The invention relates to a preparing method of a nickel base alloy welding wire. The components of the welding wire comprise, by weight, 0.4%-0.45% of calcium, 1.0%-1.5% of manganese, 0.15%-1.2% of silicon, equal to or less than 0.025% of phosphorus, equal to or less than 0.02% of sulfur, 28%-30% of chromium, 49%-51% of nickel, equal to or less than 0.1% of cobalt, equal to or less than 0.1% of molybdenum, equal to or less than 0.1% of titanium, equal to or less than 0.1% of aluminum, 4.0%-5.0% of wolfram, and the rest of iron. The preparing method is alloy smelting, electro-slag re-smelting, hot forging cogging, hot rolling annealing, acid pickling, coping, cold draw and hydrogen back, and the electro-slag re-smelting adopts a calcium fluoride, aluminum oxide and calcium oxide slag system. The preparing method of the nickel base alloy welding wire can produce the fine welding wire which can satisfy the requirement of the performance, and the yield is high.