The invention discloses a
transplanting method of apple stock
tissue culture seedlings. The
transplanting method comprises the following steps: transferring the
tissue culture seedlings rooted in bottles in a proliferation culture chamber into a constant-temperature culture chamber with a temperature of 25 DEG C, raising the illumination intensity to 3-4 times of the original illumination intensity, and culturing the rooted
bottle seedlings at the illumination intensity for 7-8 days; gradually loosening
bottle caps, opening 1 / 4 of each
bottle cap every day, and opening each
bottle cap completely after 5 days, wherein the
humidity of the culture chamber is kept to be 50% or above; taking out the
tissue culture seedlings in the bottles, cleaning the residual culture medium at the roots by using clear water,
transplanting the tissue culture seedlings into a culture medium filled with
peat, carrying out film covering for keeping
moisture, carrying out covering with a sunshade net, removingthe sunshade net after culture is carried out in the constant-temperature chamber for 2 days, gradually removing the
moisture keeping film after 7 days, and removing the
moisture keeping film completely after two weeks; and when about 3 and 4 new leaves grow out 20 days after the transplanting, transferring the survived plants into a
sunlight greenhouse or a plastic
greenhouse for continuing growth. The transplanting method of the apple stock tissue culture seedlings disclosed by the invention not only ensures the transplanting
survival rate of the tissue culture seedlings, but also can realize the annual transplanting and
domestication of the tissue culture seedlings.