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A kind of seedling hardening method of hybrid orchid tissue culture seedling

A technology for hybrid orchid and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of low survival rate, weak seedling growth, time-consuming and labor-intensive, etc. Good water permeability

Active Publication Date: 2022-03-01
GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used methods for transplanting survival rate of tissue culture seedlings still have time-consuming and labor-intensive methods, low survival rate, and weak growth of seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Select the vigorous and rooted Tonghaijian×Cymbidium hybrid orchid tissue culture seedlings, place them in the greenhouse, unscrew the bottle cap to allow the tissue culture seedlings to adapt to the environment, take out the seedlings in the bottle after 5 days, and wash the root culture medium. Soak the roots with tea bran extract with a volume concentration of 0.03-0.05% prepared by conventional methods for 10 minutes and dry them in the air until the roots turn white. The tea bran extract is obtained by soaking tea bran and warm water at 40°C in a weight ratio of 1:5 for 5 hours.

[0026] Wrap the roots of the seedlings with wet moss and soak them in the nutrient solution for 24 hours, the soaking depth is 1 / 3 of the root system; the nutrient solution is composed of the following concentration components: IAA 100 mg / L, ABT 1 150 mg / L, Paclobutrazol 10 mg / L, VB 12 10 mg / L, biotin 50 mg / L, NH 4 NO 3 400 mg / L, KH 2 PO 4 400 mg / L, K 2 SO 4 200 mg / L, MgSO 4 •7H...

Embodiment 2

[0032] Select the vigorously growing and rooted Cymbidium × Xiaoxuetin hybrid orchid tissue culture seedlings, place them in the greenhouse, unscrew the bottle cap to allow the tissue culture seedlings to adapt to the environment, take out the seedlings in the bottle after 5 days, and wash the root culture medium. The roots were soaked in tea bran extract with a volume concentration of 0.04% prepared by conventional methods for 10 minutes and dried in the air until the roots turned white. The tea bran extract is obtained by soaking tea bran and warm water at 40°C in a weight ratio of 1:5 for 5 hours

[0033] Wrap the roots of the seedlings with wet moss and soak them in the nutrient solution for 22 hours, the soaking depth is 1 / 3 of the root system; the nutrient solution is composed of the following concentration components: IAA 200 mg / L, ABT 1 130 mg / L, Paclobutrazol 12 mg / L, VB 12 8mg / L, biotin 80 mg / L, NH 4 NO 3 400 mg / L, KH 2 PO 4 400 mg / L, K 2 SO 4 200 mg / L, MgS...

Embodiment 3

[0039] Select the vigorous and rooted hybrid orchid seedlings of Wuzicui×Cymbidium grandis, place them in the greenhouse, unscrew the bottle cap to allow the seedlings to adapt to the environment, take out the seedlings in the bottle after 5 days, and wash the root culture medium , Soak the roots with tea bran extract with a volume concentration of 0.05% prepared by conventional methods for 10 minutes and dry them in the air until the roots turn white. The tea bran extract is obtained by soaking tea bran and warm water at 40°C in a weight ratio of 1:5 for 5 hours

[0040] Wrap the roots of the seedlings with wet moss and soak them in the nutrient solution for 20 hours, the soaking depth is 1 / 3 of the root system; the nutrient solution is composed of the following concentration components: IAA 300 mg / L, ABT 1 100 mg / L, Paclobutrazol 15 mg / L, VB 12 5mg / L, Biotin 100 mg / L, NH 4 NO 3 400 mg / L, KH 2 PO 4 400 mg / L, K 2 SO 4 200 mg / L, MgSO 4 •7H 2 O 400mg / L, Ca(NO 3 ) 2...

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Abstract

The invention discloses a method for hardening seedlings of hybrid orchid tissue culture seedlings, which includes the selection of tissue culture bottle seedlings, treatment of small seedlings, cultivation of small seedlings, transplanting of medium and large seedlings and post-transplanting management procedures, selection of vigorously growing hybrid orchids that have taken root Tissue culture seedlings, wash and disinfect their roots, soak them in nutrient solution for 20-24 hours, transplant them into a seedling container equipped with a seedling substrate and cultivate them for 4-6 months to obtain medium and large seedlings, and then transplant the medium and large seedlings on the cultivation site The seedlings were hardened in the cultivation medium, and the hybrid orchid seedlings were obtained through watering and fertilization and management after transplanting. The number of new leaves of orchid seedlings obtained by the present invention, the increment of new roots, the increment of average root length, the increment of plant height and other parameters have high values, which effectively improves the transplanting survival rate of orchid hybrid tissue culture seedlings, improves the quality of potted plants, and has a relatively high Good economic benefits.

Description

technical field [0001] The invention belongs to the technical field of flower cultivation, and relates to a seedling hardening method of hybrid orchid tissue culture seedlings. Background technique [0002] Orchid is a traditional famous flower in my country. It is a perennial herb with high ornamental and economic value unique to China. It is one of the top ten famous flowers in China. It is also called "Four Gentlemen" together with "plum, bamboo and chrysanthemum". [0003] Hybrid breeding is the main way to cultivate new varieties of orchids. Some interspecific and intergeneric hybrids have been obtained through artificial hybridization, and hybrids with different flower colors, flower shapes, fragrances, and high stress resistance have been bred. At present, the industrialization of orchid seedlings in test tubes and greenhouses in China has reached a large scale, and the commercial application of tissue rapid propagation is also booming all over the country. Transplan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G22/63A01G7/06A01G24/23A01G24/30C05G1/00C05G3/60C05G3/00
CPCA01G31/00A01G22/63A01G7/06A01G24/23A01G24/30C05B7/00C05C9/00C05G3/00C05F17/00C05F5/002
Inventor 罗清谢振兴丁丽琼陆祖正覃茜卢业飞丁长春黄歆怡宾振钧池昭锦谭奕为
Owner GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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