The invention belongs to the technical field of workpiece positioning, particularly relates to a positioning mechanism of air sensor press-fitting equipment, and provides the following scheme to solve the problems that existing positioning mechanisms mostly perform positioning and clamping manually, the tool efficiency is low, the application range of the positioning mechanisms is small, adjustment is inconvenient, and the positioning mechanisms cannot be conveniently used for different crowds. The positioning mechanism comprises a working plate, four supporting legs are fixedly installed at the bottom of the working plate, sliding blocks are installed on the four supporting legs in a sliding mode, universal wheels are fixedly connected to the sides of the four sliding blocks, two lifting plates are vertically installed at the top of the working plate in a sliding mode, the two lifting plates and the tops of the two corresponding sliding blocks are fixedly installed, and a lifting driving mechanism is arranged at the bottom of the working plate. The positioning mechanism is simple in structure and convenient to operate, workpieces can be automatically clamped and positioned, the tool efficiency is improved, the position of the positioning mechanism can be adjusted, the positioning device can be used for different crowds, and the application range is widened.