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Transplanting method of apple stock tissue culture seedlings

A technology for tissue culture seedlings and rootstocks, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve problems such as poor plant growth, affecting normal plant growth, and wilting of plant leaves due to dehydration and wilting , Realize annual transplanting and domestication, and ensure the effect of transplanting survival rate

Inactive Publication Date: 2018-09-28
QINGDAO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The leaves of the plants after transplanting in the existing transplanting method will lose water and wilt rapidly, the growth of the plants is not good, and the survival rate of the plants is low, which seriously affects the normal growth of the plants.

Method used

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  • Transplanting method of apple stock tissue culture seedlings
  • Transplanting method of apple stock tissue culture seedlings
  • Transplanting method of apple stock tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Move the Qingzhen No. 1 tissue culture seedlings rooted in the bottle in the culture room to another 25°C constant temperature culture room, increase the light intensity to 3-4 times of the original (about 6000-8000lx), and put the rooted bottle The seedlings were cultivated under this light intensity for 7-8 days;

[0024] (2) Gradually loosen the bottle cap, open 1 / 4 of the bottle cap every day, and open the bottle cap completely after 5 days. At this time, the humidity in the cultivation room is kept above 50%, and the bottle cap cannot be opened all at once;

[0025] (3) Take out the tissue-cultured seedlings in the bottle, wash the residual medium at the root with clean water, transplant the tissue-cultured seedlings into a cultivation box equipped with peat as a cultivation substrate, cover with a moisturizing film and a sunshade net, and cultivate them in a constant temperature culture room at 25°C , remove the shade net after 2 days, gradually remove the moi...

Embodiment 2

[0028] The cultivation substrate was replaced with peat and vermiculite in a 1:1 mixture, and the rest of the conditions were the same as in Example 1.

Embodiment 3

[0030] The cultivation substrate was replaced with vermiculite, and all the other conditions were the same as in Example 1.

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Abstract

The invention discloses a transplanting method of apple stock tissue culture seedlings. The transplanting method comprises the following steps: transferring the tissue culture seedlings rooted in bottles in a proliferation culture chamber into a constant-temperature culture chamber with a temperature of 25 DEG C, raising the illumination intensity to 3-4 times of the original illumination intensity, and culturing the rooted bottle seedlings at the illumination intensity for 7-8 days; gradually loosening bottle caps, opening 1 / 4 of each bottle cap every day, and opening each bottle cap completely after 5 days, wherein the humidity of the culture chamber is kept to be 50% or above; taking out the tissue culture seedlings in the bottles, cleaning the residual culture medium at the roots by using clear water, transplanting the tissue culture seedlings into a culture medium filled with peat, carrying out film covering for keeping moisture, carrying out covering with a sunshade net, removingthe sunshade net after culture is carried out in the constant-temperature chamber for 2 days, gradually removing the moisture keeping film after 7 days, and removing the moisture keeping film completely after two weeks; and when about 3 and 4 new leaves grow out 20 days after the transplanting, transferring the survived plants into a sunlight greenhouse or a plastic greenhouse for continuing growth. The transplanting method of the apple stock tissue culture seedlings disclosed by the invention not only ensures the transplanting survival rate of the tissue culture seedlings, but also can realize the annual transplanting and domestication of the tissue culture seedlings.

Description

technical field [0001] The invention relates to a method for transplanting apple rootstock tissue cultured seedlings. Background technique [0002] The transplanting and domestication of tissue cultured seedlings of apple rootstock is a key step in the whole tissue culture rapid propagation system. Whether and how to domesticate are directly related to the survival rate of tissue cultured seedlings transplanted. In the existing transplanting method, after transplanting, the leaves of the plants lose water and wilt quickly, the growth of the plants is not good, and the survival rate of the plants is low, which seriously affects the normal growth of the plants. Contents of the invention [0003] In order to solve the above technical problems, the invention provides a method for transplanting apple rootstock tissue cultured seedlings, so as to achieve the purpose of increasing the survival rate of transplanting and improving the growth status of the plants. [0004] To achie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G24/28A01G31/00A01H4/00A01G17/00
CPCA01G17/005A01G24/28A01G31/00A01H4/008
Inventor 马荣群黄粤沙广利葛红娟宋正旭张蕊芬
Owner QINGDAO ACAD OF AGRI SCI
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