The invention relates to a medical examination layered chromogenic reaction device, and belongs to the technical field of medical apparatuses. The medical examination layered chromogenic reaction device comprises a device main body, the device main body is provided with side baffle plates, the side baffle plates are connected with an arc-shaped sealing plate through oriented movable shafts, fixedpoint fixing supports are arranged at the inner side of the arc-shaped sealing plate, the fixed point fixed supports are connected with telescoping sleeves through built-in rotating shafts, the telescoping sleeves are connected with telescopic support tubes, pedestal pasters are arranged at the lower sides of the telescopic support tubes, the arc-shaped sealing plate is provided with a middle port, a light transmission filter membrane is arranged in the middle port, an indicator light groove is arranged at the lower side of the middle port, and working indicator lights are arranged in the indicator light groove. The device has complete functions, is convenient to use, realizes a uniform chromogenic reaction without causing the component structure change of a sample solution in order to facilitate the observation of medical staffs, saves time and labor, is scientific, convenient, safe and highly-efficient, and greatly reduce the working difficulty of the medical staffs.