Tissue culture and rapid propagation method of Donghong actinidia fruits
A technology of tissue culture and rapid propagation of kiwifruit, which is applied in the field of tissue culture and rapid propagation of kiwifruit varieties, can solve problems such as the degree of healing of high and low affinity grafting interfaces, achieve good induction and adaptation, help hair roots, and avoid pollution sources.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0045] A kind of tissue culture fast propagation method of kiwi fruit Donghong is completed according to the following steps:
[0046] 1. Obtaining sterile seedlings:
[0047] At the end of November, the annual branches with robust growth and full axillary buds were cut from the oriental red mother tree, and cut into 30cm long branch sections with 10 buds in each section. The morphological upper end was sealed with parafilm, and the morphological lower end was soaked in 2‰ sucrose aqueous solution for indoor cultivation. , keep the ambient temperature at 25±2℃, keep the room clean, and replace the aqueous solution every 2 days. After two weeks, the axillary buds will sprout. In the sterilized empty bottle, disinfect the surface of 75% alcohol for 1 minute, pour out the alcohol, add 0.1% mercuric chloride in a mass-to-volume ratio for in-depth disinfection for 5 minutes, the mercury chloride solution and the external body are fully shaken to ensure thorough disinfection, and po...
Embodiment 2
[0057] A kind of tissue culture fast propagation method of kiwi fruit Donghong is completed according to the following steps:
[0058] 1. Obtaining sterile seedlings:
[0059] At the end of November, the annual branches with robust growth and full axillary buds were cut from the oriental red mother tree, and cut into 30cm long branch sections with 10 buds in each section. The morphological upper end was sealed with parafilm, and the morphological lower end was soaked in 2‰ sucrose aqueous solution for indoor cultivation. , keep the ambient temperature at 25±2℃, keep the room clean, and replace the aqueous solution every 2 days. After two weeks, the axillary buds will sprout. In the sterilized empty bottle, disinfect the surface of 75% alcohol for 1 minute, pour out the alcohol, add 0.1% mercuric chloride in a mass-to-volume ratio for in-depth disinfection for 4 minutes, the mercury chloride solution and the external body are fully shaken to ensure thorough disinfection, and po...
Embodiment 3
[0070] A kind of tissue culture fast propagation method of kiwi fruit Donghong is completed according to the following steps:
[0071] 1. Obtaining sterile seedlings:
[0072] At the end of November, the annual branches with robust growth and full axillary buds were cut from the oriental red mother tree, and cut into 30cm long branch sections with 10 buds in each section. The morphological upper end was sealed with parafilm, and the morphological lower end was soaked in 2‰ sucrose aqueous solution for indoor cultivation. , keep the ambient temperature at 25±2℃, keep the room clean, and replace the aqueous solution every 2 days. After two weeks, the axillary buds will sprout. In the sterilized empty bottle, disinfect the surface of 75% alcohol for 1 minute, pour out the alcohol, add 0.1% mercuric chloride in a mass-to-volume ratio for in-depth disinfection for 6 minutes, the mercury chloride solution and the external body are fully shaken to ensure thorough disinfection, and po...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com