The invention relates to a method for detecting
poloxamer residual quantity in a recombinant human
urokinase raw material for injection. The method comprises the following steps: (1) a sample solutionpreparation method: adding overnight frozen
acetonitrile into a
raw material sample, oscillating and ultrasonically treating for 2-4 minutes, oscillating for 20-40 seconds, centrifuging at 8000-9800rpm for 1-3 minutes, and taking a supernatant, wherein the ratio of the volume of the original sample to the volume of the
acetonitrile is within 1-3; 2) preparing a standard substance solution: taking, weighing and preparing a
poloxamer standard substance into standard substance solutions, wherein the concentrations of the standard substance solutions are 2 mg / ml, 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, 0.1 mg / ml, 0.04 mg / ml, 0.02 mg / ml, 0.01 mg / ml and 0.005 mg / ml in sequence; and 3) detection: respectively taking a
standard solution and a sample solution, performing injecting into a high performanceliquid chromatograph, recording a chromatogram detected by CAD, drawing a
standard curve by taking the concentration of the standard substance solution as an abscissa and the
peak area as an ordinate,calculating the content of
poloxamer in the sample solution by using an external standard method, and converting to obtain the content of poloxamer in the recombinant human
urokinase raw material forinjection.