41results about How to "Large amount of detection" patented technology

The invention relates to a fluorescent polymerase chain reaction (PCR) primer, a probe and a method for detecting the whole group of hemolytic streptococcus, belonging to the field of inspection and quarantine. In the primer, hemolysin S of A, C and G group hemolytic streptococcus is used as a target gene site to design a fluorescent PCR primer and a probe and establish a fluorescent PCR detection method for the whole group of the hemolytic streptococcus. The fluorescent PCR primer, the probe and the method are mainly used for detecting the hemolytic streptococcus.

The invention discloses a fluorescent quantitative RT-PCR detection reagent for a bean pod mottle virus, a preparation method thereof and application of the fluorescent quantitative RT-PCR detection reagent for the bean pod mottle virus. The fluorescent quantitative RT-PCR detection reagent for the bean pod mottle virus is synthesized through elaborate design by selecting conservative CP encodinggene fragments between various strains taking special bean pod mottle virus as target sequences, and comprises a pair of special primers and a special fluorescent probe, wherein the length of an amplified target fragment is 98 bp; and the sequences of the primers and the probe are as follows: the sequences of the primers are BPMV TF: 5'-ttgatggcacaggaggaaatt-3' and BPMV TR: 5'-acatgcattgctgtagcttgag-3', and the sequence of the probe is 5'-FAM-tgtcttctagtgcaagtga-MGB-3'. The fluorescent quantitative RT-PCR detection reagent for the bean pod mottle virus overcomes the defects of time consumption, easy pollution, requirement of electrophoretic detection after amplification and so on. of general RT-PCR, can quickly and accurately perform qualitative and quantitative detection on BPMV in a sample, and has the advantages of simplicity, easy operation, directviewing result, high sensitivity, good repeatability and so on.

The invention relates to a method for estimating the number of vehicles at a road segment based on a travel time distribution rule. The method comprises the steps: (1), pre-building of a travel time distribution model of different turning directions and time periods: extracting the travel time samples of a road segment m at the same date in one historical cycle for classification to obtain a travel time sample set, and building the travel time distribution model of different turning directions and time periods; (2), the real-time estimation of the number of online vehicles at the road segment:collecting the data of identity detection equipment at a downstream intersection of the same road segment m in real time; calculating the moment when a vehicle enters the road segment based on the travel time, which is detected by the estimation of the above model and is employed by the vehicle for passing through the road segment m, through the moment when the vehicle leaves the road segment m and the estimated travel time; judging whether the vehicle is located on the road segment m at the moment t or not: adding one to the number of vehicles if the vehicle is located on the road segment mat the moment t, or else performing no recording, and finally obtaining the number of vehicles on the road segment m at the moment t through accumulation. The method is good in implementation performance, is high in efficiency, is low in cost, and can be widely used for the estimation of the number of vehicles at road segment.

The invention relates to a method and a device for rapidly identifying a reproductive freezing vitrified solution. The method comprises the following steps: freezing a to-be-detected solution for 5-10seconds at the temperature of minus 70 DEG C to minus 90 DEG C, observing the appearance and the flow condition of the frozen solution, and judging whether the solution is the reproductive freezing vitrified solution; if the frozen solution shows a transparent flow condition, then the solution belongs to the reproductive freezing vitrified solution; and if the frozen solution is white and does not flow, then the solution does not belong to the reproductive freezing vitrified solution. Compared with the conventional detection method, the method has the advantages that the operation is simple,the method takes short time, and the detection can be carried out before reproductive freezing. Therefore, the reproductive freezing failure caused by an agent is avoided, and the unnecessary loss isreduced.

The invention provides a fault diagnosis method of an S4R serial-connection type sequence switch shunt regulator. The method includes following steps: analyzing the operating state of the S4R serial-connection type sequence switch shunt regulator in a no-fault condition; obtaining fault modes of a single-stage shunt regulating circuit in the fault condition of one or more switches through analysis; selecting detection points as solar cell sub-array voltages corresponding to multiple channels of shunt regulation circuits, and selecting a sampling frequency; selecting a sampling data time lengthto cover a complete switching cycle of the solar cell sub-array voltage; sampling the solar cell sub-array voltage corresponding to each stage of shunt regulation circuit, and sampling a storage battery pack voltage Vbat; searching a regulation stage; performing fault diagnosis according to the found regulation state; and continuing next round of sampling and diagnosis. By adopting the technicalscheme of the method, normal operation of the shunt regulator is not affected, the detection omission rate of faults is low, the fault detection rate is high, accurate positioning of the faults is realized, and the diagnosis speed is fast.