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A kind of oil plant white sandalwood tissue culture rapid propagation method

A technology of oil plant and white sandalwood group, which is applied in the direction of botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of not being able to maintain the excellent traits of the female parent, prolonging the seedling cultivation cycle of white sandalwood, and the seedlings are prone to mutation, so as to ensure the transplantation The effect of planting survival rate, ensuring the quality of seedlings, and strong root system

Active Publication Date: 2019-07-26
HUNAN ACAD OF FORESTRY
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Problems solved by technology

At present, the artificial breeding of white sandalwood resources is mostly seedling propagation. However, due to the dormancy characteristics of white sandalwood seeds, the germination rate of raw seeds in the current year is extremely low, which prolongs the cultivation cycle of white sandalwood seedlings. Maintain the excellent traits of the female parent, resulting in unstable seedling quality

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] The method for tissue culture and rapid propagation of the oil-bearing plant white sandalwood comprises the following parts:

[0037] 1. Explant selection and pretreatment

[0038] Through fixed-point and regular surveys of wild white sandalwood resources for three consecutive years, an excellent individual plant with robust growth, no pests and diseases, high and stable fruit-bearing rate was selected as the mother plant.

[0039] In late November, after the fruit is ripe, it is picked and brought back to the laboratory. Remove the pulp from the seeds collected from the wild, soak them in warm water at 35°C with detergent for 30 minutes, rub the seeds clean, and rinse them under running water to ensure that the seed shells are free of grease.

[0040] Put the cleaned seeds on the ultra-clean workbench, wash them with sterile water for 2-3 times, then soak them in 75% alcohol solution for 30s, rinse them with sterile water for 2-3 times, and finally wash them with 0.1%...

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Abstract

The invention discloses a method of tissue culture and rapid propagation of oil plant white sandalwood. The method comprises the following steps: selection and pretreatment of an explant, start-up culture of a mature seed embryo, culture of an aseptic seedling with root, taking the aseptic seedling as the explant, embryogenic callus induction, somatic embryo differentiation and hyperplasia, somatic embryo maturation and germination culture.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of oil plant white sandalwood. Background technique [0002] white sandalwood ( Symplocos paniculata (Thunb.) Miq. ) is a plant of the genus Alba, also known as Osmanthus fragrans. It is a native tree species in my country. It has a strong ability to grow and has a very wide distribution range. It is distributed from Liaoning in the north to Taiwan in the south. White sandalwood has beautiful shape, graceful branches and leaves, white flowers and blue fruits, and has high ornamental value. It is an ideal tree species for landscaping; white sandalwood has strong adaptability, developed root system, can effectively prevent and control soil erosion, and is a good tree species for afforestation in shelterbelts; The roots, leaves, flowers or seeds of white sandalwood can be used as medicine, which has high medicinal value; the fruit of white sandalwood has a high oil content and con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杨艳汤玉喜唐洁李永进吴敏
Owner HUNAN ACAD OF FORESTRY
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