Technique for breeding pyrethrum of white flower
A technology of white-flowered pyrethrum and technology, which is applied in plant regeneration, horticulture, horticultural methods, etc., and can solve the problems of immature cultivation technology, poor practicability, and low efficiency
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[0024] Select the young shoots that grow about 10 days after the seeds germinate of the fine varieties of Pyrethrum chrysanthemum as explants. Compound disinfection method for disinfection and sterilization of explants: rinse the explants five times with distilled water, first sterilize with 2% sodium hypochlorite solution (add 4 drops of Tween-20) for 8 minutes, and then use 0.1% mercury liter solution (add 4 drip Tween-20) for 5 minutes, rinse with sterile water 5 times.
[0025] The medium for rapid propagation of clustered buds is selected as:
[0026] MS+6-BA(0.2mg / L)+IAA(0.05mg / L)+PP 333 (0.2mg / L)+sugar (30g / L)+agar (3.5g / L).
[0027] The rooting culture medium for clustering shoots is:
[0028] 1 / 2MS+IAA(0.2mg / L)+ABT(0.05mg / L)+sugar(30g / L)+agar(3.5g / L)
[0029] Culture conditions: room temperature 20°C, light intensity: 1800Lux, light time: 15h / day. Open the bottle and practice seedlings in about 20 days.
[0030] After obtaining the complete pyrethrum test-tube s...
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