A simulation body consists of three single-phase magnetic control reactors; each single-phase magnetic control reactor is in a three-column structure; two beside core columns are working iron cores; a main alternating current winding and a direct current control coil are wound on each working iron core; and main alternating current windings on the two iron core columns are connected in parallel and connected to a power grid. Control windings are separated fromthe main windings so as to ensure the safety and the reliability of the device during work. The main windings are provided with twelve turn-to-turn short circuit taps which can respectively simulate 1-25 percent of turn-to-turn short circuit faults from the high-voltage side and the neutral point side; and the control windings are provided with seven turn-to-turn short circuit taps which can simulate 1-25 percent of turn-to-turn short circuit faults from the neutral point side. The body of the device is provided with a current transformer and a voltage transformer on a primary side, which areused for meeting the requirement for protecting a reactor body in a dynamic simulation test.

The invention relates to the field of insect breeding, and specifically discloses an indoor large-scale breeding method for Liriomyza longina, comprising the following steps: placing the eclosion Liriomyza longina in a beekeeping cage; Put the inoculation boxes of instar Bactrocera dorsalis larvae and larval feed into the beekeeping cages for inoculation; continue to raise the Bactrocera dorsalis larvae in the replaced inoculation boxes, and move them to the pupation box when the larvae develop to the age of 7 days Let the larvae pupate in the middle, and collect the remaining unemerged pupae within 10 to 14 days of pupation, which is the parasitic pupa; place the parasitic pupae in the beekeeping cage until the Liriomyza spp. eclosion emerges, and then the cocoon of Liriomyza spp. bee. The indoor large-scale breeding method of Liriomyza longina of the present invention is simple and easy, can be operated indoors, and can reproduce Liriomyza longtail on a large scale, realizes uninterrupted reproduction of the Liriomyza long-tailed throughout the year, and can be planted to melons and fruits The area provides a large number of insect sources for the control of Bactrocera dorsalis, which is harmful to melon and fruit crops.

The invention relates to methods of obtaining mesenchymal stem cells and is to provide a method of high-effectively obtaining mesenchymal stem cells from compact bone of mice. The method of high-effectively obtaining mesenchymal stem cells of mice includes the steps of: separating mesenchymal stem cells of mice, performing primary culture, and performing amplification culture to the mesenchymal stem cells of mice. The method greatly reduces the time of cell culture and can greatly increase quantity of produced cells, and is less in pollution of non-MSCs adherent cells. The invention, for the first time, discloses a multi-time bone sheet adhesion culture method, which can increase the yield of cells by 3-5 times. Through systematic researching on biological characteristics, such as growth status, surface markers, multidirectional differentiative potential and the like, of the MSCs from the compact bone of mice, it is proved that after 10 times of amplification culture in vitro, the cells still have strong characters of stem cells and can satisfy demands in experiment and researching. The method can quickly and high-effectively produce the stem cells. The obtained stem cells has high proliferation and growth speed and high differentiative potential.

The invention discloses a multi-field coupled in-vitro cell experimental device and a method. The device includes a container internally equipped with a thermal insulation interlayer and a magnetic stirrer, a flange cover provided with a plurality of holes on the surface, and a multifunctional sample stand. The upper end and the lower end of the container are respectively provided with a water inlet and a water outlet. The water outlet is provided with a ball valve. The magnetic stirrer is used for balancing the water bath temperature. The flange cover is connected to the top of the container to form a structure able to open or close the container. A porous cell culture plate-carrying electromagnetic field generation device and / or a single porous cell culture plate can be placed on the sample stand, which is placed in the container during experiment. The method comprises: putting the porous cell culture plate-carrying electromagnetic field generation device on the sample stand, then placing the sample stand into the container, and controlling the atmosphere, temperature and humidity conditions required by cell culture. The multi-field coupled in-vitro cell experimental device provided in the invention has the advantages of simple structure, low cost, convenient operation and multiple functions, and can meet the need of performing multiple groups of experiments at the same time.

A bacterium for degrading dicoumaric acid glucoside and its breeding method and application: including step 1, extracting bacteria from Eupatoria adenophorum invasion plots, culturing and purifying them, and step 2, carrying out molecular identification of isolated bacterial single colonies; Step 3. Degradation test of dicoumaric acid glucoside. This bacterium has a strong effect of degrading dicoumaric acid glucoside. When applied to the soil invaded by Eupatorium adenophorum, it can greatly alleviate the damage caused by Eupatorium adenophorum. The allelopathy of native plants can promote the recovery of native plants in Eupatorium adenophorum invasion.

The invention relates to a 1600kV / 50mA outdoor mobile direct current voltage generator which comprises a direct current voltage generator body, a charging voltage regulation device and a computer measuring and controlling system; the direct current voltage generator body adopts a five-stage voltage doubling rectifying circuit with symmetrical single sides, the complete set of the test units adopts a tower type combined structure and a container transportation method as well as the modular design. A direct-current voltage generator body module, the charging voltage regulation device, a computer measuring and controlling system operating platform are respectively provided with hoisting devices, and the hoisting devices can be moved on a transport vehicle; the invention utilizes the 50Hz power frequency power supply as the rectifier source to obtain the direct-current voltage, the quality of the direct-current power supply is good, the capacity is large, and the demands of experimental investigation of the extra-high voltage direct current transmission project can be met; and due to the adoption of the modular design, the rapid assembly and disassembly can be realized on site, and the polarity can be changed according to the complete set of the test units with required testing voltage.

The invention relates to an equipment for producing cheese in a laboratory, comprising a water bath box and two cheese troughs located in the water bath box, each cheese trough is provided with a cutter and a stirrer driven by a motor, and a water bath box is provided with a cutter and a stirrer. The electric heating pipe connected with the program-controlled digital instrument is provided with a water level switch and a temperature sensor connected with the program-controlled digital instrument on its side wall. The equipment accurately controls the temperature change curve through programming, and replaces manual operation with an automatic stirring device; one machine and two tanks are convenient for comparison experiments; the tank capacity is small, which reduces labor intensity and makes a large number of experiments possible, with high precision and high degree of automation , with strong repeatability. The price of the cheese producing equipment of the invention is far lower than that of similar foreign products, which is convenient for popularization and application.