58results about How to "Improve transcription efficiency" patented technology

The present invention concerns a method for modulating the enzymatic activity of an enzyme, wherein said enzyme interacts with BMP, said method comprising the step of administering or inducing Hsp70, or a functional fragment or variant thereof, in a form suitable for allowing interaction between BMP and Hsp70, or said functional fragment or variant thereof, and thereby modulating the enzymatic activity of an enzyme interacting with BMP.

The present invention relates in one embodiment to PAPP-A exosite(s) interactors such as antibodies which bind to a region comprising LNR3 of PAPP-A and efficiently inhibit proteolysis of IGFBP-4, but not -5. The region comprising LNR3 represents a substrate binding exosite, which can be targeted for selective proteolytic inhibition. Accordingly, the present invention relates in one embodiment to differential inhibition of natural protease substrates by exosite targeting.

The invention relates to an RT-PCR method for quantitatively detecting miRNA. The method is characterized in that Poly (A) tail adding and reverse transcription of miRNA are performed in the same reaction system, and S-Poly (T) primers are used to perform the reverse transcription of miRNA. The method has the advantages that in an S-Poly (T) Plus method, the Poly (A) tail adding and reverse transcription of miRNA are simultaneously performed in the same reaction system, simple operation is achieved, time is shortened, time for synthesizing cDNA is at least reduced by 0.5 hour, and high reverse transcription efficiency is achieved; an S/P miRsol RNA method and the S-Poly (T) Plus method are combined to build a technical system which is quite sensitive, efficient, simple, fast and cheap and capable of achieving extraction and detection; the technical system is especially suitable for detecting miRNA in biological fluid samples with low miRNA abundance.

The invention provides a meter reading method applied to a meter reading terminal. The method comprises the steps of selecting an identifier of a to-be-read electric meter from electric meter identifiers of a plurality of electric meters, fed back by a concentrator; and sending a read data acquisition instruction to the concentrator according to the identifier of the to-be-read electric meter, andreceiving read data fed back by the concentrator. The invention further provides a meter reading method applied to the concentrator. The method comprises the steps of sending the electric meter identifiers of the plurality of the electric meters associated with the concentrator to the meter reading terminal; and when the read data acquisition instruction sent by the meter reading terminal is received, acquiring the read data according to the read data acquisition instruction, and sending the read data to the meter reading terminal. Corresponding to the meter reading method, the invention further provides the meter reading terminal, the concentrator and a computer readable storage medium. By means of the technical scheme, the efficiency of electric meter reading can be improved.

The invention relates to the field of biotechnology and discloses a high-proliferation specific recombination eucaryotic cell promoter PCNATAI; an eucaryotic cell operating system PCNATAI-LoxP-stop-LoxP taking a PCNATAI as a core transcription initiator and a LoxP-stop-LoxP as a transcriptional repression element; and a set of general scheme for eliminating the repression effect of the LoxP-stop-LoxP to the transcription process of the PCNATAI promoter through a Cre recombinase provided by a tumor cell specific dependent model trans form and enabling the downstream exogenous tumor suppressor gene or the suicide gene to be effectively and specifically expressed in the malignant tumor cell in a tumor cell specific pattern and a proliferation specific pattern. The scheme not only can remarkably improve the tumor inhibition rate during the gene therapy of the malignant tumor or the expression efficiency of the suicide gene in the malignant tumor cell, but also can ensure the security of the gene therapy.

The invention discloses establishment of a self-activated Gal4/UAS system expression cassette capable of improving gene expression. The expression cassette with good improvement effect is obtained byoptimizing the number of UAS and the types of GAL 4 activation/binding domains; the expression cassette is started by the strong promoter CMV of the expression cassette and improves transcription andtranslation efficiency in virtue of a Gal4/UAS system, so the effects of automatic activation and cascade amplification are obtained; high improvement effect is maintained in 5 to 10 days after transfection, and highest expression activity is obtained in the eighth day, so it is proved that the expression cassette can improve the stability of gene expression; and improvement effect is about 9 times better in CHO cells specially producing recombinant proteins. Compared with conventional gene expression cassettes, the expression cassette provided by the invention is simple in structure and substantial in improvement effect, and provides an approach and novel method for non-virus transfection approaches of gene therapy and enhancement of the expression of target genes in production of recombinant proteins.

The invention provides a negative charge toner, method of manufacturing the same and image forming device using the same. The said toner comprises: toner agglomerate, hydrophobic additive with negative charge and with less work function than the toner agglomerate; hydrophobic additive with positive charge and with less work function than the toner agglomerate and for treating the surface of the toner agglomerate using material with positive charge. Therefore, the amount of fog toner on non-image portions is reduced, the transfer efficiency is further improved, the charging property is further stabilized, and the production of reverse transfer toner is further inhibited.

The invention relates to gene optimization of cholesterol dehydrogenase and high-efficiency expression thereof, belonging to the field of production of protein by utilizing recombinant DNA (deoxyribonucleic acid) technology. Particularly, the invention provides an optimized DNA sequence of cholesterol 7,8-dehydrogenase, which is suitable for a Escherichia coli expression system or a pichia pastoris expression system, and research on construction of engineering bacteria and the expression optimization technology of the cholesterol 7,8-dehydrogenase. The optimized gene is transferred into Escherichia coli and pichia pastoris to be expressed, and compared with the expression quantity of a non-optimized gene, the expression quantity of the optimized gene in the Escherichia coli and pichia pastoris is obviously improved.

We have developed a versatile plant viral vector system based on Alternanthera mosaic virus (AltMV), suitable for infection by agroinfiltration or in vivo T7 transcripts from the same clone; agroinfection is enhanced by coinfiltration of a T7 RNA polymerase construct. Variants adapted for efficient protein expression, or for virus-induced gene silencing (VIGS), are based on a specific amino acid substitution (L88P) in the triple gene block 1 (TGB1) protein affecting RNA silencing suppression. A bipartite delivery system developed for AltMV delivers replicase (RdRp) functions separately from movement and encapsidation (TGB and coat protein, CP) functions by agroinfiltration, resulting in precise recombination of RdRp and TGB-CP constructs in planta. The bipartite delivery system has potential for high throughput protein expression or VIGS with the appropriate TGB1 variant, for hosts including Nicotiana benthamiana and Arabidopsis thaliana. Equivalent TGB1 substitutions in other potexviruses also reduced RNA silencing suppression, demonstrated with Potato virus X.

The invention relates to a method for generating a transgenic organism. The invention also relates to a method for detecting and characterizing a genetic mutation in a transgenic organism. The invention further relates to a method for isolating a gene which is correlated with a phenotypic characteristic in a transgenic animal. The invention further relates to a method for isolating an exon in a transgenic animal. The invention also relates to a method for modulating the expression of a gene in an organism.

Novel zacrp3x2 polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed. Also disclosed are antibodies to the zacrp3x2 protein or fragments thereof.