The invention discloses a magnetic isolation sheet applied to wireless charging and near field communication (NFC). The magnetic isolation sheet comprises at least one magnetic isolation unit, whereinthe magnetic isolation unit comprises a soft magnetic strip and a double-side adhesive tape, wherein the soft magnetic strip is of a sheet structure with two exposed surfaces, the double-side adhesive tape covers one of the exposed surfaces, and the other exposed surface of the soft magnetic strip is pressed to form a mesh-shaped line. The invention also discloses a fabrication process of the magnetic isolation sheet applied to wireless charging and NFC. The fabrication process comprises the steps of constructing a magnetic isolation unit, in which the double-side adhesive tape is pasted ontoone of the exposed surfaces of the soft magnetic strip by taking the soft magnetic strip of the sheet structure with the two exposed surfaces as an element, and the other exposed surface of the softmagnetic strip is pressed to form the mesh-shaped line; and constructing the magnetic isolation sheet. The magnetic isolation sheet applied to wireless charging and NFC, fabricated by the fabricationprocess has the advantages of thin thickness, good magnetic isolation capability, high charging efficiency, NFC working frequency stability and high product stability, and the process and the materialcost are greatly reduced.