The invention discloses a rotation type vacuum sealing system comprising a support frame, an air control device, sealing devices, a rotating device, and an annular sealing-connecting device; the support frame comprises a support plate and support rods; the air control device comprises a main pipeline, a vacuum-pimping mechanism and an inflating mechanism; each sealing device comprises a sealing mechanism, a rotating mechanism, a driving mechanism, and a control valve; the rotating device comprises a hollow shaft, a shaft sleeve, driving wheels, and magnetic fluid sealing elements, the lower end of the hollow shaft is connected with the control mechanism, the upper end of the hollow shaft is connected with the shaft sleeve, and the upper end of the shaft sleeve communicates with the main pipeline through the control valve; and the annular sealing-connecting device comprises an annular heater, an oxyhydrogen machine, a gas pipeline, and a heating holder. According to the rotation type vacuum sealing system, multiple sealing devices are arranged on the support plate, and the synchronous sealing of multiple test tubes is realized through the rotating mechanism to facilitate to enhancesealing efficiency is enhanced; and the test tubes can be rotated in a vacuum through the rotating mechanism, an annular heater and the test tubes are cooperatively used for heating to avoid manual control, and the balance and security of heating sealing is enhanced to facilitate synchronous sealing of the multiple test tubes.