48results about How to "Increase the amount of effective data" patented technology

The invention discloses a high-throughput sequencing database construction technology capable of efficiently capturing the three-dimensional conformation of chromosomes of a frozen biological sample.The invention provides a sample pre-processing method suitable for Hi-C high-throughput sequencing library building. The sample pre-processing method includes the steps of freezing a biological sampleto be tested first, then increasing the temperature in a gradient mode, and then cross-linking and fixing. The FS-Hi-C technology is not only suitable for fresh samples, but also for frozen samples.The results show that the three-dimensional structure of chromatin of a frozen replacement sample and a fresh sample are highly similar, and the quality of a Hi-C library is improved. Compared with atraditional Hi-C pretreatment method, FS-Hi-C is highly consistent in chromatin interaction, A / B compartment and TAD and the like, the quality of the library constructed by the FS-Hi-C method is better, the storage capacity is increased, effective interaction data is more, and the repeated data generated by PCR amplification is reduced. The technology reduces the requirements for sample timeliness. The high-throughput sequencing database construction technology provides an improved method for the application of Hi-C in biology.

The invention discloses an SNP molecular marker for detecting heterogenous cfDNA, a detecting probe based on SNP molecular marker design and a chip, a detecting device for heterogenous cfDNA, a kit and a detecting method. The heterogenous cfDNA in a receptor after kidney transplantation is quantified by the detecting chip and the detecting device, a basis is provided for detection of kidney transplantation rejection, noninvasive early detection with high sensitivity and high specificity on kidney transplantation is realized, and a kidney transplantation patient can be monitored continuously. An SNP molecular marker comprises an SNP site of a drug metabolizing gene, mutation information of a medication gene is provided for a kidney transplantation rejection patient, and individualized medication is realized. The invention further provides a single molecular marker joint. The single molecular marker joint is used for constructing a sequencing library to effectively remove repeated data and error introduced randomly in sequencing and PCR processes, the sequencing background noises are reduced, and the detection accuracy is improved.

The present invention relates to the field of the Internet, and discloses a webpage browsing mode selection method and an electronic device so as to solve the technical problem in the prior art of a relatively small volume of valid data acquired by a home screen during a search based on a search engine. The method comprises: receiving a search operation; responding to the search operation and searching by means of a first search engine to obtain a first search result, wherein the first search result comprises M pieces of promotion information; determining whether a display width of a display interface of a browser is greater than a preset width; when the display width is greater than the preset width, displaying the first search result in a first display region of the display interface, wherein the first search result comprises the M pieces of promotion information; and displaying a second search result in a second display region of the display interface, wherein promotion information contained in the second search result is a proper subset of the M pieces of promotion information. The technical effect of increasing the volume of the valid data acquired by the home screen during the search based on the search engine is achieved.

The present invention relates to the field of the Internet and discloses a searching record display method and an electronic device so as to solve the technical problem that in prior art, when searching is carried out based on a search engine, the valid data amount obtained by a first screen is relatively small. The method comprises: receiving a search operation of carrying out network searching by a user through key information; then responding to the search operation to obtain a first search result under a first search engine; displaying the first search result on a first display area of a display interface of a browser, and displaying a second search result on a second display area of the display interface; determining whether a display width of the display interface is greater than a predetermined width; and when the display width is not greater than the predetermined width, displaying the keyword information corresponding to at least one searching record in the first search result and / or the second search result. The method and the electronic device which are provided by the present invention achieve the technical effect of increasing the valid data amount obtained by the first screen when searching is carried out based on the search engine.

The present invention provides a CAN bus-based data transmission method and device, the method comprising: combining the valid data carried in the nth data packet to be sent to the n+mth data packet to be sent to obtain the combined data; wherein , n and m are positive integers greater than zero; the merged data is checked to obtain check data; the check data is divided into m+1 pieces of sub-check data, and each block of sub-check data They are respectively carried in different data packets to be sent and sent to the receiving end through the CAN bus. The CAN bus-based data transmission method and device provided by the present invention can increase the amount of effective data that can be carried in each frame of data, thereby improving the utilization rate of data bandwidth.

The invention discloses a kit and a method for detecting 50 tumor-relevant hotspot mutation genes. According to the method, a capture method of 207 hotspot mutation regions of 50 relevant genes (including oncogenes and cancer suppressor genes) and a preparation method of a liquid-phase hybridization library used in capturing are researched aiming at oncology. The method particularly comprises the steps of selecting sequence information of 207 hotspot mutation regions, designing a PCR primer, preparing a biotin labeled liquid-phase hybridization library by virtue of a PCR method, hybridizing the biotin labeled liquid-phase hybridization library with a constructed target genome DNA library, and carrying out high-throughput sequencing and bioinformatics analysis on captured fragments, so as to obtain the mutation condition of nucleic acid sequence sites of a sample. By virtue of the method provided by the invention, high-quality sequencing data can be obtained, the relatively high capturing efficiency and the relatively good uniform capturing of the target sites can be realized, the disadvantages of low efficiency, poor uniformity and the like of solid phase hybridization are solved, and the sequencing cost is greatly lowered.

The invention discloses a hierarchical diagnosis and treatment evaluation method based on data mining. This method extracts basic patient information and diagnosis and treatment information from medical records, and associates them with disease type information and medical institution information, first cleans the data and fills in missing values, and classifies patients in different flow directions; then uses this data to calculate hierarchical diagnosis and treatment monitoring indicators; Through the GainRatioAttributeEva feature selection algorithm and the RIPPER classification algorithm, the behavior and characteristics of different patients are described. The present invention evaluates the graded diagnosis and treatment through qualitative indicators and quantitative indicators, and overcomes the shortcomings of the current graded diagnosis and treatment evaluation method that there are only quantitative indicators, the indicators need to be reported layer by layer, there are misreporting and random reports in the reporting process, and there is no reason behind the lack of tracking indicators. And use data mining technology to make graded diagnosis and treatment evaluation more timely and accurate.