The invention provides a carrier for in vitro transcription mRNA, and a construction method of the carrier, a method for obtaining mRNA by carrier transcription, and belongs to the technical fields ofmolecular cloning and in vitro transcription. A 5' UTR sequence, a target gene sequence, a 3' UTR sequence and a polyA sequence are connected to the carrier sequentially, and the carrier for in vitrotranscription of the mRNA is obtained. The carrier provided by the invention contains the 5' UTR sequence, the target gene sequence, the 3' UTR sequence and the polyA sequence, so that in vitro transcription of the mRNA can be effectively performed, addition of cap structure analogs is avoided, and the 3' UTR sequence can prolong the half life of translation protein, and the translation efficiency can be improved.