The invention discloses an auxiliary operating floor for intravenous injection of rats. The auxiliary operating floor comprises a base, and a support rod is vertically and fixedly disposed on one sideof the upper end surface of the base. A movable ring is connected to the outer portion of the top end of the support rod in a sleeving mode, and the movable ring is internally provided with a limiting screw by forming a through hole. A fixing rod is fixedly disposed on one side of the movable ring, and a motion bar is connected to one end, away from the movable ring, of the fixing rod. A fastening screw is installed at one end of the motion bar by forming a screw hole, and the motion bar is movably connected with the fixing rod through the fastening screw. A regulating ring is connected to one end, away from the support rod, of the motion bar in a screw thread fit mode, and a magnifying lens is arranged at one end, away from the support rod, of the adjusting ring. An oblique support is fixedly arranged on one side, away from the support rod, of the upper end surface of the base. According to the auxiliary operating floor for the intravenous injection of the rats, the operating floor is closely connected with an injection device, the caudal vein injection work can be performed by a single person, the experimental efficiency is high, and injection is accurate; and at the same time,the oblique support enables an operation panel to be tilted at a certain angle to conform to the human engineering, and the injection process is easier.