Method of tissue culture and rapid propagation of oil plant white sandalwood
An oil plant, white sandalwood group technology, applied in the directions of botany equipment and methods, plant regeneration, gardening methods, etc., can solve the problems such as the inability to maintain the excellent traits of the female parent, prolong the white sandalwood seedling raising period, and the seedlings are prone to variation, etc. The effect of planting survival rate, ensuring the quality of seedlings and strong root system
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[0036] The method for tissue culture and rapid propagation of the oil-bearing plant white sandalwood comprises the following parts:
[0037] 1. Explant selection and pretreatment
[0038] Through fixed-point and regular surveys of wild white sandalwood resources for three consecutive years, an excellent individual plant with robust growth, no pests and diseases, high and stable fruit-bearing rate was selected as the mother plant.
[0039] In late November, after the fruit is ripe, it is picked and brought back to the laboratory. Remove the pulp from the seeds collected from the wild, soak them in warm water at 35°C with detergent for 30 minutes, rub the seeds clean, and rinse them under running water to ensure that the seed shells are free of grease.
[0040] Put the cleaned seeds on the ultra-clean workbench, wash them with sterile water for 2-3 times, then soak them in 75% alcohol solution for 30s, rinse them with sterile water for 2-3 times, and finally wash them with 0.1%...
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